FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686813

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686813
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1062534
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTATAGTGCATCGAAATTGCAAAATGGGGTCCCATCAAGGTTCAGTGGCAGTGGATCTGGGACAGAATTCA50030.4708555208586266No Hit
GTACAATAGTTTCCCGTCCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCAT49690.46765562325534993No Hit
CTATTGTACTGTAGGCAGTAATAAGTTACAAAATCTTCTGGCTGCAGGCTGTTGATTGTGAGAGTGAATTCTGTC44220.41617491769675136No Hit
CTATAGATCAGGCGCTCAGGGGCTTTGCCTGGTTTCTGCTGATACCAGCC41250.3882228709857755No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT37560.3534945705266843No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC33620.31641340418283087No Hit
GTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG26960.25373305701276383No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC26460.24902732524323926No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC24650.23199257623756042No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22170.2086521466607186No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG21680.20404052952658455No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA21480.20215823681877473No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC19970.1879469268748106No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC19310.18173536093903817No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19130.18004129750200934No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18890.17778254625263756No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18510.1742061901077989No Hit
GTAGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGGGCATAGGAAATGATTTAGGCTGGTATCAGCAGAAA18380.17298269984772252No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG17820.16771228026585502No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC17810.16761816563046453No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16670.15688909719594857No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC16450.15481857521735776No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG16340.15378331422806235No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT16220.15265393860337645No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTAGGAGACAGAGTCACCATCACTT15850.1491716970939283No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT15790.14860700928158535No Hit
GTCTATTACTGCGCGAGGCTGACTTTTGCTTTCATTCGGGGAGTTCAGACTACTCTCGGGCTCGACTCCTGGGGC15660.14738351902150895No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCT15640.147195289750728No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG15190.1429601311581559No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT15110.14220721407503195No Hit
GTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCCCCTGGCCAAAAGTG14840.13966611891948869No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14390.1354309603269166No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTGCCAGGTGTGACAT14370.13524273105613563No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12940.1217843381952954No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12310.11585511616569447No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12270.1154786576241325No Hit
CTTTTGGCCAGGGGACCAAGCTGGAGATCAAACGAACTGTGGCTGCACCA12110.11397282345788463No Hit
GTAATAGACAGCCGTGTCTTCAGACTTCAGGCTACTCAGTTCCATGTAGA11800.11105526976077941No Hit
GAATTCACTCTCACAATCAACAGCCTGCAGCCAGAAGATTTTGTAACTTATTACTGCCTACAGTACAATAGTTTC11600.1091729770529696No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11560.10879651851140763No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG11400.10729068434515979No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11400.10729068434515979No Hit
AGTCAGGGCATAGGAAATGATTTAGGCTGGTATCAGCAGAAACCAGGCAA11130.10474958918961652No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11120.10465547455422602No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG11070.10418490137727358No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCAGCTTGGTCC10980.10333786965875916No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10880.10239672330485425No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC10770.10136146231555884No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGGC400.001798952937.1777364
CTTATAC2200.035.133981
GTATCAA20500.034.9178851
CGTATGC1102.7284841E-1134.51948541
TATCAAC22850.031.6209162
TCAACGC23500.030.6032894
CAACGCA23650.030.5512875
ATCAACG23600.030.4736123
CTGTACG450.00525670729.8709649
AACGCAG24200.029.718076
ATAGCGG500.005502518329.59272863
GTGGTAT1950.029.29791
TATACAC2550.028.993773
CTCGACT6200.028.96911661
ACGAGAC2450.028.81770722
TCGTATG1202.730303E-928.70517540
GCTCGAC6250.028.59351360
CGCAGAG25500.028.3308228
ACTACTC6100.028.28949449
ACGCAGA25850.027.9472317
Produced by FastQC (version 0.11.5)