FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686770

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686770
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1734496
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG54470.3140393520653838No Hit
GTAATAAAGGGCCGTGTCCTCGGCTCTCAGGCTGTTCACTTGCAGATAAA45430.26192046565688243No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37030.21349141191446966No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC34000.1960223603859565No Hit
CTCCTGTACAGCCTCTGGATACACCTTTACCAACTTTGCCATGGCCTGGGTCCGCCGGGCTCCAGGGAAGGGG33950.19573409220891833No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32430.18697073962695793No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC30670.17682369979521428No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA26110.15053364204933306No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT25310.1459213512167223No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT25250.14557542940427654No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23990.1383110713429146No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA23230.13392939505193438No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22130.12758749515709464No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT22090.1273568806154641No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG21970.12666503699057247No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC21960.12660738335516483No Hit
GTCTGGGACAGACTTCACTCTCACCATCAAGAAGCTGGACCCTGAAGATT21880.12614615427190376No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC21370.12320581886611442No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21280.12268693614744572No Hit
GTTTATTACTGTCAGCATTATGGTAGTTCACGGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAACGAA21060.12141855616847777No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG20820.12003486891869453No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT20400.11761341623157388No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG20370.11744045532535101No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG20320.11715218714831283No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT19480.11230928177407154No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGACGCCAGTGGCCCTGCT19350.11155978451377228No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT18810.10844648820176005No Hit
GTACCTGACTTGGGCATCCCGGCAGGAGCCCAGCCAGGGCACCACCACCT18700.10781229821227606No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT18600.10723576185819973No Hit
ATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGTCCACCT18540.10688984004575393No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA18540.10688984004575393No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG18130.10452604099404092No Hit
GTAATAAACTGCAGAATCTTCAGGGTCCAGCTTCTTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA17560.10123978377580578No Hit
CTTTATTACTGTGCGAAAGGATGGGGCTTTGGTGACTACTACTTTGACTC17470.10072090105713706No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGG657.528326E-444.3812870
GGTATCA17950.040.051581
GTATCAA43850.037.7164651
TCTGGAG21000.035.71636270
TATCAAC52550.031.2054332
ATCAACG53450.030.354723
TCAACGC54150.030.013364
CAACGCA54800.029.9026175
AACGCAG55250.029.6590656
CGGTCAA500.00580190229.27481363
CGCAGAG57350.028.4554488
ACGCAGA57600.028.390487
AGAGTAC58400.027.59503711
GCAGAGT60000.027.1424739
GACAGGA3100.026.130881
CAGAGTA65500.024.75966510
CGGCTCT19500.024.75675621
CCGTGTC18800.024.75282912
GCAGATA19700.024.74706842
ATTGTCT20100.024.6843364
Produced by FastQC (version 0.11.5)