FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686795

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686795
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences928469
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGTCAGCCTCATCTTCAGATTGGAGCCCAGAGATGAGTAAAAGGC50070.5392748707819001No Hit
GACTATTACTGTGCCATCTGGTACAGCACTTCTGTGGTTTTCGGCGGAGGGACCAACCTGACCGTCCTAGGTCAG42130.45375774527744056No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC33050.3559623423076053No Hit
GCCTTGGGCTGACCTAGGACGGTCAGGTTGGTCCCTCCGCCGAAAACCAC32760.352838920847115No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT28700.3091110204002503No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG27120.2920937586499926No Hit
GACCTAGGACGGTCAGGTTGGTCCCTCCGCCGAAAACCACAGAAGTGCTGTACCAGATGGCACAGTAATAGTCAG26560.28606232410559745No Hit
GGATATACTGGTACCAGCAGAAGCCAGGGAGTCTTCCCCGGTATCTCCTG25470.27432256758168555No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGGTTGGTCCCTC24870.2678603162841193No Hit
CACCTGCACCTTGCGCAATGACATCAATGTTGGTACCTACAGGATATACT24150.2601056147270399No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT23190.24976601265093396No Hit
GTGCTGACTCAGCCAACCTCCCTCTCAGCATCTCCTGGAACATCAGTCAG20740.2233784865192053No Hit
GGTTCCCTCTCGCAGCCTGTGCTGACTCAGCCAACCTCCCTCTCAGCATCTCCTGGAACATCAGTCAGATTCAC20020.21562378496212584No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19880.21411592632602705No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC19320.2080844917816319No Hit
CTACAGGATATACTGGTACCAGCAGAAGCCAGGGAGTCTTCCCCGGTATC19050.20517647869772712No Hit
GTACCAGATGGCACAGTAATAGTCAGCCTCATCTTCAGATTGGAGCCCAG18850.2030223949318717No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG18790.20237616980211512No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17670.19031330071332483No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17450.18794380857088389No Hit
CTCCAGAGCCCTGTTGCCTATCTGAGTCTGATTTGTAATTCAGGAGATACCGGGGAAGACTCCCTGGCTTCTG17440.18783610438259113No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG17350.18686676668795618No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17260.18589742899332126No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG16660.17943517769575507No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT16570.17846584000112012No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA16550.17825043162453458No Hit
ATCTGGTACAGCACTTCTGTGGTTTTCGGCGGAGGGACCAACCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC16300.17555782691721533No Hit
GGGTAAGAGGCTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGCTCCTCTCTCACTGCACAGGTTCCCT15980.17211129289184668No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG15950.17178818032696838No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA15930.17157277195038284No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC15910.1713573635737973No Hit
CTCGCAGCCTGTGCTGACTCAGCCAACCTCCCTCTCAGCATCTCCTGGAA15730.16941868818452743No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15180.16349495782842507No Hit
CAGTAATAGTCAGCCTCATCTTCAGATTGGAGCCCAGAGATGAGTAAAAG14820.15961760704988534No Hit
GCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTGCAACCAATGCAGGCCTTTTACTCATCTCTGGG14670.1580020442254938No Hit
CCTCTCCTCCTCCTGCTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGCCTGTGCTGACTCAGCCAACCTCCCT14630.15757122747232272No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14580.15703270653085888No Hit
ATCCTGTAGGTACCAACATTGATGTCATTGCGCAAGGTGCAGGTGAATCT14420.15530943951817455No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT14220.15315535575231914No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG14190.15283224318744085No Hit
GGCCTGGACTCCTCTCCTCCTCCTGCTCCTCTCTCACTGCACAGGTTCCC14190.15283224318744085No Hit
TCTCTGGGCTCCAATCTGAAGATGAGGCTGACTATTACTGTGCCATCTGG14100.1518629054928059No Hit
GTACGGGACTGTGGGGTAAGAGGCTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGCTCCTCTCTCACTG14090.15175520130451314No Hit
GTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGCTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGCCTGTG14030.15110897617475652No Hit
CTATTACTGTGCCATCTGGTACAGCACTTCTGTGGTTTTCGGCGGAGGGACCAACCTGACCGTCCTAGGTCAGCC13900.1497088217269505No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA13850.14917030078548665No Hit
GTAAGAGGCTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGCTCC13750.14809325890255895No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGG13230.14249264111133492No Hit
GTGCAGGTGAATCTGACTGATGTTCCAGGAGATGCTGAGAGGGAGGTTGG13130.1414155992284072No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC13120.14130789504011443No Hit
CTCACTGCACAGGTTCCCTCTCGCAGCCTGTGCTGACTCAGCCAACCTCC12970.13969233221572286No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12930.1392615154625518No Hit
GGTTGGTCCCTCCGCCGAAAACCACAGAAGTGCTGTACCAGATGGCACAG12860.1385075861445024No Hit
GTATATCCTGTAGGTACCAACATTGATGTCATTGCGCAAGGTGCAGGTGAATCTGACTGATGTTCCAGGAGATG12670.13646120656693977No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGGTTGGTCCCTCCGCCGAAAACCACAGAAGTG12410.13366089767132774No Hit
GCACAGGTTCCCTCTCGCAGCCTGTGCTGACTCAGCCAACCTCCCTCTCA12320.1326915599766928No Hit
GTGCTGTACCAGATGGCACAGTAATAGTCAGCCTCATCTTCAGATTGGAG12220.1316145180937651No Hit
CAGATAGGCAACAGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAA12100.13032206783425188No Hit
ATGTTGGTACCTACAGGATATACTGGTACCAGCAGAAGCCAGGGAGTCTTCCCCGGTATCTCCTGAATTACAAAT11970.12892191338644587No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC11700.12601390030254106No Hit
ACCTAGGACGGTCAGGTTGGTCCCTCCGCCGAAAACCACAGAAGTGCTGTACCAGATGGCACAGTAATAGTCAGC11560.12450604166644229No Hit
GTACAGCACTTCTGTGGTTTTCGGCGGAGGGACCAACCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGT11330.12202884533570857No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11200.12062869088790257No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC11130.11987476156985316No Hit
CCTCTCTCACTGCACAGGTTCCCTCTCGCAGCCTGTGCTGACTCAGCCAA11110.11965935319326762No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10990.11836690293375439No Hit
GTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGCTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGCCTGTG10980.1182591987454616No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC10890.11728986105082669No Hit
GGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTGCAACCA10730.11556659403814237No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG10590.11405873540204357No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT10390.11190465163618817No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT10250.1103967930000894No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT10070.10845811761081954No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC9850.10608862546837858No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG9850.10608862546837858No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG9610.1035037249493521No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC9550.1028574998195955No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC9550.1028574998195955No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT9450.10178045793666779No Hit
CATCAATGTTGGTACCTACAGGATATACTGGTACCAGCAGAAGCCAGGGAGTCTTCCCCGGTATCTCCTGAATTA9410.1013496411834967No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT9310.10027259930056899No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9310.10027259930056899No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG2750.055.8974570
TAACGTG250.006778415852.83481269
CATTATC200.00764844351.2467530
GGTATCA6800.045.120281
CATGAAT658.7943557E-442.99803570
GTATCAA19500.039.335631
GTGGTAT1850.038.6977541
CGTATGC3100.037.8900841
CTTATAC3250.037.7622071
TATACAC3250.037.7622073
TCGTATG2950.037.43162540
ATCTCGT2500.037.12211637
TCTCCGA3150.036.78461511
ACCGTAC2900.036.46751427
TCTCGTA2600.035.72733338
GACCGTA3000.035.23863226
ATCTCCG3300.035.11258710
GCCGTCT3000.034.8090846
GTATGCC3300.034.5940542
ACTAGAT2900.034.2125232
Produced by FastQC (version 0.11.5)