FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686820

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686820
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1232134
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT74290.6029376674939577No Hit
CTACAAAGGCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACG62050.5035978229640606No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC62050.5035978229640606No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC53350.4329886197442811No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC51670.41935373912253054No Hit
GCCTTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTAATTTTCAGTGTAAAAT48960.39735937811958766No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT45190.36676205672434975No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC44110.357996776324653No Hit
CCCCTGGAGAGCCGGCCTCCATATACTGCAGGTCAAGTCAGAGCCTCCTG40990.3326748551699734No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTAATTTTCAGTGTAAAATCTGTGCCTGCTCCACTGCCA39970.324396534792482No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG39410.31985157458523183No Hit
GGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGG37450.30394421385985615No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA37020.3004543337007176No Hit
CCATATACTGCAGGTCAAGTCAGAGCCTCCTGCATAGCAATGGAAAGAATTATTTGGATTGGTACCTGCAGAAGC36160.29347457338244054No Hit
GATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGT35390.28722525309747154No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT35190.28560205302345365No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC34200.27756721265706485No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG33830.27456429252013176No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG33020.2679903322203592No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT32770.26596133212783674No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG32640.2649062520797251No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT32410.2630395719946045No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT31960.25938737182806415No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG31230.25346269155789874No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG30740.24948585137655482No Hit
TCCATATACTGCAGGTCAAGTCAGAGCCTCCTGCATAGCAATGGAAAGAATTATTTGGATTGGTACCTGCAGAAG30390.24664525124702347No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT29060.23585097075480427No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA28030.22749149037361197No Hit
GTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATATACT27100.21994361002942864No Hit
GTATATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGGGAGAGTGGAGACT27060.21961897001462502No Hit
ATATACTGCAGGTCAAGTCAGAGCCTCCTGCATAGCAATGGAAAGAATTA26850.21791460993690623No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCC26490.21499284980367397No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG26120.2119899296667408No Hit
CCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCA26030.21125948963343272No Hit
GTTCAGTGGCAGTGGAGCAGGCACAGATTTTACACTGAAAATTAGCAGGG25460.20663336942248167No Hit
GATTTTACACTGAAAATTAGCAGGGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAAAGG25220.20468552933366013No Hit
CCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTG25200.20452320932625837No Hit
GTGTAAAATCTGTGCCTGCTCCACTGCCACTGAACCTGTCAGTGACTCCG24990.20281884924853952No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA24560.199328969089401No Hit
AGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTACTCGGGCC24460.19851736905239203No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG24450.19843620904869114No Hit
GTTTATTACTGCATGCAAGCTCTACAAAGGCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAA24220.19656952896357052No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC23870.19372892883403914No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG23760.1928361687933293No Hit
CAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGA23740.1926738487859275No Hit
GCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCC23350.18950860864159255No Hit
GCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCC22820.18520712844544504No Hit
CTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATATACTGCAGGTCAA22570.18317812835292266No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC22060.17903896816417697No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA21910.1778215681086635No Hit
GGGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAAAGGCCGATCACCTTCGGCCAAGGGA21250.17246500786440436No Hit
GGCCGAAGGTGATCGGCCTTTGTAGAGCTTGCATGCAGTAATAAACCCCA21190.171978047842199No Hit
CTCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAATATCC21140.1715722478236945No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA21130.1714910878199936No Hit
GCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACT20840.16913744771266762No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT20600.1671896076238461No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACCCTGCTAATTTTCAGTGTAAAATCTGTGCCTGCTCCACTGCCA20540.16670264760164075No Hit
GTATCAACGCAGAGTACGGGGATCAGGACTCCTCAGTTCACCTTCTCACA20300.16475480751281923No Hit
GTCGTGTCCCTTGGCCGAAGGTGATCGGCCTTTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCT20230.16418668748691295No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT20090.16305044743510042No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC19710.15996636729446634No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19590.1589924472500556No Hit
CTCCTGCATAGCAATGGAAAGAATTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGAT19540.1585866472315511No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC19060.1546909670539081No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC19060.1546909670539081No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT19030.15444748704280542No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT18990.15412284702800183No Hit
TTATTACTGCATGCAAGCTCTACAAAGGCCGATCACCTTCGGCCAAGGGA18890.15331124699099286No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA18790.1524996469539839No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA18770.1523373269465821No Hit
GCCTCCACCCTGCTAATTTTCAGTGTAAAATCTGTGCCTGCTCCACTGCC18770.1523373269465821No Hit
GCAGTATATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGGGAGAGTGGAGACTGAGTCATCACAATATCCCCA18380.14917208680224717No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCT18290.1484416467689391No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC18060.14657496668381848No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC17990.1460068466579122No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC17960.14576336664680953No Hit
GCTCTACAAAGGCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCAT17620.14300392652097904No Hit
AGTCAGAGCCTCCTGCATAGCAATGGAAAGAATTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAG17610.14292276651727817No Hit
GTTCACCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAAT17440.14154304645436291No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA17120.13894592633593425No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC16800.13634880621750556No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTC16630.13496908615459033No Hit
TTGTAGAGCTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACCC16530.13415748611758138No Hit
GTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTACTCGGGCCCCCGGAGTCACTGA16390.13302124606576882No Hit
GAATTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTACTCGGGC16390.13302124606576882No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA16290.13220964602875987No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC16220.1316415260028536No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT16130.13091108596954554No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT16110.13074876596214374No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT16110.13074876596214374No Hit
GGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATATACTGCAGGT16070.13042412594734012No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC16020.13001832592883567No Hit
CCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGGCCTCCATATACTGCAG15860.1287197658696213No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA15700.12742120581040697No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC15600.12660960577339803No Hit
GTCACTGACAGGTTCAGTGGCAGTGGAGCAGGCACAGATTTTACACTGAA15400.1249864056993801No Hit
GGTCAAGTCAGAGCCTCCTGCATAGCAATGGAAAGAATTATTTGGATTGGTACCTGCAGAAGCCAGGGCAGTCTC15280.12401248565496933No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT15180.12320088561796039No Hit
CGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAAT15140.1228762456031568No Hit
GTCAGTGACTCCGGGGGCCCGAGTAGAACCCAAATAGATCAGGAGCTGTGGAGACTGCCCTGGCTTCTGCAGGTA15120.122713925595755No Hit
CTCCACAGCTCCTGATCTATTTGGGTTCTACTCGGGCCCCCGGAGTCACT15100.12255160558835322No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG15070.12230812557725051No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT15070.12230812557725051No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC14940.12125304552913889No Hit
GCCCTGGCTTCTGCAGGTACCAATCCAAATAATTCTTTCCATTGCTATGC14840.12044144549212991No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA14750.11971100545882184No Hit
GTGGAGACTGCCCTGGCTTCTGCAGGTACCAATCCAAATAATTCTTTCCA14710.11938636544401827No Hit
GCAGAAGCCAGGGCAGTCTCCACAGCTCCTGATCTATTTGGGTTCTACTCGGGCCCCCGGAGTCACTGACAGGTT14550.11808780538480393No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG14520.11784432537370125No Hit
GTCTCCACAGCTCCTGATCTATTTGGGTTCTACTCGGGCCCCCGGAGTCA14320.11622112529968331No Hit
GGGTCTCTGGATCCAGTGGGGATATTGTGATGACTCAGTCTCCACTCTCCCTGCCCGTCACCCCTGGAGAGCCGG14090.1143544452145627No Hit
GCTGTGGAGACTGCCCTGGCTTCTGCAGGTACCAATCCAAATAATTCTTTCCATTGCTATGCAGGAGGCTCTGA13930.11305588515534835No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC13860.1124877651294421No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT13820.11216312511463851No Hit
ACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGC13660.11086456505542416No Hit
GGCTTCTGCAGGTACCAATCCAAATAATTCTTTCCATTGCTATGCAGGAG13630.11062108504432148No Hit
GGCACAGATTTTACACTGAAAATTAGCAGGGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTA13600.11037760503321879No Hit
CTCCGGGGGCCCGAGTAGAACCCAAATAGATCAGGAGCTGTGGAGACTGC13560.1100529650184152No Hit
GTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACT13430.10899788497030355No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG13340.1082674449369955No Hit
GGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTG13300.1079428049221919No Hit
ATGCAGGAGGCTCTGACTTGACCTGCAGTATATGGAGGCCGGCTCTCCAGGGGTGACGGGCAGGGAGAGTGGAGA12850.10429060475565158No Hit
GGTTTATTACTGCATGCAAGCTCTACAAAGGCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAACGAA12790.1038036447334462No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG12510.1015311646298211No Hit
ATCTGTGCCTGCTCCACTGCCACTGAACCTGTCAGTGACTCCGGGGGCCCGAGTAGAACCCAAATAGATCAGGAG12340.10015144456690588No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)