FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686821

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686821
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences856181
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT35500.41463195282305965No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC32170.3757383076709247No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23610.275759448060632No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC23150.2703867523339107No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22450.2622109110106391No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG22050.2575390016830553No Hit
GTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG21860.25531984475245306No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC20660.2413041167697017No Hit
ACTTACTACTGTCTACAGCATTATGAATATCCGTACACTTTTGGCCAGGGGACCAAGCTGGAAATCGAACGAA20300.23709939837487634No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT19430.22693799558738165No Hit
CTTTTGGCCAGGGGACCAAGCTGGAAATCGAACGAACTGTGGCTGCACCA18900.22074771572833315No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA18440.21537502000161182No Hit
GTAGGAGACAGAGTCACCTTCACTTGTCGGGCGAGTCAGGGCATTAGCAGGTATTTAGCCTGGTTTCAGCAGAAA17960.2097687288085113No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17400.20322805574989403No Hit
GTCTACAGCATTATGAATATCCGTACACTTTTGGCCAGGGGACCAAGCTGGAAATCGAACGAACTGTGGCTGCAC17310.20217687615118768No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC17150.20030811242015412No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT17040.1990233373550686No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT16480.19248266429645133No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG16350.1909642937649866No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT15900.18570839577145487No Hit
GTGCAGCCACAGTTCGTTCGATTTCCAGCTTGGTCCCCTGGCCAAAAGTG15850.1851244071055069No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15770.18419002523999015No Hit
GTAGTAAGTTGCAATATCTTCAGGCTGCAGGCTGCTGATTGTGAGAATGA15510.1811532841770607No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14590.170407892723618No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG14510.16947351085810125No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG14490.1692399153917221No Hit
GGCCAAAAGTGTACGGATATTCATAATGCTGTAGACAGTAGTAAGTTGCA14090.1645680060641383No Hit
GCTGTAGACAGTAGTAAGTTGCAATATCTTCAGGCTGCAGGCTGCTGATTGTGAGAATGAAGTCTGTCCCAGATC13900.162348849133536No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG13890.1622320514003464No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT13750.1605968831356921No Hit
ATCTGGGACAGACTTCATTCTCACAATCAGCAGCCTGCAGCCTGAAGATA13610.15896171487103777No Hit
GTATTTAGCCTGGTTTCAGCAGAAACCAGGGAAACCTCCTAGGCGCCTGA13450.15709295114000427No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG13180.15393941234388525No Hit
GTCCCAGATCCACTGCCGGTGAACCTTGATGGGACCCCACTTTCCAAACT12900.1506690758145766No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTGCCCGGT12730.14868351435035349No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTCGATTTCCAGCTTGGTCC12660.14786593021802633No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT12500.14599716648699282No Hit
AGTCAGGGCATTAGCAGGTATTTAGCCTGGTTTCAGCAGAAACCAGGGAA12370.1444787959555281No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTGCCCGGTCTGACAT12280.14342761635682175No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG12190.1423764367581154No Hit
ATGAATATCCGTACACTTTTGGCCAGGGGACCAAGCTGGAAATCGAACGA12020.1403908752938923No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11950.13957329116156514No Hit
CCTCCTAGGCGCCTGATCTTTGGTGCATCCAGTTTGGAAAGTGGGGTCCCATCAAGGTTCACCGGCAGTGGAT11910.13910610022880676No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC11760.13735413423096285No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA11510.134434190901223No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA11400.13314941583613746No Hit
GTCTCCATCTGCCACGTCTGCATCTGTAGGAGACAGAGTCACCTTCACTT11170.13046306797277676No Hit
GCATTATGAATATCCGTACACTTTTGGCCAGGGGACCAAGCTGGAAATCGAACGAACTGTGGCTGCACCAT11080.12941188837407044No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11020.12871110197493288No Hit
GCCACAGTTCGTTCGATTTCCAGCTTGGTCCCCTGGCCAAAAGTGTACGG10640.12427278811372829No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC10500.12263761984907397No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT10340.12076885611804046No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA9900.11562975585769832No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT9850.11504576719175033No Hit
GTCTGACATCCAGATGACCCAGTCTCCATCTGCCACGTCTGCATCTGTAG9770.11411138532623358No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG9610.11224262159520008No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9470.11060745333054577No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC9360.10932267826546023No Hit
GGGCATTAGCAGGTATTTAGCCTGGTTTCAGCAGAAACCAGGGAAACCTCCTAGGCGCCTGATCTTTGGTGCATC9250.1080379032003747No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9170.10710352133485794No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA9020.10535155533701401No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG9020.10535155533701401No Hit
CATCAAGGTTCACCGGCAGTGGATCTGGGACAGACTTCATTCTCACAATCAGCAGCCTGCAGCCTGAAGATATTG8940.10441717347149726No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT8890.1038331848055493No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA8880.1037163870723597No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT8770.10243161200727416No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8700.10161402787494701No Hit
CCTAGGAGGTTTCCCTGGTTTCTGCTGAAACCAGGCTAAATACCTGCTAA8690.1014972301417574No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC8610.10056284827624067No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC8580.10021245507667187No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTTA200.005907001454.7057664
ACCGTAC750.054.3513227
GTACTAG850.052.07534430
GACCGTA800.050.93945726
CGTATGC850.048.80806741
GGTATCA7000.046.0207861
ATCTCGT851.8189894E-1244.41541337
TCGTATG950.043.58732240
ACTAGAT950.043.1107332
TCTCCGA950.042.8337511
GTGGTAT1600.042.387561
TAGATCT1000.041.04446834
CGTACTA1000.040.83038329
CGAGACC1050.038.7724623
GATCTCG1009.094947E-1237.70628736
ACGAGAC1100.037.0100822
TATGCCG1151.8189894E-1236.1854243
TACTAGA1250.035.4569831
GTATCAA17100.034.703271
GCGATGT400.002823422233.91401317
Produced by FastQC (version 0.11.5)