FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686804

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686804
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1189349
Sequences flagged as poor quality0
Sequence length20-76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCACCATGGTAACTCACTGTACACTTTTGGCCAGGGGA71200.5986468227576599No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT60310.5070841275353155No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA55170.46386720802724846No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGTTA42380.3563293869167082No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC42200.3548159539378265No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC40230.33825227078006537No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT37230.3130283877987033No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC37200.3127761489688897No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATT34260.2880567436471549No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG33260.2796487826533675No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTGGCAGCAGCTACTTAGCCTGGTACC32790.27569704098628744No Hit
GTGTATTACTGTCAGCACCATGGTAACTCACTGTACACTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAA32640.27443584683721933No Hit
GTCCCAGACCCACTGCCACTGAACCTGTCTGGGACGCCAGTGGCCCTCCT32180.27056818478007716No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30760.2586288801688991No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA28690.24122440091175928No Hit
GTCTCCAGGCACCCTGTCTTTGTCTCCAGGAGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGGCAG28510.23971096793287758No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT28510.23971096793287758No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA28280.23777713690430646No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT28040.2357592262657975No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT27440.2307144496695251No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCCTGGCCAAAAGTG25550.21482340339126696No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25260.21238509470306866No Hit
CTCCAGGCACCCTGTCTTTGTCTCCAGGAGAAAGAGCCACCCTCTCCTGC25000.21019902484468392No Hit
GAGTTACCATGGTGCTGACAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGAGTGAAGT24640.2071721588869205No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG23420.19691444647449993No Hit
CTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCA23220.19523285427574244No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT23170.1948124562260531No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22640.19035623689934578No Hit
TGGTAACTCACTGTACACTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTT22510.18926320197015342No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG22270.18724529133164444No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22020.18514330108319763No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21870.1838821069341295No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG21720.1826209127850614No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT21700.18245275356518567No Hit
GACTTCACTCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCACCATGGTAACTCA20990.17648310125959663No Hit
GTCTTTGTCTCCAGGAGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA20700.17404479257139832No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC20620.17337215569189532No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCC20290.17059752856394547No Hit
GTAATACACGGCCGTGTCCGCAGCGGTCACAGAGCTCAGTGTCAAGGAGA20130.1692522548049395No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT19900.16731842377636844No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG19560.16445971703848072No Hit
ATCCAGGAGGGCCACTGGCGTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCACCATCAG19370.16286220444966112No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGTCTGGGACGCCAGTGGCCCTCCTGGATGCACCATAGA19180.16126469186084152No Hit
GGCCAAAAGTGTACAGTGAGTTACCATGGTGCTGACAGTAATACACTGCA19090.16050797537140066No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA18750.15764926863351297No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC18650.15680847253413424No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT18400.1547064822856874No Hit
GTCAGAGTGTTGGCAGCAGCTACTTAGCCTGGTACCAGCAGACACCTGGC17760.1493253872496635No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT17340.14579404363227277No Hit
CTGCTACTCTGGCTCCCAGATGTTACCGGAGAAATTGTGTTGACGCAGTC17050.14335573494407444No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16910.14217862040494422No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG16880.1419263815751306No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA16360.13755424185836118No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT16150.13578857004966582No Hit
GTACACTTTTGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTG16120.1355363312198522No Hit
GTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACTCTCAC16050.1349477739502871No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16000.1345273759005977No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA15940.13402289824097047No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC15760.13250946526208876No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATGTTACCGGAGAAATTGTGTTGACGCAGTCTC15640.13150050994283427No Hit
CGTCTATGGTGCATCCAGGAGGGCCACTGGCGTCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA15260.1283054847651951No Hit
GTCTGGGACGCCAGTGGCCCTCCTGGATGCACCATAGACGAGGAGTTTGGGAGCCTGGCCAGGTGTCTGCTGGTA14530.12216767323973031No Hit
CACCTGGCCAGGCTCCCAAACTCCTCGTCTATGGTGCATCCAGGAGGGCC14160.11905672767202899No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC14140.11888856845215323No Hit
CTGCAGGAGAGGGTGGCTCTTTCTCCTGGAGACAAAGACAGGGTGCCTGG13760.11569354327451405No Hit
GTGCTAGACCATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTC13410.11275075692668847No Hit
GTCTCTGGTGGCTCCATCACTCCTCACTACTGGAGCTGGATTCGGCAGCC13290.11174180160743398No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC13210.111069164727931No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC13180.11081692589811737No Hit
CCATAGACGAGGAGTTTGGGAGCCTGGCCAGGTGTCTGCTGGTACCAGGCTAAGTAGCTGCTGCCAACACTCTGA13090.1100602094086765No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA12980.1091353336993599No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT12910.1085467764297948No Hit
GTGTTGGCAGCAGCTACTTAGCCTGGTACCAGCAGACACCTGGCCAGGCTCCCAAACTCCTCGTCTATGGTGCAT12740.10711742306085094No Hit
CTCCCAAACTCCTCGTCTATGGTGCATCCAGGAGGGCCACTGGCGTCCCA12740.10711742306085094No Hit
GTCCTGGACCTCCTGTGCTAGACCATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGGGTC12650.10636070657141007No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA12580.10577214930184496No Hit
CTCCTGGATGCACCATAGACGAGGAGTTTGGGAGCCTGGCCAGGTGTCTGCTGGTACCAGGCTAAGTAGCTGCTG12480.10493135320246623No Hit
GCCTGGTACCAGCAGACACCTGGCCAGGCTCCCAAACTCCTCGTCTATGGTGCATCCAGGAGGGCCACTGGCGTC12460.10476319398259049No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC12430.10451095515277686No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC12270.10316568139377089No Hit
GTGTATTACTGTGCGAGAGTCGAGGAGGGGTGGGACCTCCCAGGCAACTA12090.10165224841488915No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA12050.10131592997513765No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)