FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686819

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686819
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1232134
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT76860.623795788445088No Hit
CTACAAAGGCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACG68410.5552155853178307No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC67600.5486416250180581No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC53380.4332320997553838No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC48580.3942752979789536No Hit
GCCTTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTAATTTTCAGTGTAAAAT46210.37504037710184124No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG43950.3566982162654387No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT41940.3403850555215585No Hit
CCCCTGGAGAGCCGGCCTCCATATACTGCAGGTCAAGTCAGAGCCTCCTG41830.3394922954808487No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTAATTTTCAGTGTAAAATCTGTGCCTGCTCCACTGCCA40800.33113281509965636No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG40730.3305646950737501No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC40020.32480233481098647No Hit
CCATATACTGCAGGTCAAGTCAGAGCCTCCTGCATAGCAATGGAAAGAATTATTTGGATTGGTACCTGCAGAAGC39450.32017621460003537No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA37300.3027268138043427No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG36880.29931809364890505No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT36650.29745141356378446No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG36210.293880373400945No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT34690.2815440528384088No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG34480.27983969276069No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT34130.2769990926311586No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG33810.27440197251272996No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC33290.27018165232028335No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT33260.26993817230918066No Hit
TCCATATACTGCAGGTCAAGTCAGAGCCTCCTGCATAGCAATGGAAAGAATTATTTGGATTGGTACCTGCAGAAG32750.26579901212043494No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT31720.2574395317392426No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG31340.25435545159860856No Hit
GTATATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGGGAGAGTGGAGACT30440.24705105126552793No Hit
ATATACTGCAGGTCAAGTCAGAGCCTCCTGCATAGCAATGGAAAGAATTA29900.24266841106567955No Hit
CCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCA28070.22781613038841556No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG27590.22392045021077256No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATATACT27470.22294653016636176No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTACTCGGGCC27420.2225407301478573No Hit
GATTTTACACTGAAAATTAGCAGGGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAAAGG26880.21815808994800892No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGA26200.21263920969634797No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC26170.21239572968524528No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG25530.20720148944838793No Hit
GTTCAGTGGCAGTGGAGCAGGCACAGATTTTACACTGAAAATTAGCAGGG25210.20460436932995923No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCC25100.2037116092892494No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC24960.20257536923743683No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA24590.1995724491005037No Hit
GTTTATTACTGCATGCAAGCTCTACAAAGGCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAA24570.19941012909310188No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG24500.19884200906719562No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG24300.1972188089931777No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC23980.194621688874749No Hit
GTGTAAAATCTGTGCCTGCTCCACTGCCACTGAACCTGTCAGTGACTCCG23780.1929984888007311No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA23050.18707380853056566No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATATACTGCAGGTCAA23020.186830328519463No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCC23010.1867491685157621No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA22940.1861810484898558No Hit
CTCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAATATCC22870.18561292846394953No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT22530.18285348833811907No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTAATTTTCAGTGTAAAATCTGTGCCTGCTCCACTGCCA21260.17254616786810525No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACT20760.16848816768306044No Hit
GCAGTATATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAATATCCCCA20570.1669461276127434No Hit
TTATTACTGCATGCAAGCTCTACAAAGGCCGATCACCTTCGGCCAAGGGA20470.16613452757573446No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA20100.16313160743880128No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT19950.16191420738328785No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT19800.16069680732777442No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA19770.16045332731667172No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC19730.16012868730186813No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA19710.15996636729446634No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT19490.15818084721304662No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT19410.15753156718343947No Hit
CTCCTGCATAGCAATGGAAAGAATTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGAT19380.15728808717233678No Hit
GGCCGAAGGTGATCGGCCTTTGTAGAGCTTGCATGCAGTAATAAACCCCA19290.1565576471390287No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC19210.15590836710942152No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA19110.1550967670724126No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC19100.15501560706871167No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT18990.15412284702800183No Hit
GCCTCCACCCTGCTAATTTTCAGTGTAAAATCTGTGCCTGCTCCACTGCC18850.1529866069761893No Hit
TTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCC18570.1507141268725642No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC18560.1506329668688633No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT18500.1501460068466579No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA18390.14925324680594804No Hit
GAATTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTACTCGGGC18330.14876628678374268No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC18290.1484416467689391No Hit
AGTCAGAGCCTCCTGCATAGCAATGGAAAGAATTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAG18260.1481981667578364No Hit
GTCGTGTCCCTTGGCCGAAGGTGATCGGCCTTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCT18030.1463314866727158No Hit
CCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATATACTGCAG17810.14454596659129607No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG17680.14349088654318443No Hit
GCCCTGGCTTCTGCAGGTACCAATCCAAATAATTCTTTCCATTGCTATGC17630.14308508652467997No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC17480.1418676864691665No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTC17420.14138072644696115No Hit
CGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT17380.14105608643215753No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA17170.1393517263544387No Hit
GTCACTGACAGGTTCAGTGGCAGTGGAGCAGGCACAGATTTTACACTGAA17140.13910824634333604No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC16920.13732272626191633No Hit
GTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTACTCGGGCCCCCGGAGTCACTGA16890.1370792462508136No Hit
GCTCTACAAAGGCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT16880.13699808624711274No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT16830.13659228622860825No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA16660.13521256616569302No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC16650.13513140616199212No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG16530.13415748611758138No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC16210.1315603659991527No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT16170.1312357259843491No Hit
CTCCACAGCTCCTGATCTATTTGGGTTCTACTCGGGCCCCCGGAGTCACT16100.13066760595844285No Hit
GGTCAAGTCAGAGCCTCCTGCATAGCAATGGAAAGAATTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC16010.12993716592513477No Hit
GCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTACTCGGGCCCCCGGAGTCACTGACAGGTT15750.12782700582891146No Hit
GTCAGTGACTCCGGGGGCCCGAGTAGAACCCAAATAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA15640.12693424578820162No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT15520.12596032574379087No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC15480.12563568572898726No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC15470.1255545257252864No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA15330.12441828567347382No Hit
GGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATATACTGCAGGT15290.12409364565867025No Hit
GTCTCCACAGCTCCTGATCTATTTGGGTTCTACTCGGGCCCCCGGAGTCA15280.12401248565496933No Hit
GGGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAAAGGCCGATCACCTTCGGCCAAGGGA15280.12401248565496933No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG15060.12222696557354963No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC15040.12206464556614784No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT14950.12133420553283977No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC14890.1208472455106344No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC14870.1206849255032326No Hit
ATGCAGGAGGCTCTGACTTGACCTGCAGTATATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGGGAGAGTGGAGA14750.11971100545882184No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG14330.1163022853033842No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT14310.11613996529598243No Hit
GGCACAGATTTTACACTGAAAATTAGCAGGGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTA14200.11524720525527254No Hit
CTGCAGGTCAAGTCAGAGCCTCCTGCATAGCAATGGAAAGAATTATTTGG13910.11289356514794656No Hit
GCTGTGGAGACTGCCCTGGCTTCTGCAGGTACCAATCCAAATAATTCTTTCCATTGCTATGCAGGAGGCTCTGA13830.11224428511833939No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT13150.10672540486667847No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA13090.10623844484447308No Hit
ATTTGGGTTCTACTCGGGCCCCCGGAGTCACTGACAGGTTCAGTGGCAGT13050.1059138048296695No Hit
CTGCAGGTACCAATCCAAATAATTCTTTCCATTGCTATGCAGGAGGCTCTGACTTGACCTGCAGTATATGGAGGC13030.1057514848222677No Hit
CCCCCGGAGTCACTGACAGGTTCAGTGGCAGTGGAGCAGGCACAGATTTTACACTGAAAATTAGCAGGGTGGAGG13010.10558916481486591No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT12990.10542684480746413No Hit
GGCTTCTGCAGGTACCAATCCAAATAATTCTTTCCATTGCTATGCAGGAG12870.10445292476305337No Hit
GGTTTATTACTGCATGCAAGCTCTACAAAGGCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAA12860.10437176475935248No Hit
TCCTCAGCCTCCACCCTGCTAATTTTCAGTGTAAAATCTGTGCCTGCTCCACTGCCACTGAACCTGTCAGTGACT12730.10331668471124084No Hit
CCTCCATATACTGCAGGTCAAGTCAGAGCCTCCTGCATAGCAATGGAAAG12680.10291088469273635No Hit
GTGGAGACTGCCCTGGCTTCTGCAGGTACCAATCCAAATAATTCTTTCCA12620.10242392467053098No Hit
GGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT12600.10226160466312918No Hit
CTCCGGGGGCCCGAGTAGAACCCAAATAGATCAGGAGCTGTGGAGACTGC12510.1015311646298211No Hit
ATCTGTGCCTGCTCCACTGCCACTGAACCTGTCAGTGACTCCGGGGGCCCGAGTAGAACCCAAATAGATCAGGAG12450.10104420460761573No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT12420.10080072459651304No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT12380.10047608458170945No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)