FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686773

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686773
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1546083
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT69580.4500405217572407No Hit
GTATAATAAGTGGCCTCAGATCACCTTCGGCCAAGGGACACGAGTGGAGATTGAACGAACTGTGGCTGCACCAT68580.44357256369806797No Hit
CTTATTATACCGCTGACAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAAAT60920.3940280049648046No Hit
GTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA60710.39266973377237835No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC58080.37565900407675396No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG46660.30179492304100103No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC44340.28678926034372026No Hit
CATCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGATTTCA42370.27404738296714987No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC39890.25800684698040144No Hit
CTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGATTTCACT38170.2468819591186243No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG38030.2459764449903401No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG37350.24157823351010263No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGGC37090.23989656441471774No Hit
GTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTTAGCCTGGTA36710.23743874035223206No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT35820.2316822576795683No Hit
GAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTC34990.2263138524904549No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA34600.22379134884737756No Hit
GTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAG34230.2213982043654836No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCT33460.21641787665992057No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG33330.2155770421122281No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC33280.21525364420926948No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT32980.21331325679151766No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG32140.20788017202181255No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT30870.19966586528666316No Hit
GGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGG30420.1967552841600354No Hit
GTTTATTACTGTCAGCGGTATAATAAGTGGCCTCAGATCACCTTCGGCCA30180.19520297422583394No Hit
GTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCACTGCCA29760.19248643184098138No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTCAATCTCCACTCGTGTCCCTTGGCCGAAGGTGATCTGAGGCCA27830.18000327278677794No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT27490.1778041670466592No Hit
GGATACCAGTGGCCCTGGTGGATGCACCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGCTGGTACCAGG27450.1775454487242923No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATT27080.17515230424239836No Hit
GTGCAGCCACAGTTCGTTCAATCTCCACTCGTGTCCCTTGGCCGAAGGTGATCTGAGGCCACTTATTATACCG26580.17191832521281197No Hit
GCTCAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCT26320.17023665611742708No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCGGTATAATAAGTGG26030.168360948280267No Hit
GTCAGAGTGTTAGCAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTG25300.16363933889707086No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA24680.15962920490038374No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT22900.1481162395550562No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22830.14766348249091413No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG22790.14740476416854723No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT22770.14727540500736377No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG22630.1463698908790796No Hit
GTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCAGTGGCCCTGGT22170.14339463017186013No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC22110.14300655268830975No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT21570.13951385533635646No Hit
GAGTGAAATCTGTCCCAGACCCACTGCCACTGAACCTGGCTGGGATACCA21520.1391904574333978No Hit
CAGTAATAAACTGCAAAATCTTCAGACTGCAGGCTGCTGATGGTGAGAGTGAAATCTGTCCCAGACCCACTGCCA20560.13298121769659196No Hit
GCCACAGTTCGTTCAATCTCCACTCGTGTCCCTTGGCCGAAGGTGATCTG20090.12994127740878078No Hit
GTGTTAGCAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCA19910.1287770449581297No Hit
GAGTGTTAGCAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATC19840.12832428789398756No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19810.1281302491522124No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC19770.12787153082984548No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA19500.12612518215386884No Hit
GGACACGAGTGGAGATTGAACGAACTGTGGCTGCACCATCTGTCTTCATC19450.12580178425091018No Hit
GCAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTT19230.1243788334778922No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT19130.12373203767197492No Hit
TCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGATTTCACT19090.12347331934960801No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA18740.12120953402889754No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC18510.11972190367528782No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18410.11907510786937053No Hit
GGTTTCTGCTGGTACCAGGCTAAGTTGCTGCTAACACTCTGACTGGCCCTGCAGGAGAGGGTGGCTCTTTCCC18310.11842831206345325No Hit
CTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTGATGACGCAGTC18200.11771683667694424No Hit
CCTTCGGCCAAGGGACACGAGTGGAGATTGAACGAACTGTGGCTGCACCA18160.11745811835457735No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT18140.11732875919339388No Hit
GCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTGATGACGCAGTCTCCAGCCACC17950.11609984716215106No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA17890.1157117696786007No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT17710.11454753722794961No Hit
ATCTCCACTCGTGTCCCTTGGCCGAAGGTGATCTGAGGCCACTTATTATA17710.11454753722794961No Hit
CAGTTAGGACCCAGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC17690.11441817806676613No Hit
CCCTGTCTGTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTTAGC17640.11409478016380752No Hit
CCAGTGGCCCTGGTGGATGCACCATAGATGAGGAGCCTGGGAGCCTGGCC17600.1138360618414406No Hit
GTTCAGTGGCAGTGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGCC17470.11299522729374814No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT17460.1129305477131564No Hit
CACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGATTTCACTCTCACCATCAGCAG17450.11286586813256468No Hit
GAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTAGCAGCAACTTAGCCTGGTACCAGCAGAAACCTGGCCAGG17430.11273650897138122No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG17410.11260714981019776No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA17350.11221907232664739No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC17220.11137823777895495No Hit
CCCAGATACCACTGGAGAAATAGTGATGACGCAGTCTCCAGCCACCCTGTCTGTGTCTCCAGGGGAAAGAGCCAC17060.1103433644894873No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACTGGAGAAATAGTGATGACGCAGTCTC17030.11014932574771212No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC16990.1098906074253452No Hit
CCTCATCTATGGTGCATCCACCAGGGCCACTGGTATCCCAGCCAGGTTCAGTGGCAGTGGGTCTGGGACAGATTT16740.10827361791055203No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA16590.1073034242016761No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT16510.1067859875569423No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC16400.10607451217043329No Hit
CTCCCAGGCTCCTCATCTATGGTGCATCCACCAGGGCCACTGGTATCCCA16060.10387540643031455No Hit
AGACGGAACCATGGAAGCCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCT15900.1028405331408469No Hit
CACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCGGTATAATAAGTGGCCTCAGATCAC15770.10199969859315446No Hit
CTCGTGTCCCTTGGCCGAAGGTGATCTGAGGCCACTTATTATACCGCTGA15720.10167630069019581No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT15660.10128822320664545No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTATC350.001327344439.55235744
GTATCAA35550.039.0284351
CGTATCA400.002541378834.65155845
GGTATCA17800.034.2814671
ATCAACG41850.033.4779363
TATCAAC42250.033.241672
TCAACGC42200.033.1194954
CAACGCA42800.032.65525
AACGCAG42950.032.6205256
TCTTATA2350.031.9129622
ACGCAGA44550.031.3663647
CGCAGAG45150.030.8720518
GCTTGTA1550.030.790921
CGTATGC2700.030.65421741
AGAGTAC46350.030.22080611
TATCTCG2400.030.00927436
GCCGTCT2800.029.7443746
TCGTATG2550.029.71372840
GCAGAGT47050.029.6977929
ATCTCGT2350.029.20831337
Produced by FastQC (version 0.11.5)