FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686805

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686805
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1560041
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATATATAGCCGTGTCTGCGGCGGTCACAGAGTTCAGCCTCAGGGAGA68160.4369115939901579No Hit
ATATATTACTGTGCGAGACATCCCGGGTCATGGTATTACTCTATTTCGGGGAGTAACCCACTAAATTGGTTCGAC44920.28794115026464046No Hit
CTTCTACACTGGGGCCAGTTACTACAACCCGTCCCTCAAGAGTCGAGTCACCATTTCCGTTGACACGTCCAAGAG41500.2660186495098526No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG35770.229288845613673No Hit
GTCTCTGGTGGCTCCATCAGCAGTAGGACTCACTACTGGGGCTGGATCCG35380.22678891131707435No Hit
GACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCACAGTACGCAGG35160.22537869197027513No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT33510.21480204686928098No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC33250.21313542400488192No Hit
GTGTAGAAGATGCTCCCAATCCACTCCAGCCCCTTCCCTGGGGGCTGGCGGATCCAGCCCCAGTAGTGAGTCCTA32000.20512281407988633No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC30410.194930774255292No Hit
GTATTACTCTATTTCGGGGAGTAACCCACTAAATTGGTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTC28630.18352081772209833No Hit
GAGTAATACCATGACCCGGGATGTCTCGCACAGTAATATATAGCCGTGTC27770.17800814209370136No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG27520.17640562010870225No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTCCGCCGAAGACCACAGTACGCAGGCTGCTATCCCATGTT27250.17467489636490324No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA26660.17089294448030531No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCTTGGTCCCTC26250.1682648084249068No Hit
CACCTGCACTGTCTCTGGTGGCTCCATCAGCAGTAGGACTCACTACTGGG26220.16807250578670688No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG25750.16505976445490855No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC25360.16255983015830994No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT25110.16095730817331083No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG24860.15935478618831173No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC24470.1568548518917131No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG23170.14852173756971773No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT23170.14852173756971773No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT21940.14063732940352208No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG21890.14031682500652226No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC21800.1397399170919226No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC21800.1397399170919226No Hit
GTCCTGGGCCCAGTCTGTGTTGACGCAGCCGCCCTCAGTGTCTGCGGCCCCAGGACAGAAAGTCACCATCTC21480.1376886889511237No Hit
CCCAGGAACAGCCCCCAAACTCCTCATTTATGACAGTAATAAGCGACCCTCAGGGATTCCTGACCGATTCTCTGG21350.13685537751892418No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC20930.13416314058412568No Hit
CAGTAATATATAGCCGTGTCTGCGGCGGTCACAGAGTTCAGCCTCAGGGA20800.13332982915192612No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG20720.1328170221167264No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA20480.13127860101112726No Hit
CTACTGGGGCTGGATCCGCCAGCCCCCAGGGAAGGGGCTGGAGTGGATTG20390.13070169309652757No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC20150.12916327199092845No Hit
GGATAGCAGCCTGCGTACTGTGGTCTTCGGCGGAGGGACCAAGCTGACCGTCCTAAGTCAGCCCAAGGCTGCCCC19700.12627873241793003No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG19670.12608642977973017No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19530.12518901746813063No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG19420.12448390779473102No Hit
GTAATAAGCGACCCTCAGGGATTCCTGACCGATTCTCTGGCTCCGAGTCTGGCACGTCAGCCACCCTGGGCATCA19340.12397110075953131No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG19010.1218557717393325No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT18770.12031735063373335No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG18680.11974044271913366No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT18590.11916353480453398No Hit
GTTCCTGGGAGCTGCTGGTACCAGGATACAAAATTATTCCCAATGTTGGA18590.11916353480453398No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18420.11807381985473459No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT18410.11800971897533462No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC18320.11743281106073493No Hit
GTAATAATCGGCCTCGTCCCCAGTCTGGAGTCCGGTGATGCCCAGGGTGG18290.11724050842253506No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG18020.11550978467873602No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17990.11531748204053612No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA17910.1148046750053364No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17620.11294574950273743No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC17560.11256114422633764No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT17120.10974070553273921No Hit
ACCAGAGACAGTGCAGGTGAGGGACAGGGTCTCCGAAGGCTTCACCAGTCCTGGGCCCGACTCCTGCAGCTGCAA17020.10909969673873955No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC17010.1090355958593396No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG16940.10858688970353984No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC16940.10858688970353984No Hit
ATATTACTGTGCGAGACATCCCGGGTCATGGTATTACTCTATTTCGGGGAGTAACCCACTAAATTGGTTCGACCC16810.10775357827134031No Hit
GATTATTACTGCGGAACATGGGATAGCAGCCTGCGTACTGTGGTCTTCGG16530.1059587536481413No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG16480.10563824925114147No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG16320.10461263518074206No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG16270.10429213078374222No Hit
CTGCTATCCCATGTTCCGCAGTAATAATCGGCCTCGTCCCCAGTCTGGAG16200.10384342462794248No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC16070.10301011319574294No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT15770.10108708681374401No Hit
GTTCTGGAAGTGACTCCAACATTGGGAATAATTTTGTATCCTGGTACCAGCAGCTCCCAGGAACAGCCCCCAAA15650.10031787626094443No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15630.10018967450214449No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC2300.047.99446541
GGTATCA10100.047.327291
AACGGAA21450.046.95042870
TAAGGCG2700.044.05603829
TCGTATG2450.043.5627740
TCTCGTA2300.043.280438
GGCGAAT2750.042.12610632
AGACTAA2900.042.1057325
AATCTCG2450.041.894336
GCGAATC2650.039.89062533
TTATACA3250.039.638222
TATACAC3350.039.466963
GACTAAG3100.039.3993426
AGGCGAA3000.038.5845231
ATGCCGT2700.038.52999544
GTATCAA25900.038.3514481
CTTATAC3500.036.806921
CGGCTCG750.001894109836.78855570
AGGGGGG3400.036.5180570
CACGAGA3350.035.42133721
Produced by FastQC (version 0.11.5)