FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686808

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686808
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1779526
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC83990.47197961704408925No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACATGATCACTACTAAGATCCCACAC72550.40769283505832454No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG67200.37762864942686986No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT63070.35442022201417683No Hit
CTACTAAGATCCCACACCTGACAGAAATAGTCGGCCTCATCCCCAGCTTC60550.3402591476606692No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACATGATCACTA56680.31851178347492537No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC50780.28535688717107816No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG49330.27720864994386146No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG48540.2727692655235158No Hit
GGGTCTGTGAATTCCTATGCCGTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAATGACGGCCAGGATCAC47430.2665316494392327No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC47200.2652391704307776No Hit
ATATATTACTGTGCGAGAGCCGTCGACTACGGTGACCCTACGCATCCTTT47050.2643962493383069No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC42370.23809711125322136No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT42210.23719799542125264No Hit
GTAATATATAGCCGTGTCCTCGGCTCTCAGGCTGGTCATTTCCAGAAACA41980.23590551641279756No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT41480.23309577943789525No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG40980.23028604246299297No Hit
GCCCTCAGGGGTCCCTGAGCGATTCTCTGGCTACAACTCTGGGAACACGG40300.22646480017712584No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGGTCTGTGAATTCCTATGCCGTGACT38900.21859753664739937No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC38580.2167993049834619No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG37660.21162938894964167No Hit
GGGTGGCCGTGTTCCCAGAGTTGTAGCCAGAGAATCGCTCAGGGACCCCTGAGGGCCGGTCGCCATCATCAGAGA35610.20010946735254218No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA35360.19870459886509104No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGGTCTG34830.1957262776716946No Hit
TCTCTGATGATGGCGACCGGCCCTCAGGGGTCCCTGAGCGATTCTCTGGCTACAACTCTGGGAACACGGCCACC34610.19448999340273757No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC34330.1929165406967923No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG33970.19089353007486265No Hit
TCTTAGTAGTGATCATGTGGTTTTCGGCGGAGGGACCAAGCTGACCGTCC33070.18583600352003848No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG33030.1856112245620463No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTC32930.18504927716706585No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT32790.1842625508140932No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT32370.18190237175517526No Hit
CCTCTCTCACTGCACAGGGTCTGTGAATTCCTATGCCGTGACTCAGCCAC32300.18150900857868893No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA32210.1810032559232065No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32010.1798793611332456No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG31920.17937360847776318No Hit
CGGCCACCCTGACCATCAGCAGGGTCGAAGCTGGGGATGAGGCCGACTAT31540.17723820837683743No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGGTCTGTGAATTCCTATGCCGTGACTCAGC31080.1746532503599273No Hit
GCACAGGGTCTGTGAATTCCTATGCCGTGACTCAGCCACCCTCGGTGTCA31070.17459705562042926No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA30550.17167492916653088No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC30030.16875280271263246No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA29110.1635828866788122No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT28550.16043598126692166No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGGTCTGTGAATTCCTA28290.15897491803997243No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC28170.15830058116599588No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC28130.1580758022080037No Hit
CATCAGAGACGACCAGGAGAGGGGCCTGGCCTGGCTTTTGTTGGTACCAG28120.15801960746850566No Hit
CTCACTGCACAGGGTCTGTGAATTCCTATGCCGTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAATGACGG28070.1577386337710154No Hit
CTCCTGGTCGTCTCTGATGATGGCGACCGGCCCTCAGGGGTCCCTGAGCG27950.15706429689703885No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT27040.1519505756027167No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA26830.15077048607325771No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT26790.15054570711526555No Hit
CCTGTGGGGGAAACAACATTGGAAGTGAAAGTGTTCACTGGTACCAACAA26650.14975898076229288No Hit
GGAGAGGGGCCTGGCCTGGCTTTTGTTGGTACCAGTGAACACTTTCACTT26480.1488036701908261No Hit
CCTGAGGGCCGGTCGCCATCATCAGAGACGACCAGGAGAGGGGCCTGGCCTGGCTTTTGTTGGTACCAGTGAACA26050.1463872963924101No Hit
GACCAGGAGAGGGGCCTGGCCTGGCTTTTGTTGGTACCAGTGAACACTTT25860.14531959634194724No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACATGATCACTAC25810.145038622644457No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGGTCTGTGAATTC25720.14453286998897458No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG25630.1440271173334922No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC25380.14262224884604102No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC25260.14194791197206447No Hit
GGATCACCTGTGGGGGAAACAACATTGGAAGTGAAAGTGTTCACTGGTACCAACAAAAGCCAGGCCAGGCCCCT24760.13913817499716216No Hit
TTCCTATGCCGTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAATGACGGCCAGGATCACCTGTGGGGGAAA24450.13739613807272275No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC24450.13739613807272275No Hit
CCCTCGGTGTCAGTGGCCCCAGGAATGACGGCCAGGATCACCTGTGGGGGAAACAACATTGGAAGTGAAAGTGTT23940.13453020635832239No Hit
GGCTACAACTCTGGGAACACGGCCACCCTGACCATCAGCAGGGTCGAAGCTGGGGATGAGGCCGACTATTT23900.1343054274003302No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC23800.13374348000534975No Hit
GTGAATTCCTATGCCGTGACTCAGCCACCCTCGGTGTCAGTGGCCCCAGG23440.13172046938342008No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG23190.13031560089596894No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC23190.13031560089596894No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG23130.12997843245898064No Hit
CTCCTGTACAGGGTCTGGATTCACCTTTAGTGGTTATTACATGAACTGGGTCCGCCAGTCTCCTGGGAAGGGG23020.12936029032450214No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC22470.1262695796521096No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT21930.12323506371921511No Hit
GTGTCAGTGGCCCCAGGAATGACGGCCAGGATCACCTGTGGGGGAAACAA21850.12278550580323074No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA21530.12098727413929328No Hit
GGCCAGGCCCCTCTCCTGGTCGTCTCTGATGATGGCGACCGGCCCTCAGG21460.12059391096280694No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC21440.12048152148381086No Hit
GCTTGGTCCCTCCGCCGAAAACCACATGATCACTACTAAGATCCCACACCTGACAGAAATAGTCGGCCTCATCCC21300.11969479513083822No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG20970.1178403687274027No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT20930.11761558976941051No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA20870.11727842133242222No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC20850.11716603185342614No Hit
CCCCTCTCCTGGTCGTCTCTGATGATGGCGACCGGCCCTCAGGGGTCCCT20690.11626691602145742No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAACCACATGATCA20570.11559257914748083No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA20540.11542399492898671No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC20350.11435629487852383No Hit
ATCTTAGTAGTGATCATGTGGTTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCT20150.11323240008856292No Hit
CCCCTGAGGGCCGGTCGCCATCATCAGAGACGACCAGGAGAGGGGCCTGGCCTGGCTTTTGTTGGTACCAGTGAA20000.11238947899609222No Hit
CTGCACAGGGTCTGTGAATTCCTATGCCGTGACTCAGCCACCCTCGGTGT19780.11115319472713521No Hit
GTTGTAGCCAGAGAATCGCTCAGGGACCCCTGAGGGCCGGTCGCCATCAT19420.10913018410520556No Hit
GGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG19240.10811867879424071No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG19060.10710717348327589No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG19040.10699478400427978No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG18820.10575849973532278No Hit
CCCCAGGAATGACGGCCAGGATCACCTGTGGGGGAAACAACATTGGAAGT18540.10418504702937748No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT18460.1037354891133931No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC18430.10356690489489898No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC18140.10193725744945564No Hit
GTACAGGAGAGTCTCAGGGAGTTCCCAGGCTTGACCAGGCCTCCCCCAGA18090.1016562837519654No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC18030.10131911531497714No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAAC18000.101150531096483No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG17970.10098194687798885No Hit
CCCTGACCATCAGCAGGGTCGAAGCTGGGGATGAGGCCGACTATTTCTGT17920.10070097318049863No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC17810.10008283104602012No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGACG200.006431990353.5434559
GTTTATC200.00744316551.60256639
TAACCGG200.007788070551.014714
GTGCGAC200.00781842550.9645275
GTGTGCG200.00782103150.960233
GGTATCA10550.049.6167261
GTGGTAT2550.047.9868431
TGGTATC2800.043.685112
TCTGGAG15300.042.32195770
GTATCAA25800.037.1526031
ACAATGG17850.036.6618870
GTATCAG502.0318359E-433.990681
AACTGTA1451.2971486E-633.25518470
TATCAAC31150.030.6504782
TACCGCG450.004968107730.2190368
GGTATAA450.00496879130.21818518
GATTGAC1251.4915713E-1029.9117981
CTCTGGA14900.029.6144269
CCTCGTT950.006069432529.00452270
TTAACGG605.6472357E-428.55192235
Produced by FastQC (version 0.11.5)