FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686811

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686811
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1288005
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT45620.3541911716181226No Hit
GTCTCTGGTGTCTCCGTCAGTAGTGCTAGTTACTACTGGACATGGATCCG40630.31544908598957305No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC39990.3104801611794985No Hit
CACCTGCACTGTCTCTGGTGTCTCCGTCAGTAGTGCTAGTTACTACTGGA34410.2671573479916615No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG30750.238741309234048No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC29710.23066680641767695No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG29250.2270953917104359No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC28680.22266994305146331No Hit
GAGATACACGGCCGTGTCTGCGGCGGTCACAGACCTCAAGTTCAGGGAGAACTGGTTCTTGGACGTGTCCACTGA28600.22204882745020402No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC28480.22111715404831506No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT28200.21894324944390747No Hit
GTCCAGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGCGTC27820.21599295033792573No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA27570.21405196408399038No Hit
GGGTATTAGCAGCTGGTTAGCCTGGTATCAACAGAAACCAGGGAAGGCCCCTCAACTCCTGATCTATGCCTCATC26870.20861720257297137No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT26170.20318244106195238No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG25610.1988346318531372No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG25480.19782531900109082No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC25110.1949526593452665No Hit
AGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC25040.19440918319416461No Hit
GTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCTTGGCCGAATGTC23830.18501480972511752No Hit
GTAGGAGACAGAGTCACCATCACTTGTCGGGCGAGTCAGGGTATTAGCAGCTGGTTAGCCTGGTATCAACAGAAA22800.1770179463589039No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC22470.1744558445037092No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT22300.1731359738510332No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC21910.17010803529489404No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG21780.16909872244284765No Hit
GTCCATACCAGCGGGAGCATCAACTACAACCCCTCCCTCAAGAGTCGAGT21610.16777885179017163No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG20870.16203353247852298No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20760.16117949852679142No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC20610.16001490677443023No Hit
GTGTATCTCTGTGCGAGAGACTCGCTCAGTGGAGCGCCCCTTGATAAGTGGGGCCGGGGAACCCTAGCCACCGT19920.15465778471356867No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG19800.1537261113116797No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19630.15240624065900366No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC19300.14984413880380898No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA19130.14852426815113295No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG19060.14798079200003106No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18990.14743731584892916No Hit
CCTTGGGGCTGGTCGGGGATGCTGAAGAGACGGTGGCTAGGGTTCCCCGGCCCCACTTATCAAGGGGCGCTCCA18800.14596216629593828No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG18770.14572924794546604No Hit
CTCCTGATCTATGCCTCATCCACTTTACATGGTGGGGTCCCATCAAGGTT18750.14557396904515124No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC18630.14464229564326225No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTTCCAGATGCGACAT18210.14138143873665088No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG18070.14029448643444709No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG17820.13835350018051173No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT17670.1371889084281505No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC17390.1350150038237429No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17230.1337727726212243No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAAGAGACGGTGGCTAGGGTTCCCCGG17220.13369513317106688No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16870.1309777524155574No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16730.1298908001133536No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGACTTCCACCTTGGTCCCTTGGCCGAATGTCCGAGGGAAA16680.12950260286256654No Hit
GTCCCAGGTGCAGCTGCAGGAGTCGGGCCCAGGACTGGTGAAGCCTTCAGAGACCCTGTCCCTCACCTGCA16450.127716895508946No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT16320.12670758265689963No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC16310.1266299432067422No Hit
CACCATCAGCAGCCTGCAGTCTGACGATTTTGCAACTTACTTCTGTCAACAAACTAGCAGTTTCCCTCGGACATT15880.12329144684997341No Hit
GATTTCACTCTCACCATCAGCAGCCTGCAGTCTGACGATTTTGCAACTTACTTCTGTCAACAAACTAGCAGTTTC15720.12204921564745479No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC15220.11816724313958409No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15140.11754612753832477No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG15080.1170802908373803No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG14810.11498402568313011No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG14790.11482874678281528No Hit
ATGCAGGAGAAGGTGTCCCCCTTCTTCCAGTCCTCGGCTGCCACGCGCAG14690.11405235228124115No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA14680.11397471283108372No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT14540.11288776052887993No Hit
GGACTGGAGTGGATTGGCCGTGTCCATACCAGCGGGAGCATCAACTACAA14500.11257720272825028No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT14470.11234428437777803No Hit
GTCTGGGACAGATTTCACTCTCACCATCAGCAGCCTGCAGTCTGACGATTTTGCAACTTACTTCTGTCAACAAA14300.11102441372510201No Hit
GGAGTGGATTGGCCGTGTCCATACCAGCGGGAGCATCAACTACAACCCCT14220.1104032981238427No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC14200.11024801922352785No Hit
GGCTAGGGTTCCCCGGCCCCACTTATCAAGGGGCGCTCCACTGAGCGAGT14170.11001510087305562No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT14160.1099374614228982No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA14130.10970454307242597No Hit
GGTATGGACACGGCCAATCCACTCCAGTCCCTTCCCGGCGGACTGCCGGA14060.10916106692132406No Hit
ACTTTACATGGTGGGGTCCCATCAAGGTTCAGCGGCCGTGGGTCTGGGACAGATTTCACTCTCACCATCAGCAGC13890.10784119626864802No Hit
GTCCCTCACCTGCACTGTCTCTGGTGTCTCCGTCAGTAGTGCTAGTTACTACTGGACATGGATCCGGCAGTCCGC13700.10636604671565716No Hit
CAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATG13600.10558965221408301No Hit
GTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGACATGGG13280.10310518980904577No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG13210.10256171365794386No Hit
CTACAACCCCTCCCTCAAGAGTCGAGTCACCATGTCAGTGGACACGTCCA13090.1016300402560549No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12900.10015489070306403No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGAT200.00726884751.91197642
GTGGTAT2350.039.387221
GATTCAC1150.038.7529641
GGTACGT501.07472595E-437.8771268
TCGTATG852.926754E-936.58495340
ATCTCGT852.9958755E-936.49640737
TGTTAGA8800.034.88514770
TACGACC400.002678624434.2814674
AATGGTA5850.033.4958370
CGTATGC958.609277E-932.7569841
GTACGTA652.6384692E-432.50981569
GAATACG1700.032.2611549
ATCAACG26400.032.2038043
CAACGCA26700.031.8419635
TCAACGC26750.031.7824464
AACGCAG26950.031.5465836
ACGCAGA28300.030.038217
ATGGCAA3050.029.98151870
AGAGTAC28300.029.67484511
TACGTAC1850.029.64653612
Produced by FastQC (version 0.11.5)