FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686824

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686824
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1452762
Sequences flagged as poor quality0
Sequence length20-76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC47180.32476069720986644No Hit
CCTATGAACTGACTCAGCCACCCTCGGTGTCAGTGGCCCCTGGACAGACG40570.27926115908868765No Hit
CTCCTGTACAGCCTCTGGATTCACCTTTGGCAACTATGCCATGAGCTGGGTCCGCCAGGCTCCAGGGAAGGGA39400.27120753433804023No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACGCCCACTGAACACTATTATAATCCCACAC32390.22295462023373408No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG31230.2149698298826649No Hit
GATCAGGACTGAGCAGAGAAAACTCACCATGGAGCCTGGGCTGAGCTGGC30920.2128359634957412No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG30880.21256062589742847No Hit
GGACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGAACAGCCTTATTT30680.21118393790586482No Hit
CTATTATAATCCCACACCTGACAATAGTAGTCGGCCTCATCGCCGGCCGC30210.20794872112569024No Hit
CCCCTGTCCTGGTCGTCTTTGATGATAACGACCGCCCCTCAGGGATCCCT30090.20712270833075203No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACGCCCACTGAACACTA29680.20430049794804656No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC29330.20189129396281014No Hit
CTCCAGTCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATGGCATAGTTGC29170.2007899435695592No Hit
CCCTGAGACTCTCCTGTACAGCCTCTGGATTCACCTTTGGCAACTATGCC27800.1913596308273482No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT27690.19060245243198817No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25510.17559655332394433No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC24990.17201716454587881No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG24140.1661662405817333No Hit
CCCCTGGACAGACGGCCAGACTTACCTGTGGGGGATTCAACATTGGAAAT24010.16527139338721689No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA23930.16472071819059142No Hit
GTACGGAGGCTGCGTAATATGGGTTAGTTCCACTCCCATCAAGAGTCGCGACACACTCCAGTCCCTTCCCTGGAG23790.16375703659649687No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT22640.15584108064500585No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC22310.15356954545892582No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG22250.15315653906145674No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT22210.152881201463144No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA21550.14833813109098393No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC21350.14696144309942027No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC21140.14551592070827846No Hit
GGACTGGAGTGTGTCGCGACTCTTGATGGGAGTGGAACTAACCCATATTA21000.14455223911418388No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC20710.14255604152641657No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG20660.14221186952852566No Hit
ATTATAATCCCACACCTGACAATAGTAGTCGGCCTCATCGCCGGCCGCGA20590.14173002873147839No Hit
GTCCTGGTCGTCTTTGATGATAACGACCGCCCCTCAGGGATCCCTGAGCG20350.140078003141602No Hit
GCCTCCGTACAGGGCCGGTTCACCATCTCGCGAGACAATTCCAGAAACAT20270.13952732794497652No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC20250.13938965914582016No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT20080.13821947435299106No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG19920.13711812395974013No Hit
GGACTGAGCAGAGAAAACTCACCATGGAGCCTGGGCTGAGCTGGCTTTTT19510.13429591357703466No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGACTCTGTGACCTCCTATGAACTGACT19500.13422707917745647No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG18990.13071652479896914No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA18970.13057885599981278No Hit
GACCAGGACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGAACAGCCT18860.12982167760445276No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC18500.12734363921963818No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG18480.12720597042048182No Hit
GAAATAGACGGCCGTGTCGTCGACTCTCAGGTTGACCATGTGCAGAAATA18400.12665529522385635No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG18330.12617345442680908No Hit
GACTTACCTGTGGGGGATTCAACATTGGAAATAAGGCTGTTCACTGGTACCAGCAGAAGCCAGGCCAGGCCC18260.1256916136297618No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA18080.12445259443735451No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG17810.12259406564874356No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGACCCCTGG17810.12259406564874356No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG17790.1224563968495872No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG17650.12149271525549263No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGACTCTGTGA17440.12004719286435081No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG17280.11894584247109988No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC17190.11832633287489622No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG17180.11825749847531805No Hit
GTACAGGGCCGGTTCACCATCTCGCGAGACAATTCCAGAAACATCGTATT17100.11770682327869258No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16900.11633013528712893No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGACTCTGTGACCTCCTATGAACTGACTCAGC16790.11557295689176893No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC16780.11550412249219073No Hit
GGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGACCTGTACACCACGAGCAGCCAGCTGAC16770.11543528809261257No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG16540.11385209690231435No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC16530.11378326250273618No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGACTCTG16490.11350792490442345No Hit
GCCCTGTACGGAGGCTGCGTAATATGGGTTAGTTCCACTCCCATCAAGAGTCGCGACACACTCCAGTCCCTTCC16300.11220007131243796No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC16260.11192473371412523No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT16100.1108233833208743No Hit
GGCCAGGCCCCTGTCCTGGTCGTCTTTGATGATAACGACCGCCCCTCAGG15990.1100662049255143No Hit
CTCTGTGACCTCCTATGAACTGACTCAGCCACCCTCGGTGTCAGTGGCCCCTGGACAGACGGCCAGACTTAC15940.10972203292762338No Hit
GGGTGGCCGCATTCCCAGAGTTAGAGCCAGAGAATCGCTCAGGGATCCCTGAGGGGCGGTCGTTATCATCAAAGA15880.10930902653015429No Hit
TTATAATAGTGTTCAGTGGGCGTTCGGCGGAGGGACCAAGCTGACCGTCC15830.10896485453226337No Hit
GCCCTGGGATTCTCAGGTGTTTTCATTTGCTGATCAGGACTGAGCAGAGA15790.10868951693395064No Hit
GCCTTATTTCCAATGTTGAATCCCCCACAGGTAAGTCTGGCCGTCTGTCCAGGGGCCACTGACACCGAGGGTGG15760.10848301373521609No Hit
CCCCTCAGGGATCCCTGAGCGATTCTCTGGCTCTAACTCTGGGAATGCGG15660.10779466973943426No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA15540.10696865694449606No Hit
CTGCACAGACTCTGTGACCTCCTATGAACTGACTCAGCCACCCTCGGTGT15240.10490362495715058No Hit
GAGTGGAACTAACCCATATTACGCAGCCTCCGTACAGGGCCGGTTCACCATCTCGCGAGACAATTCCAGAAACAT15190.10455945295925967No Hit
GTCCGGATCTGGCGGACCGCGAGTCTGGTTCGACTCCTGGGGCCAGGGGTCCCTGGTCACCGTCTCCTCAGCATC15190.10455945295925967No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC14940.10283859296980512No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14810.10194374577528872No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGACCCCTGG14660.10091122978161599No Hit
GTCCAGGGGCCACTGACACCGAGGGTGGCTGAGTCAGTTCATAGGAGGTC14650.10084239538203779No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACACG203.8089702E-4109.1534170
TATACGT150.002660691766.913164
TTACGGA200.0039709960.4808168
TCGATAT200.006767312552.85931449
CGATTCG200.008181225550.3825629
GTGGTAT2300.048.0194471
GGTATCA7500.043.2854081
TGGTATC2600.042.464122
GACAGGT1500.040.1617241
ACCTCGA750.001456292238.81010470
ATTGGCG350.00156328938.2571647
AATGGTA3750.036.869670
CTATCGG559.050342E-636.54462412
AATAGCG759.2450864E-734.72971362
ATTCGCC502.1371401E-433.69899431
ACTATCG502.2130829E-433.4992411
TATCGGT502.2130829E-433.4992413
CAGCGTT400.003005215433.486559
GGCATAA400.003013342633.46811
CAAACGA751.4147117E-632.8902449
Produced by FastQC (version 0.11.5)