FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686797

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686797
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences490413
Sequences flagged as poor quality0
Sequence length20-76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT35140.7165389171983614No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT11280.2300102158792691No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC10540.2149208932063383No Hit
CCCTTGGACAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAAAGCCTCGTA8400.17128420331434935No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG8210.16740991776319142No Hit
GTACACACTGGCCCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGA7830.15966134666087564No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC7820.15945743689502523No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCC7710.15721442947067066No Hit
GTGTGTACCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCC7670.15639879040726898No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7600.1549714220463161No Hit
GTAATACACAGCCATGTCCTCAGCTCTCAGGCTGCCCATTTGAAGATACA7200.14681503141229943No Hit
GGTACACACTGGCCCACCTTCGGCCAAGGGACACGACTGGAGATTAAACG7010.14294074586114153No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG6880.1402899189050861No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG6190.1262201450614074No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA6050.1233654083395016No Hit
GTCTGGGACCCCAGAGCCCCGGTTAGAAACCTTATAAATTAGGCGCCTTGGAGATTGGCCTGGCCTCTGCTGAAA5780.11785984466154038No Hit
GTCCCAGACAGATTCAGCGGCAGTGGGTCAGGCACTGATTTCACACTGAA5770.11765593489568996No Hit
CGTATACAGTGATGGAAACACCTACTTGAATTGGTTTCAGCAGAGGCCAG5600.1141894688762329No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTGATTTTCAGTGTGAAATCAGTGCCTGACCCACTGCCG5420.1105190930909254No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC5410.11031518332507498No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT5410.11031518332507498No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT5290.10786826613487001No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT5280.10766435636901958No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC5160.10521743917881458No Hit
GGCCAGGCCAATCTCCAAGGCGCCTAATTTATAAGGTTTCTAACCGGGGC5130.10460570988126335No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG5030.10256661222275919No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG4960.10113924386180627No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATCGT150.0026640566.885210
GTCGTCT200.00661380453.16126349
TCGCGTT200.00749382751.50832438
ATGTCGT408.681345E-751.37386337
TGTCGTA451.9200033E-645.78517538
CTTATAC9250.044.483221
ATCGCGT551.5539445E-743.58994737
AATCGCG551.5809383E-743.4945736
TATACAC9750.043.231353
TTATACA9550.043.0858422
CTAATCG804.0017767E-1142.5538434
ATGCCGT8450.042.16560444
GTCGTAT503.845278E-641.38864539
CCGTCTT7850.041.29482347
CGTATGC8900.041.2826441
GGTATCA9100.041.1727141
TGCCGTC8450.040.6245445
TCGTATG8850.039.85237540
GCCGTCT8300.039.77680246
TGCTTGA7800.039.69775455
Produced by FastQC (version 0.11.5)