FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686807

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686807
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1779526
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC89330.5019876079360459No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACATGATCACTACTAAGATCCCACAC76980.43258710465595895No Hit
CTACTAAGATCCCACACCTGACAGAAATAGTCGGCCTCATCCCCAGCTTC66490.37363882292250855No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT66460.3734702387040144No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACATGATCACTA64390.3618379276279189No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG61430.3452042847364973No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG55850.3138476200965875No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC52250.2936175138772909No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC51000.28659317144003515No Hit
ATATATTACTGTGCGAGAGCCGTCGACTACGGTGACCCTACGCATCCTTT49130.2760847551539005No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT46340.26040642283394566No Hit
GTAATATATAGCCGTGTCCTCGGCTCTCAGGCTGGTCATTTCCAGAAACA46220.2597320859599691No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC46160.25939491752298083No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG40920.2299488740260047No Hit
GCCCTCAGGGGTCCCTGAGCGATTCTCTGGCTACAACTCTGGGAACACGG40200.22590285278214534No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT39340.2210701051853134No Hit
GGGTCTGTGAATTCCTATGCCGTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAATGACGGCCAGGATCAC39160.22005859987434856No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGGTCTGTGAATTCCTATGCCGTGACT38200.21466390488253614No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC38130.2142705417060498No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG37150.20876345723524128No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG36760.2065718623948175No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG36650.205953720260339No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA36440.20477363073088No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG36420.20466124125188392No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC36380.20443646229389173No Hit
TCTCTGATGATGGCGACCGGCCCTCAGGGGTCCCTGAGCGATTCTCTGGCTACAACTCTGGGAACACGGCCACC36340.20421168333589956No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT35840.20140194636099726No Hit
TCTTAGTAGTGATCATGTGGTTTTCGGCGGAGGGACCAAGCTGACCGTCC35390.19887318308358515No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT35360.19870459886509104No Hit
GCACAGGGTCTGTGAATTCCTATGCCGTGACTCAGCCACCCTCGGTGTCA34960.1964568092851692No Hit
CGGCCACCCTGACCATCAGCAGGGTCGAAGCTGGGGATGAGGCCGACTAT34500.19387185126825907No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA33830.19010680372188998No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGGTCTG33090.18594839299903457No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC32990.1853864456040541No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG32940.18510547190656387No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA32700.1837567981586108No Hit
CCTCTCTCACTGCACAGGGTCTGTGAATTCCTATGCCGTGACTCAGCCAC32520.18274529284764593No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTC32080.18027272430973193No Hit
CATCAGAGACGACCAGGAGAGGGGCCTGGCCTGGCTTTTGTTGGTACCAG31240.17555236619189604No Hit
CTCACTGCACAGGGTCTGTGAATTCCTATGCCGTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAATGACGG31070.17459705562042926No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA30430.17100059229255432No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACATGATCACTAC30220.16982050276309535No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGGTCTGTGAATTCCTATGCCGTGACTCAGC29500.16577448151923602No Hit
CTCCTGGTCGTCTCTGATGATGGCGACCGGCCCTCAGGGGTCCCTGAGCG29140.16375147089730638No Hit
CCTGAGGGCCGGTCGCCATCATCAGAGACGACCAGGAGAGGGGCCTGGCCTGGCTTTTGTTGGTACCAGTGAACA29010.16302093928383174No Hit
GGGTGGCCGTGTTCCCAGAGTTGTAGCCAGAGAATCGCTCAGGGACCCCTGAGGGCCGGTCGCCATCATCAGAGA29010.16302093928383174No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA27260.15318685987167371No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC27200.1528496914346854No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGGTCTGTGAATTCCTA26780.15048951237576746No Hit
GGAGAGGGGCCTGGCCTGGCTTTTGTTGGTACCAGTGAACACTTTCACTT26740.1502647334177753No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC26680.14992756498078702No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG26340.14801694383785347No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG26180.14711782800588472No Hit
CCTGTGGGGGAAACAACATTGGAAGTGAAAGTGTTCACTGGTACCAACAA25980.1459939332159238No Hit
GACCAGGAGAGGGGCCTGGCCTGGCTTTTGTTGGTACCAGTGAACACTTT25770.14481384368646483No Hit
TTCCTATGCCGTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAATGACGGCCAGGATCACCTGTGGGGGAAA25600.14385853311499802No Hit
GGATCACCTGTGGGGGAAACAACATTGGAAGTGAAAGTGTTCACTGGTACCAACAAAAGCCAGGCCAGGCCCCT25340.14239746988804883No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC25310.14222888566955472No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT25240.14183552249306838No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA25120.14116118561909183No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC25010.1405430434846133No Hit
GGCTACAACTCTGGGAACACGGCCACCCTGACCATCAGCAGGGTCGAAGCTGGGGATGAGGCCGACTATTT25000.14048684874511527No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT24900.1399249013501348No Hit
CCCTCGGTGTCAGTGGCCCCAGGAATGACGGCCAGGATCACCTGTGGGGGAAACAACATTGGAAGTGAAAGTGTT24480.13756472229121688No Hit
GTGAATTCCTATGCCGTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGG24100.13542932219029113No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC23910.13436162213982825No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT23910.13436162213982825No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC23820.13385586948434583No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA23400.1314956904254279No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG22950.12896692714801583No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC22790.12806781131604708No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC22660.12733727970257247No Hit
CTCCTGTACAGGGTCTGGATTCACCTTTAGTGGTTATTACATGAACTGGGTCCGCCAGTCTCCTGGGAAGGGG22630.12716869548407833No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT22570.12683152704709008No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG22560.12677533230759203No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT22400.12587621647562328No Hit
GTGTCAGTGGCCCCAGGAATGACGGCCAGGATCACCTGTGGGGGAAACAA22330.12548285329913694No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC22130.12435895850917604No Hit
GCTTGGTCCCTCCGCCGAAAACCACATGATCACTACTAAGATCCCACACCTGACAGAAATAGTCGGCCTCATCCC22070.12402179007218776No Hit
CCCCTCTCCTGGTCGTCTCTGATGATGGCGACCGGCCCTCAGGGGTCCCT21940.12329125845871317No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACATGATCA21910.12312267424021903No Hit
CTGCACAGGGTCTGTGAATTCCTATGCCGTGACTCAGCCACCCTCGGTGT21810.12256072684523857No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC21450.1205377162233089No Hit
ATCTTAGTAGTGATCATGTGGTTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCT21350.11997576882832844No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG21310.11975098987033626No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA20850.11716603185342614No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGGTCTGTGAATTC20820.116997447634932No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC20690.11626691602145742No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG20630.11592974758446913No Hit
GGCCAGGCCCCTCTCCTGGTCGTCTCTGATGATGGCGACCGGCCCTCAGG20430.1148058527945082No Hit
CCCCAGGAATGACGGCCAGGATCACCTGTGGGGGAAACAACATTGGAAGT20160.11328859482806096No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC20030.11255806321458636No Hit
CCCCTGAGGGCCGGTCGCCATCATCAGAGACGACCAGGAGAGGGGCCTGGCCTGGCTTTTGTTGGTACCAGTGAA19790.11120938946663325No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC19730.11087222102964496No Hit
GGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG19650.11042266311366061No Hit
GTTGTAGCCAGAGAATCGCTCAGGGACCCCTGAGGGCCGGTCGCCATCAT19570.10997310519767624No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT19380.10890540514721335No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG19240.10811867879424071No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC19230.10806248405474267No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC19160.10766912087825634No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT18890.1061518629118091No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC18890.1061518629118091No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG18690.10502796812184817No Hit
CCCTGACCATCAGCAGGGTCGAAGCTGGGGATGAGGCCGACTATTTCTGT18650.10480318916385599No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT18560.10429743650837357No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG18420.10351071015540093No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA18170.10210584166794978No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG18020.10126292057547909No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC17880.10047619422250645No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG17830.10019522052501621No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)