FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686816

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686816
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1505022
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60570.40245258873292217No Hit
GGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGAC50980.33873258995549566No Hit
CCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGTGTCTGATCAGT50180.3334170530397562No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA44170.2934840819602637No Hit
GTCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTC43380.288234989255971No Hit
CTCCTGCACTGGGACCGCCAGTGATGTTGGGAATTATGACCTTGTCTCCTGGTTTCAACACCACCCAGGCAAAGC42740.2839825597233795No Hit
GTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCC42410.2817899007456369No Hit
GCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC40440.26870039109062854No Hit
CACTAGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGG39820.2645808499809305No Hit
GACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAGCCACTAGGGACATATGAGCAGCAATAATAATCAG38660.2568733214531083No Hit
GTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTC38320.25461421826391906No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTG35290.23448162219555593No Hit
GTAATAAACAGCCGTGTCCTCAACTCTCAGGTTGTTCATTTGCAGATACA34550.22956475054849695No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGGACCGCCAGTGATGTT33800.22458143468999126No Hit
ACACTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGC31690.2105617060747285No Hit
GCCTTGGGCTGACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATA30400.20199040279809863No Hit
GAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGAT29790.19793730589984732No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAG29770.19780441747695382No Hit
ACCTAGGACGGTGACCTTGGTCCCAGTTCCGAAGACATAGCCACTAGGGACATATGAGCAGCAATAATAATCAGC29130.19355198794436226No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT28910.19209021529253392No Hit
GGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC27970.18584445941654007No Hit
CCTCAGGGATGTCTCATCGCTTCTCTGGTACCAAGTCTGGCAACACGGCCTCCCTGACAATCTCTGGGCTCCAGG27320.1815255856725018No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT27180.18059536671224738No Hit
TCCTAGGTCAGCCCAAGGCCAACCCCACTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGG26810.1781369308887179No Hit
GTGCAGGAGATGGTGATCGACTGTCCAGGAGACCCAGACACGGAGGCAGG26160.1738180571446796No Hit
CATATGTCCCTAGTGGCTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAGCCCAAGGCCAACCCCA26090.17335294766455242No Hit
TCCCTGAGGGCCGCCGGCTGACCTCATAAATAATGAGTTTGGGGGCTTTGCCTGGGTGGTGTTGAAACCAGGAGA25640.170362958149449No Hit
GTTTATTACTGTGCGAGAGATCTGGGAGTCGTCAGCTACTATGACAGTCCCTCCCAGTACTGGGGCCAGGGCACC25390.1687018528632804No Hit
GAGTGACAGTGGGGTTGGCCTTGGGCTGACCTAGGACGGTGACCTTGGTC25290.16803741074881298No Hit
GTCGATCACCATCTCCTGCACTGGGACCGCCAGTGATGTTGGGAATTATGACCTTGTCTCCTGGTTTCAACACCA24610.16351920437043446No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGG23590.15674189480286668No Hit
GTTTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAG22930.15235657684738163No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTG22680.15069547156121305No Hit
CTCCTGTACAACGTCTGGATTCACCTTCAGAAATTATGAGATGAATTGGGTCCGCCAGGCTCCAGGGAAGGGG22410.148901477852151No Hit
GGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACTCAGC21060.1399315093068407No Hit
GTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCCACACTAGTGT20780.1380710713863319No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCACTCCCGCCTTGA20630.13707440821463074No Hit
GGATATTTATTGGGGTTTCATGAGTGCAGGGAGAAGGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCC20580.13674218715739703No Hit
GACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCT20520.1363435218887166No Hit
CTGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTCCAAGCCAACAAGGCC20320.13501463765978172No Hit
GATTATTATTGCTGCTCATATGTCCCTAGTGGCTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAGGTCAG20270.134682416602548No Hit
CTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTC19710.1309615407615304No Hit
CTACGCAGACTCTGTGAAGGGCCGATTCACCATCTCCAGGGACAACGCCAAGAAGTCACTGTATCTGCAAATGAA19600.1302306544356162No Hit
GGACATATGAGCAGCAATAATAATCAGCCTCGTCCTCAGCCTGGAGCCCAGAGATTGTCAGGGAGGCCGTGTTGC19060.1266426670174921No Hit
GTACTGGGAGGGACTGTCATAGTAGCTGACGACTCCCAGATCTCTCGCACAGTAATAAACAGCCGTGTCCTCAA18880.12544667121145073No Hit
GGGCTGGATGACTTGGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGC18540.12318756802226147No Hit
GCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCAC17510.11634381424324694No Hit
CCACCAAACCCTCCAAACAGAGCAACAACAAGTACGCGGCCAGCAGCTAC17180.11415115526550443No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA16830.1118256078648684No Hit
ACCTGAGCCTGACGCCCGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC16440.10923428361844544No Hit
AGTGTGGCCTTGTTGGCTTGGAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACAGTGGGGTTGGCCTTGGGCTGA16320.10843695308108453No Hit
CCCGTGGGTGGGGTTAGAGTTGGGAACCTATGAACATTCTGTAGGGGCCA16150.1073074014864899No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC16100.10697518042925619No Hit
GACTTCCACTGCTCGGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT15960.10604496146900179No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT15960.10604496146900179No Hit
AGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTCCTGGACAGTCGATCACCATCTCCTGCACTGGGACCG15890.10557985198887457No Hit
GGGATGGGGAGAGAGACCCCTCCCCTGGGATCCTGCAGCTCCAGGCTCCCGTGGGTGGGGTTAGAGTTGGGAAC15830.10518118672019412No Hit
GTCCAGGAGACCCAGACACGGAGGCAGGCTGAGTCAGGGCAGACTGGGCC15780.10484896566296041No Hit
CTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAGTGTGGCC15760.10471607724006692No Hit
CTCTCGGGACGTCTCCACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGC15500.1029885277424516No Hit
CTAGTGTGTCTGATCAGTGACTTCTACCCGGGAGCTGTGACAGTGGCCTG15470.10278919510811138No Hit
CTCCAGGGACAACGCCAAGAAGTCACTGTATCTGCAAATGAACAACCTGAGAGTTGAGGACACGGCTGTTTATTA15380.10219119720509069No Hit
GGGATGTCTCATCGCTTCTCTGGTACCAAGTCTGGCAACACGGCCTCCCT15350.10199186457075046No Hit
CTCCACTCCCGCCTTGACGGGGCTGCCATCTGCCTTCCAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGAT15300.10165964351351675No Hit
ACCATGGCCTGGGCTCTGCTGCTCCTCACCCTCCTCACTCAGGACACAGGGTCCTGGGCCCAGTCTGCCCTGACT15160.10072942455326235No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3000.043.0551
GGTATCA11450.042.8091351
TGGTATC3450.037.4316642
GTATCAA29900.037.3283651
GTTAGGT755.718357E-835.3577320
CGCCCAA400.002501174634.76427543
GTAGTTT1102.1713521E-433.60476770
TATCAAC34550.032.2021522
ATCAACG35700.030.978293
CGAAGGA600.00443622630.92566969
TCAACGC36200.030.733354
CAACGCA36250.030.6006365
AACGCAG36900.030.0695926
GCGTTGT8750.029.32548962
CGCAGAG38200.028.9730558
CGTTGTC9050.028.94237763
TTGTCCC9150.028.92350865
GGCGTTG9150.028.34322761
AGAGTAC38600.028.34073811
AATGGTA5750.028.2864570
Produced by FastQC (version 0.11.5)