FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686782

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686782
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1583552
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAACAGTTATCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAGCGAACTGTGGCTGCACCAT71080.44886432526371095No Hit
CTGTTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC68790.43440316453138256No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG55570.35091995715960067No Hit
GAGTATTAGTGGTTGGCTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCTAACCTCCTGATCTATGATGTCTC54300.34290001212464133No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT51400.32458675180859237No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC42370.267563048134826No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC39320.24830255021622277No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC37570.23725144485308977No Hit
GTATTAGTGGTTGGCTGGCCTGGTATCAGCAGAAACCAGGGAAAGCCCCT33620.21230752131916097No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA30390.19191033827749263No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT29180.18426928828355493No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG28840.18212221638443196No Hit
CAGTATAACAGTTATCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAGCGAACTGTGGCTGCACCAT27880.17605989572808473No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG27820.175681000687063No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG27720.17504950895202684No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG27350.172712989532393No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAGTATAACAGTTAT27350.172712989532393No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26990.17043961928626278No Hit
CTAATACTCTGACTGGCCCGGCAAGCGATGGTGACTCTGTCTCCTACAGA25960.1639352544153902No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25600.16166188416926No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT25150.1588201713615972No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT24820.15673624863597785No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG24310.15351564078729338No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22310.14088580608656995No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21670.13684425898233846No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC21520.13589702137978418No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA21430.1353286788182516No Hit
GTTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGC21390.13507608212423716No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCGCTTGCCGGGCCAGTCAGA21140.13349735278664673No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT21110.1333079052661359No Hit
GTAGGAGACAGAGTCACCATCGCTTGCCGGGCCAGTCAGAGTATTAGTGGTTGGCTGGCCTGGTATCAGCAGAAA21040.13286586105161055No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT20590.13002414824394778No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCGCTTGCCGGGCCAGTCAGAGTATTAGTGGTTGG20480.12932950733540802No Hit
GTGCAGCCACAGTTCGCTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC20120.12705613708927777No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC19990.12623519783373074No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATTTCCACCTTGGTCCCTTGGCCGAACGTCCAAGGATAA19920.12579315361920543No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC19890.12560370609869456No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA19220.12137271147395223No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT18910.11941508709534009No Hit
GGATAACTGTTATACTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTG18690.11802580527826051No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG18580.11733116436972073No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC18460.11657337428767733No Hit
GTAATACACAGCCGTGTCCTCATCTCTCAGACTGTTCATTTGCAGATACA18310.11562613668512306No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17810.1124686780099422No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG17750.1120897829689205No Hit
CCACTAATACTCTGACTGGCCCGGCAAGCGATGGTGACTCTGTCTCCTACAGATGCAGACAGGGTGGAAGGAGA17580.111016247019359No Hit
CAGTTATCCTTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAGCGAACTGTGGCTGCACCATCTGTCTTCAT17010.10741674412965284No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16700.1054591197510407No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA16670.10526967223052985No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT16660.10520652305702624No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16290.10287000363739239No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC16210.10236481024936345No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG15910.10047033504425494No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT15910.10047033504425494No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT6550.040.318241
GTATCAA52000.039.521521
TATCAAC61100.033.5107352
ATCAACG62900.032.4246833
TCAACGC63100.032.3168034
AACGCAG63900.032.0160376
CAACGCA63850.031.8822255
ACGCAGA67200.030.39357
CGCAGAG67100.030.287618
AGAGTAC68400.029.66065611
GCAGAGT69850.029.1426759
CGGGTAT1700.027.86900317
TTAACGG1005.4483826E-727.3423635
GACAGGT4350.027.2412511
TCTGGAG10500.026.32454570
TGTTAGA19850.026.06441170
GATTAGC659.686884E-426.0331342
GTAATAC7200.025.8629741
AAAACCG1659.8195225E-525.77228270
CGTTCAT1101.0513486E-625.35853847
Produced by FastQC (version 0.11.5)