FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686769

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686769
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1734496
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAAAGGGCCGTGTCCTCGGCTCTCAGGCTGTTCACTTGCAGATAAA48050.27702571813368265No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC40610.23413141339040272No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT39350.22686705532904083No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC34460.1986744276147077No Hit
CTCCTGTACAGCCTCTGGATACACCTTTACCAACTTTGCCATGGCCTGGGTCCGCCGGGCTCCAGGGAAGGGG33800.19486928767780382No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT29070.1675991181299928No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28860.16638839178643247No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG28580.16477408999501872No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC27870.1606806818810767No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA27630.1592969946312935No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT25560.1473626921019132No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA25180.14517185395642307No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25150.14499889305020017No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24440.14090548493625815No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA24390.14061721675921998No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG24120.13906056860321384No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23650.13635084773905504No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG23030.1327763223437817No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT22720.13098905964614505No Hit
GTTTATTACTGTCAGCATTATGGTAGTTCACGGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA22380.1290288360422855No Hit
GTCTGGGACAGACTTCACTCTCACCATCAAGAAGCTGGACCCTGAAGATT22110.12747218788627937No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG22020.12695330516761066No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT21940.12649207608434956No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21660.12487777429293581No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21650.12482012065752818No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT21360.12314816523070679No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT21000.12107263435603197No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20690.1192853716583953No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGACGCCAGTGGCCCTGCT20480.11807464531483497No Hit
CTTTATTACTGTGCGAAAGGATGGGGCTTTGGTGACTACTACTTTGACTC20230.11663330442964412No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC20220.11657565079423649No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19470.1122516281386639No Hit
CTATGTAGACTCCGTGAAGGGCCGCTTCAACATCTCCAGAGACAATTCCAAGAACACCCTTTATCTGCAAGTGAA19340.11150213087836468No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19170.11052201907643489No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG19000.1095419072745051No Hit
TCCTTGGGGCTGAAGCCGCGTGCCAGGCACGTCAGCGTCACCAGCTCGTT18340.10573676733760123No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT18300.10550615279597071No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC18020.10389185100455693No Hit
GACTTCACTCTCACCATCAAGAAGCTGGACCCTGAAGATTCTGCAGTTTATTACTGTCAGCATTATGGTAGTTCA17920.1033153146504806No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG17900.10320000737966534No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG17860.1029693928380348No Hit
GTCTACATAGTAGGTGGTAGTAGCATGAGGACTAATTGTGGAGACCCACT17850.10291173920262717No Hit
CTATTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATTCAGCAGGGCCA17750.10233520284855081No Hit
CATTGACATGGGTGGGTTTACCCGCCAAGCGGTCGATGGTCTTCTGTGTGAAGGCCAGCGGCAGGGCCTCGTGGC17680.10193162740069737No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG17590.10141274468202868No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18850.041.208311
TCGTATG4200.037.74922640
GTATCAA45050.037.033991
TATCTCG4250.037.0185836
TCTATAC558.560215E-636.843163
TCTGGAG20000.036.8006270
CGTATGC4050.035.80645841
ATCTCGT4450.035.40756237
GACGGAC5150.033.48749526
ACGGGTT1850.032.8525416
CGAGACG5200.032.48237223
TATACAC5550.031.6430743
CCTATCT4850.031.63399334
ATGCCGT4650.031.35451344
CTATCTC4900.031.34861635
CGGACTC5300.031.3052528
TATCAAC53550.031.2817132
CCGTCTT4500.031.02049447
ATCAACG54850.030.8481713
ATCTCCG5700.030.79881710
Produced by FastQC (version 0.11.5)