FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686822

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686822
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences856181
Sequences flagged as poor quality0
Sequence length20-76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT35540.41509914375581797No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC25340.29596545590243184No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24730.28884079417786657No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC23520.2747082684619257No Hit
GTCCCACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG23080.26956916820158355No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT20390.2381505779735827No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18900.22074771572833315No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG18830.219930131596006No Hit
CTTTTGGCCAGGGGACCAAGCTGGAAATCGAACGAACTGTGGCTGCACCA18330.21409024493652626No Hit
ACTTACTACTGTCTACAGCATTATGAATATCCGTACACTTTTGGCCAGGGGACCAAGCTGGAAATCGAACGAA18290.21362305400376788No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT17820.20813356054385695No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC17380.2029944602835148No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA17240.2013592920188605No Hit
GTCTACAGCATTATGAATATCCGTACACTTTTGGCCAGGGGACCAAGCTGGAAATCGAACGAACTGTGGCTGCAC17200.20089210108610212No Hit
GTAGGAGACAGAGTCACCTTCACTTGTCGGGCGAGTCAGGGCATTAGCAGGTATTTAGCCTGGTTTCAGCAGAAA16920.19762176455679348No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT16890.1972713713572247No Hit
ACTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTC16290.19026350736584904No Hit
GTGCAGCCACAGTTCGTTCGATTTCCAGCTTGGTCCCCTGGCCAAAAGTG16070.18769395723567797No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG15790.18442362070636933No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC15310.1788173295132688No Hit
GTAGTAAGTTGCAATATCTTCAGGCTGCAGGCTGCTGATTGTGAGAATGA15220.17776614991456247No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT14920.17426221791887464No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT14790.1727438473874099No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT14570.17017429725723884No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG13640.15931210807060656No Hit
GGCCAAAAGTGTACGGATATTCATAATGCTGTAGACAGTAGTAAGTTGCA13630.159195310337417No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG13490.15756014207276267No Hit
GCTGTAGACAGTAGTAAGTTGCAATATCTTCAGGCTGCAGGCTGCTGATTGTGAGAATGAAGTCTGTCCCAGATC13380.15627536700767714No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG13160.15370581687750604No Hit
GGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTGCCCGGT12890.150552278081387No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12860.1502018848818182No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT12710.1484499188839743No Hit
GTCCCAGATCCACTGCCGGTGAACCTTGATGGGACCCCACTTTCCAAACT12500.14599716648699282No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA12290.14354441409001134No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT12190.1423764367581154No Hit
GTATTTAGCCTGGTTTCAGCAGAAACCAGGGAAACCTCCTAGGCGCCTGA12130.14167565035897783No Hit
CAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGTTCCCAGGTGCCCGGTCTGACAT12120.14155885262578824No Hit
ATCTGGGACAGACTTCATTCTCACAATCAGCAGCCTGCAGCCTGAAGATA12010.1402740775607027No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG11940.13945649342837554No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA11790.13770452743053163No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTCGATTTCCAGCTTGGTCC11640.13595256143268772No Hit
GTCTCCATCTGCCACGTCTGCATCTGTAGGAGACAGAGTCACCTTCACTT11530.13466778636760218No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG11450.13373340450208543No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG11350.1325654271701895No Hit
ATGAATATCCGTACACTTTTGGCCAGGGGACCAAGCTGGAAATCGAACGA11210.13093025890553517No Hit
AGTCAGGGCATTAGCAGGTATTTAGCCTGGTTTCAGCAGAAACCAGGGAA11050.12906149517450166No Hit
GCATTATGAATATCCGTACACTTTTGGCCAGGGGACCAAGCTGGAAATCGAACGAACTGTGGCTGCACCAT11050.12906149517450166No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10960.1280103155757953No Hit
CCTCCTAGGCGCCTGATCTTTGGTGCATCCAGTTTGGAAAGTGGGGTCCCATCAAGGTTCACCGGCAGTGGAT10780.1259079563783826No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA10630.1241559903805387No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA10070.11761531732192143No Hit
GGGCATTAGCAGGTATTTAGCCTGGTTTCAGCAGAAACCAGGGAAACCTCCTAGGCGCCTGATCTTTGGTGCATC10060.11749851958873182No Hit
GCCACAGTTCGTTCGATTTCCAGCTTGGTCCCCTGGCCAAAAGTGTACGG9950.11621374452364629No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT9860.11516256492493994No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA9840.11492896945856075No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA9760.113994587593044No Hit
GTCTGACATCCAGATGACCCAGTCTCCATCTGCCACGTCTGCATCTGTAG9500.11095784653011455No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC9260.10815470093356429No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8950.10453397120468687No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT8910.10406678027192848No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC8730.10196442107451578No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC8620.10067964600943025No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT8580.10021245507667187No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGCGA200.007621160751.2927134
TGGTATC2900.041.99792
GTGGTAT3050.041.053651
GGTATCA7650.040.2559281
GTATCAA18800.035.101581
ATGGCAA3550.033.4962270
ATCAACG21000.031.4114253
TATCAAC21100.031.2662072
CAACGCA21300.030.970825
GACTCGA450.004549288730.76620742
GAATACG2100.030.631059
TTGTCCG2609.094947E-1230.49014770
AACGCAG21800.030.2640136
ATACGTA2200.029.24898511
TCAACGC23350.028.9744664
AATGGTA6450.028.67807270
CGCAGAG22800.028.6532488
ACGCAGA23050.028.482677
AGAGTAC22900.028.2473511
GTGCTAG2400.028.201271
Produced by FastQC (version 0.11.5)