FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686829

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686829
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1566180
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC84640.5404231952904519No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT82730.5282279176084486No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC68410.4367952598041094No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC62040.3961230509903076No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC54940.3507898198163685No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG54490.34791658685463994No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA49860.31835421215952187No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT48550.30998991175982327No Hit
GATCTATGGTGCTTCCAAGGTAGAAACAGGGGTCCCATCAGGGTTCAGTGCAAGTGGATCTGGGACAGACTTTA46790.2987523783983961No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT46540.29715613786410244No Hit
ACATATTACTGTCAACAATATGCGGATTATCCCCTCAGTTTCGGCGGAGGGACCAAGGTGGAGATCAAGCGAA45560.29089887496967143No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG45250.2889195367071473No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG44950.28700404806599494No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC43840.27991674009373124No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG42320.27021159764522595No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT40080.255909282457955No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG39930.25495153813737886No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG39880.2546322900305201No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT39470.25201445555427854No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT38590.246395688873565No Hit
ATATTACTGTCAACAATATGCGGATTATCCCCTCAGTTTCGGCGGAGGGA38570.2462679896308215No Hit
GTGCAAGTGGATCTGGGACAGACTTTACTTTCACCATCAGCAGCCTGCAGCCTGACCATGTTGCAACATATTA38570.2462679896308215No Hit
GTGCAGCCACAGTTCGCTTGATCTCCACCTTGGTCCCTCCGCCGAAACTG38520.24594874152396276No Hit
GTAATATGTTGCAACATGGTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAGTCTGTCCCAGATCCACTTGCA35470.22647460700558047No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG34060.21747181039216437No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG33700.21517322402278155No Hit
CCATAGATCAGGAGCTTAGGGGCTTCCCCTGGTTTTTGTTGATACCAATT33650.2148539759159228No Hit
GTTGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCGGGCCATTACACAATATTTAAATTGGTATCAACAAAAA33320.21274693841065526No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG31230.19940236754396046No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC30340.19371975124187513No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG30230.19301740540678594No Hit
CCCCTAAGCTCCTGATCTATGGTGCTTCCAAGGTAGAAACAGGGGTCCCA29440.18797328531841806No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC28530.18216296977358926No Hit
CAGTAATATGTTGCAACATGGTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAGTCTGTCCCAGATCCACTTGCA28400.18133292469575654No Hit
GACTTTACTTTCACCATCAGCAGCCTGCAGCCTGACCATGTTGCAACATATTACTGTCAACAATATGCGGATTAT28370.1811413758316413No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTTGGAGACAGAGTCACCATCACTT27960.17852354135539977No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA27400.17494796255858203No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATCTCCACCTTGGTCCCTCCGCCGAAACTGAGGGGATAATC27120.17316017316017315No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA26400.1685630004214075No Hit
GTAGAAACAGGGGTCCCATCAGGGTTCAGTGCAAGTGGATCTGGGACAGACTTTACTTTCACCATCAGCAGCCTG26080.16651981253751164No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT25850.16505127124596153No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA25830.164923572003218No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT25720.16422122616812884No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA25700.16409352692538534No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC25430.1623695871483482No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT25370.16198648942011776No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC24860.15873015873015872No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC24370.15560152728294321No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA24180.15438838447688005No Hit
GTATATTACTGTGCGAGATGGCATTCGTGTAGCCTGAGTTTCGACGTCAA24130.15406913637002131No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT23980.15311139204944516No Hit
GCCTGCAGCCTGACCATGTTGCAACATATTACTGTCAACAATATGCGGATTATCCCCTCAGTTTCGGCGGAGGGA23890.15253674545709944No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT23830.15215364772886897No Hit
GACCAAGGTGGAGATCAAGCGAACTGTGGCTGCACCATCTGTCTTCATCT23810.1520259484861255No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG23630.15087665530143407No Hit
TCAGGGTTCAGTGCAAGTGGATCTGGGACAGACTTTACTTTCACCATCAG23150.1478118734755903No Hit
GTCTCCAACAGATGCAGACAGGGAGGATGGAGACTGGGTCATCTCGATGTCACATCTGGCACCTGAGAGCCAGAG22980.1467264299122706No Hit
CACTTGCACTGAACCCTGATGGGACCCCTGTTTCTACCTTGGAAGCACCATAGATCAGGAGCTTAGGGGCTTCCC22980.1467264299122706No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA22910.1462794825626684No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT22820.1457048359703227No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA22790.14551328710620745No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC22250.1420654075521332No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT22160.14149076095978752No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC21890.13976682118275038No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT21770.13900062572628943No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC21650.1382344302698285No Hit
GGCTGCAGGCTGCTGATGGTGAAAGTAAAGTCTGTCCCAGATCCACTTGC21500.13727668594925233No Hit
CATCAGGGTTCAGTGCAAGTGGATCTGGGACAGACTTTACTTTCACCATCAGCAGCCTGCAGCCTGACCATGTTG21500.13727668594925233No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA21380.13651049049279138No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC21170.13516964844398474No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC20920.1335734079096911No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG20700.1321687162395127No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC20520.13101942305482128No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT20340.12987012987012986No Hit
GCTTAGGGGCTTCCCCTGGTTTTTGTTGATACCAATTTAAATATTGTGTA20300.1296147313846429No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT19880.1269330472870296No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT19550.12482600978176199No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT19460.12425136318941629No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG19360.12361286697569883No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC19260.12297437076198138No Hit
GGTAGAAACAGGGGTCCCATCAGGGTTCAGTGCAAGTGGATCTGGGACAGACTTTACTTTCACCATCAGCAGC19090.12188892719866172No Hit
GATTATCCCCTCAGTTTCGGCGGAGGGACCAAGGTGGAGATCAAGCGAACTGTGGCTGCACCATCTGTCTTCAT19050.12163352871317472No Hit
GATCTGGGACAGACTTTACTTTCACCATCAGCAGCCTGCAGCCTGACCAT18720.11952649120790715No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT18630.11895184461556142No Hit
GTCCCAGATCCACTTGCACTGAACCCTGATGGGACCCCTGTTTCTACCTT18530.11831334840184397No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCGAGATGACCCAGTCTCCATCCTCC18340.11710020559578083No Hit
ATATTTAAATTGGTATCAACAAAAACCAGGGGAAGCCCCTAAGCTCCTGA17960.1146739199836545No Hit
CTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTC17870.11409927339130878No Hit
GCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCGAGATGACCCAGTCTCCATC17700.11301382982798912No Hit
GTAATATACAGCCGTGTCTTCAGATGTCAGGCTGCTCAGCTCCATGTAGA17480.11160913815781073No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC17260.11020444648763233No Hit
GTCTGCATCTGTTGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCGGG17130.10937440140979965No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT16700.10662886769081459No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC16680.10650116844807112No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG16370.104521830185547No Hit
CTCCTGATCTATGGTGCTTCCAAGGTAGAAACAGGGGTCCCATCAGGGTTCAGTGCAAGTGGATCTGGGACAGA16350.10439413094280352No Hit
ATCTCCACCTTGGTCCCTCCGCCGAAACTGAGGGGATAATCCGCATATTG16350.10439413094280352No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA16180.10330868737948384No Hit
GGTGGAGATCAAGCGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGC16180.10330868737948384No Hit
GGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAA16140.10305328889399686No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG16020.10228709343753592No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA16010.10222324381616416No Hit
ACCCTGATGGGACCCCTGTTTCTACCTTGGAAGCACCATAGATCAGGAGC15980.10203169495204895No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA15970.10196784533067718No Hit
GATTTCAACTGCTCATCAGATGGCGGGAAGATGAAGACAGATGGTGCAGC15940.10177629646656194No Hit
GGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT15780.10075470252461403No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG15770.10069085290324228No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGAC150.00251231267.888983
GTGGTAT2950.046.026431
CGTATGC901.8189894E-1242.1799641
TCGCTAA903.6379788E-1241.69272231
TCGTATG955.456968E-1239.9120240
CGCTAAT955.456968E-1239.53896332
AACGATT501.8857297E-434.42482839
ACACTCG1103.274181E-1134.01726527
CGAGACA1103.45608E-1133.9596723
TTGAACG400.00281304633.9412410
GGCCAAG11950.033.07378470
ACGAGAC1155.638867E-1132.47901522
ATCAACG34000.032.3471033
GGGCCAA8750.031.97973469
TCCTACG552.9682662E-431.86486850
TCAACGC34450.031.8260364
CAACGCA35000.031.7138545
CGATTAA553.251523E-431.37352441
ACGATTA553.274492E-431.33588640
CTCGCTA1208.913048E-1131.24848230
Produced by FastQC (version 0.11.5)