FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686791

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686791
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1331702
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCTAGACTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATAT55120.4139064144981385No Hit
GACTTAGGACGGTCAACTTGGTCCCTCCGCCGAATACCAGAGTGCTTCTGCTTGTATATGAGCTGCAGTAATAAT54890.4121793013752326No Hit
GTCTAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCATAGAAG41830.3141093127441425No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG41110.308702697750698No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG37910.28467329777983363No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACTCGTAGTGACGTT37590.28227035778274717No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT34730.2607940815587872No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC32850.2466768090759044No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAACTTGGTCCCTC32390.24322258283009263No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG31710.23811633533628393No Hit
GATTATTACTGCAGCTCATATACAAGCAGAAGCACTCTGGTATTCGGCGGAGGGACCAAGTTGACCGTCCTAAGT31410.23586357908901542No Hit
GCCTTGGGCTGACTTAGGACGGTCAACTTGGTCCCTCCGCCGAATACCAGAGTGCTTCTGCTTGTATATGAGCTG30330.22775365659884872No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT29700.22302286847958475No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC29340.22031956098286254No Hit
GTAATAGACGGCCGCGTCCTCGACTCTCAGGCTGTCCATTTGCAGAAATA28100.2110081684941526No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT27940.20980669849560937No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC27030.20297333787889482No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG26040.19553924226290864No Hit
CTCCTGCACTGGAACTCGTAGTGACGTTGGTGCTTATAACCATGTCTCCTGGTACCAGCAGCACCCAGGCGAAGC25950.19486341538872812No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG25800.19373703726509384No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG25800.19373703726509384No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT25710.19306121039091328No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC25690.19291102664109538No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGTCTAGAGATGGTCAGGGAGG24140.18127178603020794No Hit
GTCGATCACCATCTCCTGCACTGGAACTCGTAGTGACGTTGGTGCTTATAACCATGTCTCCTGGTACCAGCAGCA23650.17759228415966935No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA23140.17376259853931283No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA22570.16948236166950265No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT22200.16670396229787146No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC22050.16557758417423718No Hit
GCTTATAACCATGTCTCCTGGTACCAGCAGCACCCAGGCGAAGCCCCCAAACTCATGATTTATGAGGTCAGCAAT21930.16467648167532975No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG21290.15987060168115688No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT20830.15641637543534515No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT20400.15318742481426026No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA20090.15085957669208275No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAACTTGGTCCCTCCGCCGAATAC19960.1498833823182664No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC19650.1475555341960889No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19100.1434254810760966No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC19100.1434254810760966No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG18520.13907015233137743No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC18280.1372679473335626No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18030.1353906504608388No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA17200.1291580248433959No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17140.1287074735939422No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC16430.12337595047540666No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC15890.1193209892303233No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15780.11849497860632484No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA15730.11811951923178009No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG15500.11639240610887422No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAACTTGGTCCCTCCGCCGAATACCAGAGTGCTT15400.1156414873597847No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT15310.11496566048560414No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC14700.11038505611615812No Hit
GTATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGTCTAGA14530.10910849424270595No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC14470.10865794299325224No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT14260.10708101362016428No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT14050.1055040842470763No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13780.10347660362453462No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC13630.10235022550090034No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG13570.10189967425144665No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC13530.10159930675181085No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC13350.10024765300344972No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)