FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686768

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686768
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1096877
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50690.46213021150046907No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC27420.24998245017444984No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG25020.22810214818981528No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCC21710.19792556503600675No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21190.19318483293933594No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT20830.18990278764164079No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG19810.18060365929817107No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG18400.16774898188219828No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC18310.16692847055777446No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA18270.16656379885803058No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT18250.16638146300815862No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG17800.16227890638603965No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG16770.15288861011763397No Hit
ATCTGGGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC16720.1524327704929541No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG16410.14960656481993878No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTCCCTCCGCGTCCGGGTCTCCTGGACAGTCAGTCAC16300.14860371764564304No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT15770.14377181762403624No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA15760.14368064969910027No Hit
GTCTCTGGTGGCTCCATGAGTATTAATAGTTTCTGTTGGGGCTGGATCCG15560.1418572912003807No Hit
GATTATTACTGCAACTCATATGCAGGCAGCAACAATCTGGGGGTGTTCGG15330.13976042892685323No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC15250.13903108552736543No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15120.13784590250319773No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15050.13720772702864587No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT14950.13629604777928608No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG14520.13237582700703907No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCCCAGATTGTTG14390.13119064398287136No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACTGGAAGCAGCAGTGACGTT13780.12562940056177677No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC13770.12553823263684077No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC13510.12316786658850537No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG13470.12280319488876146No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA13440.12252969111395352No Hit
GTATTAATAGTTTCTGTTGGGGCTGGATCCGCCAGCCCCCAGGGAAGGGG13310.12134450808978581No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACAC13100.11942998166613031No Hit
CCCCCAGATTGTTGCTGCCTGCATATGAGTTGCAGTAATAATCAGCCTCA12740.11614793636843511No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG12710.11587443259362719No Hit
TCTCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCAACTCATAT12580.11468924956945947No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA12300.11213654767125211No Hit
CTCCTGCACTGGAAGCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGCACCCAGGCAAAGC12230.11149837219670027No Hit
ATAATACACAGCTGTGTCTCCGGCACTCACATAGCTCAGTCTCAAGGAGAACTGGTTCTTGGACGTGTCTACGGA12220.11140720427176429No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC12120.11049552502240452No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12050.10985734954785267No Hit
GTCAGTCACCATCTCCTGCACTGGAAGCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACAGCA11970.10912800614836485No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC11790.10748698349951726No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC11680.10648413632522152No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC11510.10493428160130991No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC11490.10475194575143795No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11010.10037588535451104No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAT353.9463193E-580.5745170
GTGGTAT3300.049.533071
TGGTATC3550.046.0301172
GGTACGT357.785216E-444.08078868
GGTATCA13100.043.932241
GTATCAA33650.038.861011
GTACGTA450.001466108438.75832769
TATCAAC38250.034.2656062
ATCAACG40300.032.165593
TCAACGC40700.031.7571814
AACGCAG42150.030.8204776
CAACGCA42200.030.785365
GGATGGG10100.029.31795170
ACGCAGA44550.029.2364467
ACGGGAT4200.029.15984316
CGCAGAG44800.029.149218
AGAGTAC45400.028.83625411
CAGAGTA46050.028.43052310
GCAGAGT46000.028.3148659
TAGCCCA659.4125036E-426.1607385
Produced by FastQC (version 0.11.5)