FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686771

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686771
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1872633
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAATCAGCCTCATCCTCAGGCTGGAGCCCACTGATGGCCAGGGAGG79750.4258709528241786No Hit
GCCTTGGGCTGACCGAGGACGGTCAGCTGGGTGCCTCCTCCGAACACAGCACCTTTCAGGCTGTCATCCCATG79750.4258709528241786No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC70310.3754606481889404No Hit
GATTATTACTGTGCAGCATGGGATGACAGCCTGAAAGGTGCTGTGTTCGG69190.3694797645881494No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTATTCATTAGCATATACA63430.33872093464122444No Hit
GTATATGCTAATGAATAGCCTGAGAGCCGAGGACACGGCCGTATATTACT60920.32531734728588035No Hit
AGCATATACAGCGTGTTCTTGGGATTGTCCCTGGAGATGGTGAACCGGCCCTTCACGGAGTCTGCGTAGTATGTG53230.2842521732768781No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC47500.25365354556925995No Hit
GTATATTACTGTGCGAAAGATTTTTTCTTCTTTCCAGTGGGGGGTACTAT44870.2396091492566883No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT42720.22812798877302706No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCGTAAGT40160.21445739768550487No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT39690.21194756260303005No Hit
CTTCATGCGTGACCCGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG39650.21173395961728753No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAGCTGGGTGCCTC38660.20644728572015977No Hit
CTATAGGGACGTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGTCTTCCCG38250.20425785511629882No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT35660.1904270617894697No Hit
TCCCTATAGTACCCCCCACTGGAAAGAAGAAAAAATCTTTCGCACAGTAA35040.1871162155104604No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCCGGCAGCTGTAGCTTCT33900.18102853041679817No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA33790.1804411222060062No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG33470.17873229832006593No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG33040.17643606622333366No Hit
CTCCTGTGCAGCCTCTGGATTCAGCTTTAGCAGCTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG32960.1760088602518486No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT32950.17595545950541297No Hit
GTGCTCCCTTCATGCGTGACCCGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG32340.1726980139728393No Hit
CTCCAGGGACAATCCCAAGAACACGCTGTATATGCTAATGAATAGCCTGAGAGCCGAGGACACGGCCGTATATTA30120.16084304826412865No Hit
CCCCTGAGGGCCGCTGATTATTACTATAGATGAGGAGTCTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTTTA30000.16020223930690103No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG29460.1573185989993768No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC29320.15657098854927792No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG28670.15309994003096175No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTGAC28240.1508037079342295No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTACGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG28120.1501628989770019No Hit
GTTTGGTGGTCTCCACTCCCACCTTGACGGGGCTGCCATCTGCCTTCCAG27820.14856087658393288No Hit
GCCCTGGGATTATCAGGTGTTTTCATTTGGTGATCAGGACTGAACAGAGA26790.1430605997010626No Hit
CCCTTCATGCGTGACCCGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG26440.14119157357581544No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC26050.13910894446482575No Hit
GGCTCAGGTAGCTGCTGGCCGCATACTTGTTGTTGCTTTGTTTGGAGGGTTTGGTGGTCTCCACTCCCACCTTGA25660.13702631535383603No Hit
GGTGTGTCTCGTAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA25500.13617190341086588No Hit
GCATGGGATGACAGCCTGAAAGGTGCTGTGTTCGGAGGAGGCACCCAGCTGACCGTCCTCGGTCAGCCCAAGG25060.133822270567698No Hit
GTGTTACTTCCGATGTTGGACCTGCTTCCAGAACAAGAGATGGTCACCCT24900.13296785862472785No Hit
GGGCTGGATGACTTGGGATGGGGGCAGAGACCCCTCCCCTGGGAACCTGC24460.13061822578155996No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA24210.12928320712066915No Hit
ACACTGGTGTGTCTCGTAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC24160.12901620338849096No Hit
GTCTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTTTACAGTGTTACTT24150.12896280264205534No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGC23950.12789478771334264No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT23690.1265063683060162No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC23490.1254383533773035No Hit
GTACTATAGGGACGTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGTCTTCC23390.12490434591294716No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG23160.1236761287449276No Hit
CTGTAAACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAGACTCCTCATC23160.1236761287449276No Hit
CTATAGTACCCCCCACTGGAAAGAAGAAAAAATCTTTCGCACAGTAATATACGGCCGTGTCCTCGGCTCTCAGG22910.12234111008403675No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT22220.11865645857997804No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG21860.11673403170829522No Hit
GATTATTACTATAGATGAGGAGTCTGGGGGCCGTTCCTGGGAGCTGCTGG21770.1162534249903745No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGC21720.11598642125819636No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC21520.11491840632948368No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC21460.11459800185086987No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT21240.11342318542928594No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC21180.11310278095067214No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT21000.11214156751483072No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCA20880.11150075855760311No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC20780.11096675109324677No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCGTAAGTGACTTCTACC20720.11064634661463299No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGG20470.10931132795374214No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC20250.10813651153215818No Hit
ATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCAGGCTGGAGCCCAC19930.10642768764621792No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCAGCTTTAGCAGCTATGCCATGAGCTGGGTCCG19920.10637428689978229No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC19830.10589368018186157No Hit
CCCCTCCCCTGGGAACCTGCAGCTCCAGGCCCCTGTGGGTGGGGTGAGGG19760.10551987495681214No Hit
GGATATTTATTGAGAAGGGCTGGATGACTTGGGATGGGGGCAGAGACCCCTCCCCTGGGAACCTGCAGCTCCAGG19700.10519947047819836No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCCGGCAGCTGTAGCTTCTGT19560.10445186002809947No Hit
GACCGAGGACGGTCAGCTGGGTGCCTCCTCCGAACACAGCACCTTTCAGG19450.1038644518173075No Hit
GTGTTCGGAGGAGGCACCCAGCTGACCGTCCTCGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACC19450.1038644518173075No Hit
GTGTGAGGTGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCT19370.10343724584582242No Hit
GTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG19120.1021022271849316No Hit
GGCCATCAGTGGGCTCCAGCCTGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGCCTGAAAGGTG19040.10167502121344653No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGGCCCCACGTCCCTATAGTACCCCCCA18990.10140801748126835No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC18980.10135461673483272No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA18880.10082060927047638No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA18850.10066040703116948No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC18800.10039340329899131No Hit
GAAGTAACACTGTAAACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAGA18730.10001959807394188No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTAAT1650.049.93143532
GGTATCA14550.047.7736131
ACCGTTT606.0283754E-446.42935670
TCGTATG1750.045.32901440
GTGGTAT3600.044.7044141
TGGTATC3700.043.497352
CTTTCCA25950.040.79342370
CGTATGC1900.039.96635441
TCGCTAA2100.039.20874431
CACTCGC2200.038.94740328
GCCGTCT2050.038.91113746
GCTAATC1950.038.7444333
ACTCGCT2250.038.09107229
ATGCCGT2000.038.05922744
GCTTGTA901.3278623E-1038.046311
ATCTCGT1900.038.03501537
GTATCAA35600.037.3195841
AATCTCG1950.037.03589636
ACACTCG2400.035.6922527
CCGTCTT2250.035.49461447
Produced by FastQC (version 0.11.5)