FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686779

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686779
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1388359
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTATTACTGTGCATCATGGGATGACAGCCTGAATGGTTCTGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTC94360.6796513005641913No Hit
GTAATAAAGAGACTCATCCTCAGACTGGAGTCCACTGATGGCCAGGGAGG86420.6224614815044236No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC63290.4558619204398862No Hit
GCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCAC54560.3929819304661114No Hit
GTTCTGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTGGGTCAGCCC48900.3522143768290478No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT45490.32765300617491583No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC44660.32167472534121216No Hit
CCACAGAACCATTCAGGCTGTCATCCCATGATGCACAGTAATAAAGAGAC43730.31497616970826714No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG43050.31007830107342554No Hit
GTAATAGACAGCCGTGTCCTCGGCTCTCAGGCTGCTCATTTGCAGATACA39870.28717356245754877No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTCCCTC36640.26390868644205134No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT35540.2559856636503959No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT34750.2502954927363888No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG30800.22184463816635322No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT29350.21140065357735283No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC28720.206862922342132No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG28030.20189302622736627No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC28010.20174897126751795No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC27530.19829165223115924No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC26980.1943301408353315No Hit
GATTATTACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGG26850.19339378359631768No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG26790.19296161871677284No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT26370.1899364645599589No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTCGCTTTGTTTGGAGGGTGTGGTGGTCTCCA26270.1892161897607175No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC26080.18784766764215885No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25350.18258966160769657No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC25350.18258966160769657No Hit
GTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG25210.1815812768887586No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG24990.17999667233042752No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA24800.1786281502118688No Hit
GTCTATTACTGTGCGCGAGGGTTTACCAGTGGTAAATTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCGC24760.17834004029217226No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTCGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA24460.17617921589444804No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGACTCCAGT23840.17171351213915131No Hit
CCCCTGAGGGCCGCTGATTATTACTATAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTTAA23690.17063309994028922No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA23340.1681121381429443No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA23200.16710375342400632No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT23190.1670317259440822No Hit
CAGTAATAGACAGCCGTGTCCTCGGCTCTCAGGCTGCTCATTTGCAGATA22650.1631422420281786No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG22390.16126952755015092No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC22330.16083736267060608No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22120.15932478559219915No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGG21990.15838842835318528No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC21130.1521940650797092No Hit
CCACCACACCCTCCAAACAAAGCGACAACAAGTACGCGGCCAGCAGCTAT21040.15154581776039194No Hit
CTCTTGTTCTGGAAGCAGCTCCAACATCGGAAGTAATTCTGTTAACTGGTACCAGCAGCTCCCAGGAACGGCCCC20840.15010526816190914No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC19590.14110183317139155No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGCTTGGTC18690.1346193599782189No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC18530.13346692029943263No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGACTCCAGTCTGAGGATG18340.132098398180874No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC18330.1320263707009498No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT18250.1314501508615567No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA18180.13094595850208773No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC17860.12864107914451522No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGC17420.125471870027853No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC16940.12201455099149427No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC16940.12201455099149427No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA16890.12165441359187357No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT16790.12093413879263215No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC16490.11877331439490794No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT16400.11812506707559067No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG16130.11618032511763889No Hit
GTGGACTCCAGTCTGAGGATGAGTCTCTTTATTACTGTGCATCATGGGAT15860.11423558315968708No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA15830.11401950071991465No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG15820.11394747323999052No Hit
GTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTTAACAGAATTACTT15790.1137313908002181No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC15600.11236286868165943No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC15510.11171462136234216No Hit
GTCTGAGGATGAGTCTCTTTATTACTGTGCATCATGGGATGACAGCCTGA15460.11135448396272145No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG15170.10926568704492139No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA15010.10811324736613513No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT14650.10552025808886606No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC14460.1041517359703074No Hit
GCTTGGTCCCTCCGCCGAACACCACAGAACCATTCAGGCTGTCATCCCATGATGCACAGTAATAAAGAGACTCAT14450.10407970849038324No Hit
GGCCATCAGTGGACTCCAGTCTGAGGATGAGTCTCTTTATTACTGTGCATCATGGGATGACAGCCTGAATGGTT14260.10271118637182458No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC14200.10227902149227974No Hit
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGT14100.10155874669303833No Hit
TTATTACTGTGCATCATGGGATGACAGCCTGAATGGTTCTGTGGTGTTCGGCGGAGGGACCAAGCTGACCGTC13930.10033427953432794No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)