FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686796

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686796
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences928469
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGTCAGCCTCATCTTCAGATTGGAGCCCAGAGATGAGTAAAAGGC44620.4805760881623404No Hit
GACTATTACTGTGCCATCTGGTACAGCACTTCTGTGGTTTTCGGCGGAGGGACCAACCTGACCGTCCTAGGTCAG37660.4056139731105723No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC29510.3178350596519647No Hit
GCCTTGGGCTGACCTAGGACGGTCAGGTTGGTCCCTCCGCCGAAAACCAC29440.3170811303339153No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29270.3152501591329382No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG28120.30286417747926964No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT25700.2767997639124193No Hit
GGATATACTGGTACCAGCAGAAGCCAGGGAGTCTTCCCCGGTATCTCCTG23830.25665908070167126No Hit
GACCTAGGACGGTCAGGTTGGTCCCTCCGCCGAAAACCACAGAAGTGCTGTACCAGATGGCACAGTAATAGTCAG23190.24976601265093396No Hit
GTGCTGACTCAGCCAACCTCCCTCTCAGCATCTCCTGGAACATCAGTCAG22060.23759543937385091No Hit
CACCTGCACCTTGCGCAATGACATCAATGTTGGTACCTACAGGATATACT21900.23587217236116662No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGGTTGGTCCCTC20690.22283996557774144No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT20150.21702393940993184No Hit
GGTTCCCTCTCGCAGCCTGTGCTGACTCAGCCAACCTCCCTCTCAGCATCTCCTGGAACATCAGTCAGATTCAC19240.20722285827528975No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18840.20291469074357896No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT18580.20011438184796693No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC17620.189774779771861No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG17590.18945166720698267No Hit
CTACAGGATATACTGGTACCAGCAGAAGCCAGGGAGTCTTCCCCGGTATC17130.18449727454551526No Hit
CTCCAGAGCCCTGTTGCCTATCTGAGTCTGATTTGTAATTCAGGAGATACCGGGGAAGACTCCCTGGCTTCTG16860.18158926146161045No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16860.18158926146161045No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC16350.1760963478586792No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG16250.1750193059757515No Hit
GTACCAGATGGCACAGTAATAGTCAGCCTCATCTTCAGATTGGAGCCCAG15940.1716804761386756No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG15820.17038802587916235No Hit
GGGTAAGAGGCTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGCTCCTCTCTCACTGCACAGGTTCCCT14890.16037153636793475No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14770.1590790861084215No Hit
ATCTGGTACAGCACTTCTGTGGTTTTCGGCGGAGGGACCAACCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC14740.15875597354354318No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG14590.15714041071915164No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14360.1546632143884179No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA14330.1543401018235396No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC14130.1521860180576842No Hit
GCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTGCAACCAATGCAGGCCTTTTACTCATCTCTGGG14100.1518629054928059No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG14030.15110897617475652No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13970.15046275104499987No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT13900.1497088217269505No Hit
CCTCTCCTCCTCCTGCTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGCCTGTGCTGACTCAGCCAACCTCCCT13820.14884718822060833No Hit
CTCGCAGCCTGTGCTGACTCAGCCAACCTCCCTCTCAGCATCTCCTGGAA13720.14777014633768065No Hit
GTACGGGACTGTGGGGTAAGAGGCTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGCTCCTCTCTCACTG13590.14636999188987462No Hit
TCTCTGGGCTCCAATCTGAAGATGAGGCTGACTATTACTGTGCCATCTGG13260.14281575367621321No Hit
CTATTACTGTGCCATCTGGTACAGCACTTCTGTGGTTTTCGGCGGAGGGACCAACCTGACCGTCCTAGGTCAGCC13100.1410924866635289No Hit
CAGTAATAGTCAGCCTCATCTTCAGATTGGAGCCCAGAGATGAGTAAAAG12790.137753656826453No Hit
GTATATCCTGTAGGTACCAACATTGATGTCATTGCGCAAGGTGCAGGTGAATCTGACTGATGTTCCAGGAGATG12750.13732284007328194No Hit
GGCCTGGACTCCTCTCCTCCTCCTGCTCCTCTCTCACTGCACAGGTTCCC12720.1369997275084036No Hit
GTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGCTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGCCTGTG12480.13441482698937712No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC12300.13247615160010726No Hit
CTCACTGCACAGGTTCCCTCTCGCAGCCTGTGCTGACTCAGCCAACCTCC11910.12827568825668922No Hit
ATCCTGTAGGTACCAACATTGATGTCATTGCGCAAGGTGCAGGTGAATCT11880.12795257569181093No Hit
GGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAAGATGCTGCAACCA11760.12666012543229768No Hit
GGTTGGTCCCTCCGCCGAAAACCACAGAAGTGCTGTACCAGATGGCACAG11730.12633701286741936No Hit
GTGCTGTACCAGATGGCACAGTAATAGTCAGCCTCATCTTCAGATTGGAG11690.1259061961142483No Hit
GTGCAGGTGAATCTGACTGATGTTCCAGGAGATGCTGAGAGGGAGGTTGG11690.1259061961142483No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11640.12536767517278444No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC11620.1251522667961989No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGGTTGGTCCCTCCGCCGAAAACCACAGAAGTG11620.1251522667961989No Hit
GTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGCTCCTCTCTCACTGCACAGGTTCCCTCTCGCAGCCTGTG11550.1243983374781495No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11550.1243983374781495No Hit
GCACAGGTTCCCTCTCGCAGCCTGTGCTGACTCAGCCAACCTCCCTCTCA11420.12299818303034349No Hit
GTAAGAGGCTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTGCTCC11370.12245966208887964No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC11240.12105950764107364No Hit
ATGTTGGTACCTACAGGATATACTGGTACCAGCAGAAGCCAGGGAGTCTTCCCCGGTATCTCCTGAATTACAAAT11240.12105950764107364No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGG11210.12073639507619532No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT11170.12030557832302426No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC11120.1197670573815604No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC11060.11912083225180377No Hit
GTACAGCACTTCTGTGGTTTTCGGCGGAGGGACCAACCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGT10700.11524348147326405No Hit
CAGATAGGCAACAGGGCTCTGGAGTCCCCAGCCGCTTCTCTGGATCCAAA10360.11158153907130987No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG10210.10996597624691831No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT10120.10899663855228339No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT9940.10705796316301353No Hit
GTATCAACGCAGAGTACGGGACTGTGGGGTAAGAGGCTGTGTCCACCATGGCCTGGACTCCTCTCCTCCTCCTG9810.10565780871520751No Hit
CCTCTCTCACTGCACAGGTTCCCTCTCGCAGCCTGTGCTGACTCAGCCAA9760.10511928777374366No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT9510.1024266830664244No Hit
CTGCACAGGTTCCCTCTCGCAGCCTGTGCTGACTCAGCCAACCTCCCTCT9330.10048800767715454No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTAA300.001888532772.9455870
CGTGCGT400.005903729254.70918370
TAGTCGT200.00689515852.60825352
GGTATCA8750.042.0613141
GCTTGTA759.840733E-1040.8929441
GTATCAA24300.040.38811
CTACCGT350.001249769640.03804451
GTGGTAT2400.036.9172441
TATCAAC27950.035.098662
TGGTATC2550.034.730662
TTTGGAT17650.034.3030270
ATCAACG29400.033.478053
TCAACGC29750.032.9590574
CAACGCA30250.032.4090425
AACGCAG30300.032.2414786
AGAGTAC32100.031.17414911
TAACACG553.519396E-430.9511364
CGCAGAG31800.030.8276968
GCAGAGT32650.030.1293899
CAGAGTA32800.030.09537910
Produced by FastQC (version 0.11.5)