FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686785

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686785
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1369983
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT49140.35869058229189704No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC40970.2990548057895609No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAAGACGGTGACCCTGGTCCCAA39070.2851860205564595No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC37680.27503990925434846No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC35770.26109813041475693No Hit
GACCTAAGACGGTGACCCTGGTCCCAACTCCGAAGACGTATCGGCCGTTGCCTAGATACGAAGTACAATAATAAT35570.2596382582849568No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG35060.25591558435396644No Hit
GTCTTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC33660.24569647944536538No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACAGGAACTGG33100.24160883748192497No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC31790.23204667503173398No Hit
TTATTGTACTTCGTATCTAGGCAACGGCCGATACGTCTTCGGAGTTGGGACCAGGGTCACCGTCTTAGGTCAGCC30430.22211954454909294No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC29750.21715597930777242No Hit
GTACAATAATAATCAGCCTCATCATCAGCCTGGAGCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAG29080.2122654076729419No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT28540.20832375292248154No Hit
GCCTTGGGCTGACCTAAGACGGTGACCCTGGTCCCAACTCCGAAGACGTATCGGCCGTTGCCTAGATACGAAGTA27090.19773967998143044No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT26380.1925571339206399No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT25530.18635267736898925No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAAGACGGTGACCCTGGTC24750.18065917606276866No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT24700.18029420803031862No Hit
TCTCTGGGCTCCAGGCTGATGATGAGGCTGATTATTATTGTACTTCGTAT24100.1759145916409182No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG23540.17182694967747775No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC23220.1694911542697975No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC22940.1674473332880773No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACAGGAACTGGCGGTGATGTT22500.16423561460251695No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG21420.15635230510159615No Hit
GTCAGTCACCATCTCCTGCACAGGAACTGGCGGTGATGTTGGTTATTATAACCATGTCTCCTGGTACCAACAACA21150.15438147772636593No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG21020.15343256084199586No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG20320.14832300838769533No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG19850.14489230888266497No Hit
GATTATTATTGTACTTCGTATCTAGGCAACGGCCGATACGTCTTCGGAGTTGGGACCAGGGTCACCGTCTTAGGT19610.1431404623269048No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC19230.1403667052802845No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA18890.13788492265962424No Hit
CTCCTGCACAGGAACTGGCGGTGATGTTGGTTATTATAACCATGTCTCCTGGTACCAACAACACCCAGGCAAAGC18790.13715498659472417No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG17380.1268628880796331No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGC17230.12576798398228298No Hit
GTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGAC17010.12416212463950284No Hit
GACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCT16900.12335919496811273No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA16870.12314021414864271No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGT16740.12219129726427262No Hit
GCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCA16000.11678977038401207No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT15870.11584085349964196No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC15550.11350505809196172No Hit
CTCAGGACGTCACCACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTC15470.11292110924004167No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC15450.11277512202706166No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG15450.11277512202706166No Hit
GCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGACCCCTGCGGGCCGGT15440.11270212842057165No Hit
TCTTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG15330.11189919874918156No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC15300.11168021792971154No Hit
GCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC15200.11095028186481146No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA15090.11014735219342137No Hit
ACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACT14680.10715461432733107No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA14570.10635168465594098No Hit
CCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGG14460.10554875498455091No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC14260.10408888285475075No Hit
CCCTGACCGTCTCTGGGCTCCAGGCTGATGATGAGGCTGATTATTATTGT14220.10379690842879073No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGG14100.10292098515091064No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT13710.10007423449780034No Hit
ATAATAATCAGCCTCATCATCAGCCTGGAGCCCAGAGACGGTCAGGGAGG13700.10000124089131032No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)