FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686786

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686786
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1369983
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG63160.4610276185908876No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT42300.30876295545273186No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC41050.29963875464148093No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC35670.26036819434985686No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAAGACGGTGACCCTGGTCCCAA34480.251681955177546No Hit
GTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCACAGGAACTGG32880.24000297813914478No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC32700.23868909322232465No Hit
GACCTAAGACGGTGACCCTGGTCCCAACTCCGAAGACGTATCGGCCGTTGCCTAGATACGAAGTACAATAATAAT32410.23657227863411442No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG32180.23489342568484428No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC30950.2259152120865733No Hit
TTATTGTACTTCGTATCTAGGCAACGGCCGATACGTCTTCGGAGTTGGGACCAGGGTCACCGTCTTAGGTCAGCC28520.2081777657095015No Hit
GTCTTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC27860.20336018768116101No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC26270.19175420424924983No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT25820.18846949195719948No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG25560.18657165818845928No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT25020.18263000343799887No Hit
GCCTTGGGCTGACCTAAGACGGTGACCCTGGTCCCAACTCCGAAGACGTATCGGCCGTTGCCTAGATACGAAGTA24440.17839637426157845No Hit
GTACAATAATAATCAGCCTCATCATCAGCCTGGAGCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTGGAG24260.1770824893447583No Hit
TCTCTGGGCTCCAGGCTGATGATGAGGCTGATTATTATTGTACTTCGTAT23270.16985612230224753No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAAGACGGTGACCCTGGTC23090.16854223738542742No Hit
GGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC22990.16781230132052732No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT22670.1654765059128471No Hit
CTCCTGGACAGTCAGTCACCATCTCCTGCACAGGAACTGGCGGTGATGTT21930.16007497903258652No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC20590.15029383576292554No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG20470.14941791248504543No Hit
GTCAGTCACCATCTCCTGCACAGGAACTGGCGGTGATGTTGGTTATTATAACCATGTCTCCTGGTACCAACAACA20050.1463521810124651No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG20040.14627918740597512No Hit
GATTATTATTGTACTTCGTATCTAGGCAACGGCCGATACGTCTTCGGAGTTGGGACCAGGGTCACCGTCTTAGGT19420.14175358380359462No Hit
ATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTG19230.1403667052802845No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG18900.13795791626611426No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA18530.13525715282598397No Hit
CTCCTGCACAGGAACTGGCGGTGATGTTGGTTATTATAACCATGTCTCCTGGTACCAACAACACCCAGGCAAAGC17980.13124250446903354No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC17020.12423511824599283No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC16660.12160734841235256No Hit
GGCCTGGGCTCTGCTCCTCCTCACCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGC16020.11693575759699208No Hit
GCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCTCCTGCA15560.11357805169845174No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA15290.11160722432322152No Hit
GACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCACCATCT15170.11073130104534143No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC15060.10992837137395137No Hit
GTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCAGAGAAGCGATCAGGGAC14990.10941741612852131No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC14890.10868748006362124No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT14880.10861448645713122No Hit
TCCTCACCCTCCTCACTCAGGGCACAGGATCCTGGGCTCAGTCTGCCCTGACTCAGCCTCGCTCAGTGTCCGGGT14710.10737359514680109No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG14650.10693563350786105No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT14590.10649767186892099No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA14400.10511079334561085No Hit
GCCCTGACTCAGCCTCGCTCAGTGTCCGGGTCTCCTGGACAGTCAGTCAC14350.10474582531316082No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA14220.10379690842879073No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC14070.10270200433144061No Hit
CTCAGGACGTCACCACCATGGCCTGGGCTCTGCTCCTCCTCACCCTCCTC14050.1025560171184606No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT14030.10241002990548059No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC13790.1006581833497204No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19500.040.406161
GTGGTAT6000.037.358281
GTATCAA44950.036.0398451
TGGTATC6600.033.950912
TATCAAC49500.032.167632
ATCAACG51200.031.221923
TCAACGC52150.030.7103944
CAACGCA53300.030.1729035
AACGCAG53900.029.8370276
GCTTGAC953.676978E-728.599641
ACGCAGA57600.027.9793197
CGCAGAG58000.027.961858
AGAGTAC59800.026.94899211
CAGAGTA60550.026.6712210
GCAGAGT60650.026.6272499
AATGGTA6150.025.59320470
GACAGGT2150.025.2741011
AGGTACG700.001464662624.250652
GTGCTAT1001.7662609E-523.773451
GGATATT9550.023.1155471
Produced by FastQC (version 0.11.5)