FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686776

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686776
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3557168
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG174920.4917394961385012No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC153310.43098892152408885No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCAGTGGAACCAGCAGTGACGTT141380.3974510059687931No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG135590.3811740125852926No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC133390.37498931734458424No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACGAGAGCGTTGCTGCTTGTATATGAG130090.3657122744835217No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG128130.3602022732690725No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC122110.3432786981104069No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACGAGAGCGTTG119830.33686910486094557No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT113100.3179495598745969No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG93650.2632712314965163No Hit
TCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATAT93530.2629338844833868No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG85150.23937581806650685No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACGAGAGCGTTGC77660.21831974199700435No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC75760.2129784142891199No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT75130.21120734247018977No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT70770.19895040099314962No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA69290.19478978783121853No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC68870.19360907328526514No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC66690.18748060254674506No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT66270.18629988800079164No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG65240.18340432613809637No Hit
GTCGATCACCATCTCCTGCAGTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACGGCA64900.18244850960089598No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG64890.18242039734980187No Hit
CTCCTGCAGTGGAACCAGCAGTGACGTTGGTGGTTATAACTATGTCTCCTGGTACCAACGGCACCCAGGCAAAGC64290.18073366228415413No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG64200.18048065202430696No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG63160.17755697791051758No Hit
GATTATTACTGCAGCTCATATACAAGCAGCAACGCTCTCGTGGTGTTCGG61670.17336825249749238No Hit
GCCCAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTAGAGCCAGAGTAG61210.17207508894716247No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC60210.16926386383774958No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC59970.1685891698114905No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG59650.16768957777647836No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA58250.16375386262330033No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA56160.15787840214462742No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT54250.1525089621856488No Hit
GGTTATAACTATGTCTCCTGGTACCAACGGCACCCAGGCAAAGCCCCCAAACTCATGATTTATGAGGTCAGTAAT53570.15059732911124807No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT52580.1478142162529293No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT52450.14744875698870563No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT50880.1430351335669274No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC49570.1393524286735965No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATGGTCAGGGAGG49020.13780625486341944No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT48980.13769380585904292No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC48660.1367942138240308No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC47700.13409543771899443No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT46580.13094686559645202No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA45270.12726416070312113No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA45200.12706737494546225No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC44210.12428426208714348No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT43570.12248507801711923No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC43180.1213887002244482No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC41860.11767788308002321No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC41330.11618793377203439No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC41020.11531645398811638No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG40690.11438874970201014No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC40340.11340482091371563No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG40270.11320803515605672No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC39560.11121206532837358No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAAC39460.1109309428174323No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC38400.10795104420145464No Hit
TCGCTACTCTGGCTCTAAGTCTGGCAACACGGCCTCCCTGACCATCTCTG37830.1063486458890893No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCACGAGAGCGTTGCTGCTTGTATATGAGCTGCAGTAAT37800.10626430913580692No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA37610.10573017636501846No Hit
GTATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGA37030.104099665801559No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA36660.10305951251107623No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC36550.1027502777490408No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT35880.10086675692573417No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT9350.040.173341
GGTATCA33300.036.403321
AATGGTA19200.035.74100570
GTATCAA71500.035.4845771
TATCAAC79450.031.7955472
ATCAACG79800.031.5144583
TCAACGC81050.030.8522074
CAACGCA81700.030.6045995
AACGCAG83100.030.2938166
ACGCAGA87950.028.6232647
TTGCGAC500.00679311628.34555659
CGCAGAG88950.028.3398238
AGAGTAC90550.028.06193511
AAAGTCA17800.027.821170
GCAGAGT91600.027.5191759
AGTAATA83000.027.18997470
GTGCTAG7150.026.2671811
GTCTAAC801.02593614E-425.6105041
TGGTATC14700.024.8482022
AGTGCGT700.001425443224.3652368
Produced by FastQC (version 0.11.5)