FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686803

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686803
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1189349
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCACCATGGTAACTCACTGTACACTTTTGGCCAGGGGA76070.6395935927974044No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT60610.5096065158334517No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA55840.4695005418930861No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC49270.41426023816390306No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGTTA48400.40694531209930807No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC39310.330516946665781No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG36870.3100015218409399No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT36440.3063860986136113No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC36400.30604978017385986No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT33370.28057365836268416No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTGGCAGCAGCTACTTAGCCTGGTACC33220.27931246421361605No Hit
GTGTATTACTGTCAGCACCATGGTAACTCACTGTACACTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAA32780.27561296137634955No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31750.2669527615527486No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGACGCCAGTGGCCCTCCT31180.2621602237862898No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGAGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGGCAG30730.2583766413390855No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29670.2494642026856709No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA29360.24685773477759682No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA28880.24282191350057888No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT28840.2424855950608274No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27630.2323119622583447No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGAGAAAGAGCCACCCTCTCCTGC27300.22953733513039484No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCCTGGCCAAAAGTG25580.2150756422210806No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG24960.20986270640493246No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24820.20868559186580224No Hit
TGGTAACTCACTGTACACTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT23990.20170698424095873No Hit
GAGTTACCATGGTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGT23930.20120250658133146No Hit
CTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCA23630.19868011828319526No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCC23530.19783932218381653No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC23490.19750300374406504No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT23410.19683036686456204No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23350.1963258892049348No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21550.18119155941611756No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT21300.17908956916767071No Hit
GTAATACACGGCCGTGTCCGCAGCGGTCACAGAGCTCAGTGTCAAGGAGA21130.17766021579872687No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC20920.17589454399003152No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCACCATGGTAACTCA20860.17539006633040427No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20720.17421295179127405No Hit
GTCTTTGTCTCCAGGAGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA20670.17379255374158467No Hit
GGCCAAAAGTGTACAGTGAGTTACCATGGTGCTGACAGTAATACACTGCA20250.170261210124194No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC19910.1674025033863063No Hit
ATCCAGGAGGGCCACTGGCGTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAG19700.16563683157761094No Hit
GTCAGAGTGTTGGCAGCAGCTACTTAGCCTGGTACCAGCAGACACCTGGC19530.1642074782086671No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA18860.15857414434282957No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA18160.15268857164717842No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGACGCCAGTGGCCCTCCTGGATGCACCATAGA18060.15184777554779968No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17910.15058658139873157No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGTTACCGGAGAAATTGTGTTGACGCAGTCTC17800.14966170568941498No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT17780.14949354646953922No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17680.14865275037016049No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG17620.14814827271053324No Hit
GTACACTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTG17510.14722339700121664No Hit
CTGCTACTCTGGCTCCCAGATGTTACCGGAGAAATTGTGTTGACGCAGTC17500.14713931739127875No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC17490.1470552377813409No Hit
GTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCAC17330.1457099640223349No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA17100.1437761329937638No Hit
CGTCTATGGTGCATCCAGGAGGGCCACTGGCGTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA16470.13847911756767778No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15500.13032339540370402No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT15100.1269602110061891No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC15090.12687613139625123No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15030.12637165373662398No Hit
CACCTGGCCAGGCTCCCAAACTCCTCGTCTATGGTGCATCCAGGAGGGCC14580.12258807128941968No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA14570.12250399167948181No Hit
CTCCCAAACTCCTCGTCTATGGTGCATCCAGGAGGGCCACTGGCGTCCCA14200.11939304611178048No Hit
GTCTCTGGTGGCTCCATCACTCCTCACTACTGGAGCTGGATTCGGCAGCC14180.11922488689190473No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT13790.11594578210432765No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13750.11560946366457615No Hit
CTGTACACTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG13740.11552538405463829No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT13630.11460050834532168No Hit
GTGTTGGCAGCAGCTACTTAGCCTGGTACCAGCAGACACCTGGCCAGGCTCCCAAACTCCTCGTCTATGGTGCAT13630.11460050834532168No Hit
CTGCAGGAGAGGGTGGCTCTTTCTCCTGGAGACAAAGACAGGGTGCCTGG13590.11426418990557019No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC13410.11275075692668847No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC13410.11275075692668847No Hit
ACTTAGCCTGGTACCAGCAGACACCTGGCCAGGCTCCCAAACTCCTCGTC13250.11140548316768248No Hit
GTCTGGGACGCCAGTGGCCCTCCTGGATGCACCATAGACGAGGAGTTTGGGAGCCTGGCCAGGTGTCTGCTGGTA13240.11132140355774461No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA13230.11123732394780675No Hit
CTCCTGGATGCACCATAGACGAGGAGTTTGGGAGCCTGGCCAGGTGTCTGCTGGTACCAGGCTAAGTAGCTGCTG13040.10963981135898714No Hit
GTGTATTACTGTGCGAGAGTCGAGGAGGGGTGGGACCTCCCAGGCAACTA13010.10938757252917353No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC12940.1087990152596084No Hit
GTCCTGGACCTCCTGTGCTAGACCATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC12900.1084626968198569No Hit
CCATAGACGAGGAGTTTGGGAGCCTGGCCAGGTGTCTGCTGGTACCAGGCTAAGTAGCTGCTGCCAACACTCTGA12890.10837861720991904No Hit
AAAGTGTACAGTGAGTTACCATGGTGCTGACAGTAATACACTGCAAAATC12800.10762190072047817No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA12650.10636070657141007No Hit
GTGCTAGACCATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTC12610.10602438813165858No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC12530.1053517512521556No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG12340.103754238663336No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG12300.1034179202235845No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC12250.10299752217389513No Hit
GCCTGGTACCAGCAGACACCTGGCCAGGCTCCCAAACTCCTCGTCTATGGTGCATCCAGGAGGGCCACTGGCGTC12220.10274528334408152No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC12180.10240896490433002No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC12180.10240896490433002No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT12160.10224080568445428No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT12040.10123185036519979No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT12010.10097961153538618No Hit
GTACCAGCAGACACCTGGCCAGGCTCCCAAACTCCTCGTCTATGGTGCATCCAGGAGGGCCACTGGCGTCCCAGA11960.10055921348569681No Hit
GCAGACACCTGGCCAGGCTCCCAAACTCCTCGTCTATGGTGCATCCAGGAGGGCCACTGGCGTCCCAGACAGGTT11910.10013881543600744No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA11910.10013881543600744No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGTAT252.5897048E-455.1025839
CGTAAAT200.006942662452.51793350
GGTATCA9000.048.715131
TCGTATG1250.044.1460240
CGTATGC1350.043.49378641
TCGACGT1650.043.4172229
GTGGTAT2150.042.6817321
GTATCAA25200.039.9215931
GTATGCC1650.039.8251542
CGACGTC1750.039.01323730
CTCGACG1750.038.95565428
GGTAATC653.9760926E-738.68031761
CTTATAC1700.037.985811
GACGTCA1800.037.9503731
CGAGACT1850.036.7708623
TGGTATC2750.035.8411562
CACGAGA1900.035.7866421
TATCAAC28300.035.3083652
ACGAGAC1950.034.870522
TAGGCGC400.002533074234.6740244
Produced by FastQC (version 0.11.5)