FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686815

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686815
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1505022
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT54740.363715613459471No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA48780.32411486343721224No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC46300.30763669899842No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC45370.30145738733387284No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC44530.2958760735723464No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG44030.2925538630000093No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC43170.2868396608155894No Hit
CTCCTGCACTGGGACCGCCAGTGATGTTGGGAATTATGACCTTGTCTCCTGGTTTCAACACCACCCAGGCAAAGC42720.283849671300486No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAGCCACTAGGGACATATGAGCAGCAATAATAATCAG41800.27773680384738564No Hit
GTAATAAACAGCCGTGTCCTCAACTCTCAGGTTGTTCATTTGCAGATACA36350.24152470860891068No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC35590.23647494853895826No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC35330.23474739904134292No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG34620.2300298600286242No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGGACCGCCAGTGATGTT34080.22644187261050006No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAGCCACTAGGGACATATGAGCAGCAATAATAATCAGC33380.22179077780922807No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATA32200.21395036085851238No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT31120.20677438602226414No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT30570.20311995439269326No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG30170.20046218593482357No Hit
TCCCTGAGGGCCGCCGGCTGACCTCATAAATAATGAGTTTGGGGGCTTTGCCTGGGTGGTGTTGAAACCAGGAGA29610.19674131009380594No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT28870.19182443844674696No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT28710.19076133106359908No Hit
CCTCAGGGATGTCTCATCGCTTCTCTGGTACCAAGTCTGGCAACACGGCCTCCCTGACAATCTCTGGGCTCCAGG28480.189233114200324No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC27870.18518001730207267No Hit
GTGCAGGAGATGGTGATCGACTGTCCAGGAGACCCAGACACGGAGGCAGG27320.1815255856725018No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC27280.18125980882671483No Hit
CATATGTCCCTAGTGGCTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCA26540.17634293717965585No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG26140.17368516872178613No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG25400.16876829707472715No Hit
GTCGATCACCATCTCCTGCACTGGGACCGCCAGTGATGTTGGGAATTATGACCTTGTCTCCTGGTTTCAACACCA24750.16444942333068885No Hit
GTTTATTACTGTGCGAGAGATCTGGGAGTCGTCAGCTACTATGACAGTCCCTCCCAGTACTGGGGCCAGGGCACC24660.16385142542766817No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG23060.1532203515961893No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG22080.14670881887440848No Hit
GATTATTATTGCTGCTCATATGTCCCTAGTGGCTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAG21580.14338660830207134No Hit
CTCCTGTACAACGTCTGGATTCACCTTCAGAAATTATGAGATGAATTGGGTCCGCCAGGCTCCAGGGAAGGGG21340.1417919472273495No Hit
CTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGGGACAACGCCAAGAAGTCACTGTATCTGCAAATGAA21320.14165905880445603No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC21230.14106106090143533No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT20350.13521397029412194No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC20200.1342173071224208No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC20200.1342173071224208No Hit
GGACATATGAGCAGCAATAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGGCCGTGTTGC20070.13335353237361314No Hit
GTACTGGGAGGGACTGTCATAGTAGCTGACGACTCCCAGATCTCTCGCACAGTAATAAACAGCCGTGTCCTCAA20060.1332870881621664No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA19290.1281708838807672No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC18800.12491511751987679No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTC18550.12325401223370822No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT17850.11860291743243621No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC17400.11561292791733278No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA17340.11521426264865231No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG17250.11461626474563162No Hit
CTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCCCG17060.11335382472814351No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA16820.11175916365342169No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA16760.11136049838474121No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC16750.11129405417329447No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC16640.11056316784738031No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC16590.1102309467901466No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC16350.10863628571542476No Hit
GTCCAGGAGACCCAGACACGGAGGCAGGCTGAGTCAGGGCAGACTGGGCC16280.10817117623529757No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT16130.1071745130635964No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG16090.10690873621780944No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCACTGGGACCG15990.106244294103342No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT15940.1059120730461083No Hit
ACCACACACTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGGGACAACGCCAAGAAGTCACTGTATCTG15870.1054469635659811No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA15850.1053140751430876No Hit
GGCCACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTGGAAGGCAGATGGCAGCCC15830.10518118672019412No Hit
CAAGAGGCAGCGCTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGC15710.10438385618283322No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15690.10425096775993972No Hit
CTCCAGGGACAACGCCAAGAAGTCACTGTATCTGCAAATGAACAACCTGAGAGTTGAGGACACGGCTGTTTATTA15630.10385230249125926No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG15550.10332074879968532No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGC15420.10245697405087766No Hit
ACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACT15370.10212475299364394No Hit
ATAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGG15360.1020583087821972No Hit
GCCTGGAGCCCAGAGATTGTCAGGGAGGCCGTGTTGCCAGACTTGGTACC15150.1006629803418156No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCGT150.001544827876.7424565
AGGGGGG4550.066.1266670
AACACGA450.00918699548.92297770
TCTCGTA5250.048.1032338
TCGTATG5650.048.0994440
GCCGTCT5800.047.87039646
CGTATGC5700.047.83913841
ATGCCGT5800.047.52890844
ATCTCGT5450.046.1879137
TATACAC6050.046.0356523
CTTATAC6000.045.3140641
CCCGTGT656.909628E-445.15967670
TTATACA6000.044.761452
CCGTCTT6250.044.60280647
TCTACAT5900.044.36636432
TACATCT5550.044.36319434
GTATGCC6300.043.99679642
ACGAGAC5900.043.8429322
GGTATCA10900.043.8032531
CTACATC5700.043.6796933
Produced by FastQC (version 0.11.5)