FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686826

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686826
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1455811
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTGTGGTATTCGG98880.6792090456796933No Hit
GTAATAATCAGCCTCATCCTCAGACTGGAGGCCACTGATGGCCAGGGAGG98000.6731643049818966No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC81790.5618174337190748No Hit
GTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCC72520.4981415856866036No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACCATTCAGGCTGTCATCCCATG67920.4665440774935758No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG65130.44737950187215236No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG62060.42629159966506636No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT60020.41227879168381065No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACCATTCAGG54140.3718889333848968No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCT52970.3638521758662354No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG52830.3628905125734041No Hit
GTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC49490.33994797401585786No Hit
GTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCC47430.3257977855641976No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC47030.3230501761561082No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC46380.31858531086796293No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC44750.30738880252999873No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT42550.2922769507855072No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG42300.2905596949054513No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG41880.28767470502695747No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT41480.28492709561886814No Hit
ATACCACACCATTCAGGCTGTCATCCCATGCTGCACAGTAATAATCAGCC40730.2797753279787005No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCAC39390.2705708364616011No Hit
CCCCTGAGGGCCGCTGATTATTACCACAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTTTA36770.25257399483861576No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG36620.2515436413105822No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG36170.24845258072648166No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC36090.2479030588448638No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG34970.24020975250221352No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAG34910.23979761109100012No Hit
GTCTGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTGTGGTATTCGGCGGAGGGAC34500.23698131144770854No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGCCTCCAGT34350.23595095791967502No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA34250.23526405556765265No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC34170.2347145336860348No Hit
TCCTCAGACTGGAGGCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACC33660.23121133169072086No Hit
GGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCA32650.22427361793529518No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA32020.21994613311755443No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG31970.21960268194154325No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT31820.2185723284135097No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT31680.21761066512067845No Hit
GCATGGGATGACAGCCTGAATGGTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG31350.21534388735900473No Hit
GGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT29600.20332309619861372No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA29410.20201798172977126No Hit
GAGTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTC28460.19549240938555898No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT28340.19466812656313218No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC27930.19185182691984057No Hit
GATTATTACCACAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGG27280.18738696163169533No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACACCATTCAGGC27100.1861505373980551No Hit
CTCCTCATCTGTGGTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATT26960.18518887410522383No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA26550.18237257446193222No Hit
CTCTTGTTCTGGAAGCAGCTCCAATATCGGAAGTAATATTGTAAACTGGTACCAGCAGCTCCCAGGAACGGCCCC26220.18010579670025847No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC26190.1798997259946518No Hit
GTGGTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGG26160.17969365528904507No Hit
ACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC26110.1793502041130339No Hit
GCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCA26000.17859461152580933No Hit
GGCCATCAGTGGCCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGCAG25800.17722080682176464No Hit
AGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGT25040.17200034894639482No Hit
GATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTTTACAA25010.1717942782407881No Hit
GTCTGGCACCTCAGCCTCCCTGGCCATCAGTGGCCTCCAGTCTGAGGATG24810.17042047353674344No Hit
CCCAGGAACGGCCCCCAAACTCCTCATCTGTGGTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGG24740.16993964189032779No Hit
ATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCAGACTGGAGGCCAC24250.16657382036541832No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA24040.16513132542617137No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC24020.16499394495576694No Hit
GTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAG23500.16142205272525073No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT23240.15963610660999264No Hit
ATTGTAAACTGGTACCAGCAGCTCCCAGGAACGGCCCCCAAACTCCTCAT23230.1595674163747904No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC23110.1587431335523636No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC23090.15860575308195912No Hit
CTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTC23000.157987540965139No Hit
GTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCC22970.15778147025953232No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC22600.15523993155704965No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT22450.15420957802901614No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT22290.1531105342657804No Hit
GTTTGGGGGCCGTTCCTGGGAGCTGCTGGTACCAGTTTACAATATTACTT22100.15180541979693793No Hit
CCATTCAGGCTGTCATCCCATGCTGCACAGTAATAATCAGCCTCATCCTC21940.15070637603370218No Hit
GTGCAGCATGGGATGACAGCCTGAATGGTGTGGTATTCGGCGGAGGGACC21920.1505689955632977No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG21830.14995078344647758No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACAC21530.14789007639041057No Hit
GTACGGGAGCTTCAGCTGTGGGTAGAGAAGACAGGACTCAGGACAATCTCCAGCATGGCCAGCTTCCCTCTCCTC21440.14727186427359046No Hit
CTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCCAGAGAATCGGTC21120.14507377674711897No Hit
ATGCTGCACAGTAATAATCAGCCTCATCCTCAGACTGGAGGCCACTGATG20890.14349390133746756No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG20540.14108974310538938No Hit
GCCCCCAAACTCCTCATCTGTGGTAATAATCAGCGGCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGT20400.1401280798125581No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC20190.13868558487331115No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA20080.1379299922860866No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC19860.13641880711163745No Hit
GGATGACAGCCTGAATGGTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC19720.13545714381880616No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTC19540.13422071958516593No Hit
GTGCCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGGGCCGCTG19180.1317478711178855No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCAC19110.13126703947146987No Hit
GGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGC19090.13112965900106538No Hit
GTTTACAATATTACTTCCGATATTGGAGCTGCTTCCAGAACAAGAGATGGTGACCCTCTGCCCGGGGGTCCCAGA18990.13044275664904303No Hit
AGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGG18850.12948109335621175No Hit
GGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAATATCGGAAGTAATATT18670.12824466912257154No Hit
GCCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAG18500.12707693512413357No Hit
ATCTCCAGCATGGCCAGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTG18500.12707693512413357No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC18230.12522229877367325No Hit
GGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAG18160.1247414671272576No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC18150.12467277689205536No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAAC18020.12377980383442631No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC17850.12261206983598832No Hit
GCTTGGTCCCTCCGCCGAATACCACACCATTCAGGCTGTCATCCCATGCTGCACAGTAATAATCAGCCTCATCCT17810.1223373088951794No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG17690.12151302607275258No Hit
GGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATAC17320.11897148737026991No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA17310.11890279713506766No Hit
GTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAG17310.11890279713506766No Hit
GTCCCTGACCGATTCTCTGGCTCCAAGTCTGGCACCTCAGCCTCCCTGGC17250.11849065572385427No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC17120.11759768266622521No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT16930.11629256819738276No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG16850.11574304631576489No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG16680.11457531231732691No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA16510.11340757831888892No Hit
TCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAG16410.11272067596686658No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC16320.11210246385004648No Hit
GAACAAGAGATGGTGACCCTCTGCCCGGGGGTCCCAGACGCTGAGGGTGG16140.11086603961640626No Hit
GCACAGTAATAATCAGCCTCATCCTCAGACTGGAGGCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAG15880.10908009350114815No Hit
GTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAATATCGGAAGTAATATTGTAAA15850.10887402279554145No Hit
CCCTCAGCGTCTGGGACCCCCGGGCAGAGGGTCACCATCTCTTGTTCTGGAAGCAGCTCCAATATCGGAAGTAAT15790.10846188138432804No Hit
GTCAGGGACCCCTGAGGGCCGCTGATTATTACCACAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTA15730.10804973997311464No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG15670.10763759856190122No Hit
ATTCAGGCTGTCATCCCATGCTGCACAGTAATAATCAGCCTCATCCTCAG15590.10708807668028336No Hit
TTACTGTGCAGCATGGGATGACAGCCTGAATGGTGTGGTATTCGGCGGAG15390.10571427197623867No Hit
GACTGGAGGCCACTGATGGCCAGGGAGGCTGAGGTGCCAGACTTGGAGCC15310.10516475009462081No Hit
GGGTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG15270.10488998915381186No Hit
GTTCCTGGGAGCTGCTGGTACCAGTTTACAATATTACTTCCGATATTGGA15270.10488998915381186No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGCTGACTCAGCCACCCTCAGCGTCTGGGA15240.10468391844820515No Hit
TTATTACCACAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGCTGCTGGTA15230.10461522821300293No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAGGTTCTCAACCCTCACCCCCCAC15090.10365356492017164No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA14830.10186761880491356No Hit
GCCGAATACCACACCATTCAGGCTGTCATCCCATGCTGCACAGTAATAAT14820.1017989285697113No Hit
CCTCAGCCTCCCTGGCCATCAGTGGCCTCCAGTCTGAGGATGAGGCTGAT14760.1013867871584979No Hit
GGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGG14590.10021905316005993No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23150.040.62351
AGAGTCG350.001479130238.6904611
GCTTAGA350.001484636738.6612171
ATAATAG350.001486643138.6505933
GGTATCA9600.036.6476141
TATCAAC25500.036.4742622
TGTGTCG5900.035.78759870
ATCAACG26900.034.57363
TCAACGC27450.033.880874
CAACGCA28000.033.458075
CTCGAGC552.325174E-433.21833464
GCCAGAG46850.032.90014670
AATGGTA5350.032.88891270
AACGCAG28600.032.880056
ACGCAGA30100.031.2479537
CGCAGAG30250.031.0983458
AGAGTAC30250.030.44075811
GCAGAGT31100.030.0390369
CAGAGTA30950.029.86063210
GTCGGAA500.005295570529.82651967
Produced by FastQC (version 0.11.5)