FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686828

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686828
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1382744
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATAAGGTGACTCAGCCTCCCTCAGTGTCTGTGTCCTCAGGACAGACA48490.35067951840687794No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC48380.3498839987734534No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG47410.34286896200598227No Hit
GCCTTGGGCTGACCCAGGACGGTCAGTTTGGTCCCTCCGCCGAACACTACTGCAGTAGTGAAGTCCCACGTCTGA39310.2842897889992652No Hit
GGCTATGGATGAGGCTGACTATTACTGTCAGACGTGGGACTTCACTACTG32610.23583541132704247No Hit
TCCATAGCCTGTGTCCCGGTGATGGTCAGAGTGGCTGTGTTCCCATAATAGGAGCCGGAGAATCGCTCAGGGATC30510.22064821832530102No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG28620.2069797446237337No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT26860.19425143048894083No Hit
GTATTTAGATAAATGACAACCACAGGGGATTGGCCTGGCTTCTGTTGATA25550.18477751485452115No Hit
ATCTAAATACTGAGCGGCCCTCAGGGATCCCTGAGCGATTCTCCGGCTCCTATTATGGGAACACAGCCACTCTGA23440.16951800188610475No Hit
CTACTGCAGTAGTGAAGTCCCACGTCTGACAGTAATAGTCAGCCTCATCC23350.16886712218603012No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC23030.1665528832524314No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC22150.16018872618503496No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21860.15809144715146115No Hit
CTGCAGTAGTGTTCGGCGGAGGGACCAAACTGACCGTCCTGGGTCAGCCC20560.14868985148371644No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT20400.14753273201691708No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG19930.144133693583194No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCCAGGACGGTCAGTTTGGTCCCTC19790.14312121404974457No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT19770.14297657411639467No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT19640.14203641454962018No Hit
GTCCTGGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC17990.13010362004825188No Hit
TCTAAATACTGAGCGGCCCTCAGGGATCCCTGAGCGATTCTCCGGCTCCTATTATGGGAACACAGCCACTCTGAC17960.129886660148227No Hit
GGGAATAACTTGGGGGATAGATATACTTCCTGGTATCAACAGAAGCCAGGCCAATCCCCTGTGGTTGTCATTTAT17660.1277170611479782No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC17350.12547514218105446No Hit
GGCCAATCCCCTGTGGTTGTCATTTATCTAAATACTGAGCGGCCCTCAGGGATCCCTGAGCGATTCTCCGGCTC17200.1243903426809301No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG17010.12301626331410587No Hit
GACTATTACTGTCAGACGTGGGACTTCACTACTGCAGTAGTGTTCGGCGGAGGGACCAAACTGACCGTCCTGGGT16790.12142522404725675No Hit
GCCCTCAGGGATCCCTGAGCGATTCTCCGGCTCCTATTATGGGAACACAG16750.12113594418055693No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC16200.11715834601343415No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG16140.1167244262133844No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15760.11397626747973594No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT15590.11274682804626164No Hit
GAGTGGCTGTGTTCCCATAATAGGAGCCGGAGAATCGCTCAGGGATCCCT15560.11252986814623675No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15250.11028794917931303No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG15160.10963706947923839No Hit
GTAATAGTCAGCCTCATCCATAGCCTGTGTCCCGGTGATGGTCAGAGTGG14810.1071058706456148No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG14400.10414075201194148No Hit
CAGCCACTCTGACCATCACCGGGACACAGGCTATGGATGAGGCTGACTATTACTGTCAGACGTGGGACTTCACTA14320.1035621922785418No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA14260.10312827247849203No Hit
CACCTGCACTGGGAATAACTTGGGGGATAGATATACTTCCTGGTATCAAC14190.10262203271176733No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14170.1024773927784174No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)