FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686801

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686801
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1997008
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT62710.31401977358127764No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC61760.3092626569347744No Hit
CTATAATAGTTATTCTCCGATGTGCAGTTTTGGCCAGGGGACCAAGCTGG57630.2885817182505027No Hit
CTATTATAGTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTC57440.2876302949212021No Hit
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT47940.24005912845617042No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC46840.23455088812864044No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC46790.23430051356829817No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC43610.21837669153052966No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG41530.20796110982029115No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG41200.20630863772203215No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA39940.1999991988014069No Hit
CCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCG39880.19969874932899617No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGAGAATTGGTAGCTGGTTGGCCTGGTTTCAGCAGAAA38160.191085864453222No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG37520.18788107008084093No Hit
GTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGA37190.18622859798258196No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT36400.18227267992917404No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG36220.18137133151194187No Hit
GAATTCACTCTCACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTA35880.17966878450161441No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC35490.1777158629309447No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT35380.1771650388981917No Hit
GTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA34360.17205739786720933No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT31860.15953866985009574No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG30560.15302893128119666No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACAT30290.1516769086553484No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC30060.15052518567777395No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT28480.14261334957095814No Hit
GTTTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGGCCAACCAGCTACCA27980.1401096039675354No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG27890.13965892975891936No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT27720.13880765625375563No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT27010.13525233749689536No Hit
CCTTATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGGCCAACCAGCTACCAATTCTCTGA26960.1350019629365531No Hit
GGCCAAAACTGCACATCGGAGAATAACTATTATAGTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGG26590.13314919119002028No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC26340.1318973183883089No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG25750.12894289857627012No Hit
GACCCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG25670.12854229927972247No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG25620.1282919247193802No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCCCAGGTGCCAAATGTGACATCGAGATGACCCAGTCTCCTTCCACC24990.12513720525906757No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAACTG24500.1226835345677133No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG24320.12178218615048113No Hit
ACCTTGATGGGACCCCACTTTCTAAACTAGACGCCTTATAGATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTG24060.1204802384367013No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23960.11997948931601676No Hit
CACTATAATAGTTATTCTCCGATGTGCAGTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCA23210.1162238709108827No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT22870.11452132390055522No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA22680.11356990057125459No Hit
GTCTCCTTCCACCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTTGCCGGGCGAGTCAGAGAATTGGTAG22620.11326945109884387No Hit
GTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCC22130.11081578040748961No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA22080.11056540584714733No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC21390.10711023691442396No Hit
ATTATAGTGTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGC20930.10480679095927507No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC20470.10250334500412617No Hit
GATCAGGAGTTTAGGGGCTTTCCCTGGTTTCTGCTGAAACCAGGCCAACC20160.10095102273000409No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC20150.10090094781793563No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCGA605.906043E-446.62307470
GGTATCA22900.041.805991
CTTATAC14100.041.5457761
CTAATCG1400.041.21898734
TCGTATG11100.040.01886440
ATCGTAT1550.039.88812639
TATACAC15350.039.0338823
CGCGTAT801.715307E-938.6416239
TTATACA15200.038.5382082
ACGAGAC15500.038.23379522
ATCTCGT9750.038.1923137
CGTATGC11300.037.8913741
GTGGTAT7350.037.345171
CGAGACT15900.037.06786723
ATCGCGT1300.036.79075637
ATGCCGT11500.036.63353744
AATCGCG1400.036.51437436
GCTAATC12550.036.45718433
CACGAGA16350.036.44723521
CTAATCT11050.036.24901234
Produced by FastQC (version 0.11.5)