FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686823

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686823
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1452762
Sequences flagged as poor quality0
Sequence length20-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC49980.34403432909175763No Hit
CCTATGAACTGACTCAGCCACCCTCGGTGTCAGTGGCCCCTGGACAGACG45080.31030547329844804No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACGCCCACTGAACACTATTATAATCCCACAC37810.2602628648051092No Hit
CTCCTGTACAGCCTCTGGATTCACCTTTGGCAACTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGA37680.25936801761059275No Hit
GATCAGGACTGAGCAGAGAAAACTCACCATGGAGCCTGGGCTGAGCTGGC36260.24959353287049083No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACGCCCACTGAACACTA34800.23954371053207615No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG33570.23107707938395966No Hit
GGACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGAACAGCCTTATTT33540.23087057618522513No Hit
CTATTATAATCCCACACCTGACAATAGTAGTCGGCCTCATCGCCGGCCGC32910.22653400901179957No Hit
CTCCAGTCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATGGCATAGTTGC31560.2172413650687449No Hit
CCCCTGTCCTGGTCGTCTTTGATGATAACGACCGCCCCTCAGGGATCCCT28860.19865607718263556No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG28650.19721055479149374No Hit
CCCTGAGACTCTCCTGTACAGCCTCTGGATTCACCTTTGGCAACTATGCC28450.1958338667999301No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG28450.1958338667999301No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT27930.19225447802186454No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA26910.18523336926488992No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC26200.18034612689483892No Hit
GTACGGAGGCTGCGTAATATGGGTTAGTTCCACTCCCATCAAGAGTCGCGACACACTCCAGTCCCTTCCCTGGAG25820.17773041971086798No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG25740.1771797445142425No Hit
CCCCTGGACAGACGGCCAGACTTACCTGTGGGGGATTCAACATTGGAAAT24970.17187949574672245No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT24210.16664808137878054No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC24100.16589090298342055No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC23010.15838795342939863No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC22820.15708009983741314No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT22560.15529040544838038No Hit
GAAATAGACGGCCGTGTCGTCGACTCTCAGGTTGACCATGTGCAGAAATA22470.15467089585217675No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA21810.15012782548001669No Hit
GGACTGGAGTGTGTCGCGACTCTTGATGGGAGTGGAACTAACCCATATTA21770.14985248788170397No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC21650.14902647508676575No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA21030.1447587423129184No Hit
GCCTCCGTACAGGGCCGGTTCACCATCTCGCGAGACAATTCCAGAAACAT20930.14407039831713658No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG20790.14310671672304204No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC20770.14296904792388568No Hit
GGACTGAGCAGAGAAAACTCACCATGGAGCCTGGGCTGAGCTGGCTTTTT20720.14262487592599477No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC20690.1424183727272602No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG20400.1404221751394929No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA20400.1404221751394929No Hit
ATTATAATCCCACACCTGACAATAGTAGTCGGCCTCATCGCCGGCCGCGA19810.13636094556438014No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG19800.13629211116480194No Hit
GACCAGGACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGAACAGCCT19240.1324373847884237No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT19170.13195554399137643No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG18890.13002818080318732No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG18850.12975284320487457No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC18820.12954634000614001No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACGCCCACTGAACACTAT18750.12906449920909274No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG18640.12830732081373272No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC18590.12796314881584184No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGACTCTGTGA18490.12727480482005998No Hit
GTACAGGAGAGTCTCAGGGAGTCCCCAGGCTTCACCACGCCTCCCCCCGACTCCAACAACTGCACCTCACACTGG18410.12672412962343454No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC18320.12610462002723088No Hit
GTACAGGGCCGGTTCACCATCTCGCGAGACAATTCCAGAAACATCGTATT18230.12548511043102725No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGACTCTGTGACCTCCTATGAACTGACT18200.1252786072322927No Hit
GTCCTGGTCGTCTTTGATGATAACGACCGCCCCTCAGGGATCCCTGAGCG17990.12383308484115085No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG17970.1236954160419945No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGACCCCTGG17740.12211222485169629No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG17660.12156154965507082No Hit
GACTTACCTGTGGGGGATTCAACATTGGAAATAAGGCTGTTCACTGGTACCAGCAGAAGCCAGGCCAGGCCC17530.12066670246055444No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG17380.1196341864668817No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC17310.11915234566983442No Hit
CCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCTAACTCTGGGAATGCGG17010.11708731368248892No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT16790.11557295689176893No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGACTCTGTGACCTCCTATGAACTGACTCAGC16710.11502228169514347No Hit
TTATAATAGTGTTCAGTGGGCGTTCGGCGGAGGGACCAAGCTGACCGTCC16590.11419626890020527No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC16460.11330142170568888No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16290.11213123691285978No Hit
GCCCTGTACGGAGGCTGCGTAATATGGGTTAGTTCCACTCCCATCAAGAGTCGCGACACACTCCAGTCCCTTCC16010.11020387372467066No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGACTCTG15940.10972203292762338No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT15900.10944669532931064No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG15800.10875835133352882No Hit
GCCCTGGGATTCTCAGGTGTTTTCATTTGCTGATCAGGACTGAGCAGAGA15720.10820767613690335No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15680.10793233853859062No Hit
CTGCACAGACTCTGTGACCTCCTATGAACTGACTCAGCCACCCTCGGTGT15660.10779466973943426No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGACCCCTGG15580.1072439945428088No Hit
GTCCAGGGGCCACTGACACCGAGGGTGGCTGAGTCAGTTCATAGGAGGTC15570.10717516014323063No Hit
CTCTGTGACCTCCTATGAACTGACTCAGCCACCCTCGGTGTCAGTGGCCCCTGGACAGACGGCCAGACTTAC15330.10552313455335423No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG15320.10545430015377603No Hit
GCCTTATTTCCAATGTTGAATCCCCCACAGGTAAGTCTGGCCGTCTGTCCAGGGGCCACTGACACCGAGGGTGG15280.1051789625554633No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15180.10449061855968148No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA14990.10318276496769602No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA14820.10201258017486692No Hit
CCTCTCTCACTGCACAGACTCTGTGACCTCCTATGAACTGACTCAGCCAC14790.10180607697613236No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT14770.10166840817697599No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14750.10153073937781963No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14680.10104889858077236No Hit
GAGTGGAACTAACCCATATTACGCAGCCTCCGTACAGGGCCGGTTCACCATCTCGCGAGACAATTCCAGAAACAT14550.10015405138625597No Hit
GTCCGGATCTGGCGGACCGCGAGTCTGGTTCGACTCCTGGGGCCAGGGGTCCCTGGTCACCGTCTCCTCAGCATC14530.1000163825870996No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)