FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686772

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686772
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1872633
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC69630.37182939743131727No Hit
GATTATTACTGTGCAGCATGGGATGACAGCCTGAAAGGTGCTGTGTTCGG65160.347959263774589No Hit
GCCTTGGGCTGACCGAGGACGGTCAGCTGGGTGCCTCCTCCGAACACAGCACCTTTCAGGCTGTCATCCCATG63210.3375461182196405No Hit
GTAATATACGGCCGTGTCCTCGGCTCTCAGGCTATTCATTAGCATATACA58820.3141031905343973No Hit
GTATATGCTAATGAATAGCCTGAGAGCCGAGGACACGGCCGTATATTACT57960.3095107263409328No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC54760.29242248748153No Hit
AGCATATACAGCGTGTTCTTGGGATTGTCCCTGGAGATGGTGAACCGGCCCTTCACGGAGTCTGCGTAGTATGTG46270.24708525375767704No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT43950.23469628058461No Hit
GTATATTACTGTGCGAAAGATTTTTTCTTCTTTCCAGTGGGGGGTACTAT42450.226686168619265No Hit
GGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTT40940.21862265590748428No Hit
CTTCATGCGTGACCCGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG36680.19587393792590432No Hit
CTATAGGGACGTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGTCTTCCCG36170.193150499857687No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTGAC35940.19192228268966743No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCGTAAGT35660.1904270617894697No Hit
CTCCTGTGCAGCCTCTGGATTCAGCTTTAGCAGCTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGG33980.18145573638828325No Hit
GTAATAATCAGCCTCATCCTCAGGCTGGAGCCCACTGATGGCCAGGGAGG33420.17846529458788774No Hit
GTGCTCCCTTCATGCGTGACCCGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG32810.17520784905531409No Hit
TCCCTATAGTACCCCCCACTGGAAAGAAGAAAAAATCTTTCGCACAGTAA32100.1714163960583841No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT31760.16960077067957255No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGGTCAGCTGGGTGCCTC31570.16858615649729553No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCCGGCAGCTGTAGCTTCT31450.1679453475400679No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC29630.1582264116887826No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG29460.1573185989993768No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG29130.1555563743670009No Hit
CTCCAGGGACAATCCCAAGAACACGCTGTATATGCTAATGAATAGCCTGAGAGCCGAGGACACGGCCGTATATTA28200.15059010494848699No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT28020.14962889151264558No Hit
CCCCTGAGGGCCGCTGATTATTACTATAGATGAGGAGTCTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTTTA27760.14824047210531907No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC27240.14546363329066614No Hit
GTTTGGTGGTCTCCACTCCCACCTTGACGGGGCTGCCATCTGCCTTCCAG27170.1450898280656167No Hit
GCCCTGGGATTATCAGGTGTTTTCATTTGGTGATCAGGACTGAACAGAGA27140.1449296258263098No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG26750.14284699671532008No Hit
GTCTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTTTACAGTGTTACTT26140.13958955118274644No Hit
GTGTTACTTCCGATGTTGGACCTGCTTCCAGAACAAGAGATGGTCACCCT26010.1388953414790832No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA25890.1382545325218556No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25790.13772052505749924No Hit
GGCTCAGGTAGCTGCTGGCCGCATACTTGTTGTTGCTTTGTTTGGAGGGTTTGGTGGTCTCCACTCCCACCTTGA25640.13691951386096476No Hit
CTGTAAACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAGACTCCTCATC25400.13563789594650955No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGC25130.13419607579274745No Hit
GGTGTGTCTCGTAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA24900.13296785862472785No Hit
GGGCTGGATGACTTGGGATGGGGGCAGAGACCCCTCCCCTGGGAACCTGC24630.13152603847096575No Hit
CCCTTCATGCGTGACCCGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG24340.12997741682433236No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA24200.1292298063742335No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTACGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG23510.12554515487017479No Hit
GTACTATAGGGACGTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAGCATCCCCGACCAGCCCCAAGGTCTTCC23150.12362272799849197No Hit
GCATGGGATGACAGCCTGAAAGGTGCTGTGTTCGGAGGAGGCACCCAGCTGACCGTCCTCGGTCAGCCCAAGG22890.12223430859116548No Hit
GTGTGAGGTGCTGTTGGAGTCTGGGGGAGGCTTGGTACAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCT22780.12164690038037351No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGC22680.12111289291601719No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT22550.12041868321235394No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGC22230.11870985932641367No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCGAGGACGG21870.11678743245473085No Hit
GTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG21570.11518541006166184No Hit
GAAGTAACACTGTAAACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAGA21350.1140105936400779No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG21350.1140105936400779No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG21290.11369018916146409No Hit
GGGTGAGGGTCGGGGGCCTAAGAGCATTCTGCAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC21130.11283577721849397No Hit
GAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGCTTTTTCTTGT21100.11267557497918707No Hit
GATTATTACTATAGATGAGGAGTCTGGGGGCCGTTCCTGGGAGCTGCTGG21070.11251537273988016No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC21000.11214156751483072No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA20690.11048614437532608No Hit
CTATAGTACCCCCCACTGGAAAGAAGAAAAAATCTTTCGCACAGTAATATACGGCCGTGTCCTCGGCTCTCAGG20540.10968513317879157No Hit
ACACTGGTGTGTCTCGTAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC20210.10792290854641567No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA20120.10744230182849496No Hit
GTGTTCGGAGGAGGCACCCAGCTGACCGTCCTCGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACC20080.10722869884275243No Hit
GATCAGGACTGAACAGAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGC19820.10584027943542594No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC19720.10530627197106962No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC19610.10471886376027764No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT19610.10471886376027764No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC19490.10407805480305003No Hit
GGCCATCAGTGGGCTCCAGCCTGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGCCTGAAAGGTG19390.1035440473386937No Hit
GCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGATTCAGCTTTAGCAGCTATGCCATGAGCTGGGTCCG19380.10349064659225807No Hit
GAGTTTGGGCTGAGCTGGCTTTTTCTTGTGGCTATTTTAAAAGGTGTCCA19370.10343724584582242No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC19260.10284983763503046No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCATACTT19090.1019420249456247No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT19080.10188862419918907No Hit
GGCAGAGGGTGACCATCTCTTGTTCTGGAAGCAGGTCCAACATCGGAAGTAACACTGTAAACTGGTACCAGCAG19010.10151481897413962No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCAGCCTGAGGATG18930.10108761300265455No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3700.053.6531871
TGGTATC3850.050.6643032
GGTATCA14600.045.4797551
GCTTGTA1550.039.747521
GTATCAA37200.039.2874831
CGTGTTA1100.038.07354452
TATCAAC42000.034.7909742
CTTTCCA25000.034.61630270
ATCAACG44250.032.9243623
TCAACGC45050.032.481974
CAACGCA45050.032.404355
AACGCAG45700.032.0182656
GACAGGT2950.031.3264371
CGCAGAG48450.030.2009268
CAGAGTA49200.029.94500710
AGAGTAC49150.029.90672111
GCAGAGT49950.029.2916459
CCCCGAT1255.58002E-428.60851570
TATTCAT20250.028.47799333
CATTAGC20250.028.20173537
Produced by FastQC (version 0.11.5)