FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686792

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686792
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1331702
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCTAGACTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATAT51340.38552168578255497No Hit
GACTTAGGACGGTCAACTTGGTCCCTCCGCCGAATACCAGAGTGCTTCTGCTTGTATATGAGCTGCAGTAATAAT45800.34392078708299606No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG42880.3219939596095823No Hit
GTCTAGAGATGGTCAGGGAGGCCGTGTTGCCAGACTTGGAGCCATAGAAG38950.29248285277036457No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG36840.27663846716457585No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAACTCGTAGTGACGTT35340.26537468592823316No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG31310.23511266033992592No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT30800.2312829747195694No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC29840.22407415472831008No Hit
GATTATTACTGCAGCTCATATACAAGCAGAAGCACTCTGGTATTCGGCGGAGGGACCAAGTTGACCGTCCTAAGT29260.21971882598359094No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC28410.2133360166163301No Hit
GGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC27680.20785430974797664No Hit
GCCTTGGGCTGACTTAGGACGGTCAACTTGGTCCCTCCGCCGAATACCAGAGTGCTTCTGCTTGTATATGAGCTG27640.20755394224834084No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAACTTGGTCCCTC27240.20455026725198278No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG27190.20417480787743805No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG26820.20139640850580687No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT26550.19936892788326518No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT25280.1898322597698284No Hit
CTCCTGCACTGGAACTCGTAGTGACGTTGGTGCTTATAACCATGTCTCCTGGTACCAGCAGCACCCAGGCGAAGC24730.18570220664983608No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT24250.18209779665420642No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC23880.17931939728257523No Hit
GTCGATCACCATCTCCTGCACTGGAACTCGTAGTGACGTTGGTGCTTATAACCATGTCTCCTGGTACCAGCAGCA23840.17901902978293943No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAACTTGGTCCCTCCGCCGAATAC23830.17894393790803048No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG23440.1760153547865814No Hit
GTAATAGACGGCCGCGTCCTCGACTCTCAGGCTGTCCATTTGCAGAAATA23070.1732369554149502No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC23060.17316186354004126No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22710.17053364791822795No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT22310.16752997292186989No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG22260.16715451354732516No Hit
GTAATAATCAGCCTCGTCCTCAGCCTGGAGTCTAGAGATGGTCAGGGAGG21160.15889440730734053No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA20840.1564914673102541No Hit
GCTTATAACCATGTCTCCTGGTACCAGCAGCACCCAGGCGAAGCCCCCAAACTCATGATTTATGAGGTCAGCAAT20570.1544639866877124No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA20140.15123503606662753No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG20060.1506343010673559No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA20040.15048411731753802No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18440.13846941733210583No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC18070.13569101796047464No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17920.1345646398368404No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC17630.13238697546448078No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTC17160.1288576573437601No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC17060.1281067385946706No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT16930.1271305442208542No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC16080.12074773485359336No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA15830.11887043798086959No Hit
GTCTATTACTGTGCGAGAGACACACATTATGATTTTTGGAGTGGGCTCGA15620.11729350860778162No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA15460.11609203860923839No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCG15360.1153411198601489No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC15110.11346382298742512No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG15060.11308836361288035No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC15000.11263781236342665No Hit
TCCTCACTCAGGGCACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCT14590.10955904549215965No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA14340.10768174861943587No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT14320.10753156486961797No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC13950.10475316549798679No Hit
GTATATGAGCTGCAGTAATAATCAGCCTCGTCCTCAGCCTGGAGTCTAGA13730.10310114424998985No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC13660.10257550112562722No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT13590.10204985800126454No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAACTTGGTCCCTCCGCCGAATACCAGAGTGCTT13560.1018245823765377No Hit
GTGTGACCTGGAGCGAAAGCGGACAGAACGTGACCGCCAGAAACTTCCCACCTAGCCAGGATGCCTCCGGGGAC13440.10092347987763028No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC13340.10017256112854077No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGGAG9450.049.3982170
TGGTATC3500.045.6163062
GTGGTAT3550.044.994111
TAGACGG6750.042.7572485
ATAGACG6900.041.8293154
GCGTCCT7250.039.3563914
GGTATCA10450.039.3509521
GTAATAG7500.038.9693641
GACGGCC7350.038.8048977
CGACTCT7450.038.768921
TAATAGA7500.038.4988672
CTCGACT7600.038.44360419
GTATCAA26200.038.3951641
CGCGTCC7550.038.2410413
GACTCTC7650.037.752522
AATAGAC7800.037.0042463
CCGCGTC7800.036.56752812
TCGACTC7900.036.55642320
ACTCTCA8450.034.1821523
TATCAAC29250.034.027662
Produced by FastQC (version 0.11.5)