FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686830

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686830
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1566180
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT83340.532122744512125No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC70320.44899053748611273No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC67710.4323257863080872No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC64820.41387324573165285No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC54640.34887433117521616No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT51090.32620771558824657No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT49880.3184819114022654No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA47310.3020725587097268No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC46710.29824158142742213No Hit
GTCCCAACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGG46190.2949214011160914No Hit
GATCTATGGTGCTTCCAAGGTAGAAACAGGGGTCCCATCAGGGTTCAGTGCAAGTGGATCTGGGACAGACTTTA44130.2817683791135118No Hit
ACATATTACTGTCAACAATATGCGGATTATCCCCTCAGTTTCGGCGGAGGGACCAAGGTGGAGATCAAGCGAA41810.266955266955267No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG41380.2642097332362819No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG40400.25795247034185087No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT40200.256675477914416No Hit
GTGCAAGTGGATCTGGGACAGACTTTACTTTCACCATCAGCAGCCTGCAGCCTGACCATGTTGCAACATATTA40050.2557177335938398No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT39820.25424919230228965No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT38540.24607644076670626No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG37540.23969147862953175No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT37530.23962762900815998No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG37280.23803138847386637No Hit
GTTGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCGGGCCATTACACAATATTTAAATTGGTATCAACAAAAA37040.23649899756094445No Hit
ATATTACTGTCAACAATATGCGGATTATCCCCTCAGTTTCGGCGGAGGGA36430.23260417065726802No Hit
GTGCAGCCACAGTTCGCTTGATCTCCACCTTGGTCCCTCCGCCGAAACTG36370.23222107292903754No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG35780.22845394526810459No Hit
GTAATATGTTGCAACATGGTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAGTCTGTCCCAGATCCACTTGCA34300.21900420130508627No Hit
CCATAGATCAGGAGCTTAGGGGCTTCCCCTGGTTTTTGTTGATACCAATT32310.20629812665210898No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG31990.2042549387682131No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA30400.1941028489701056No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG30350.19378360086324686No Hit
CCCCTAAGCTCCTGATCTATGGTGCTTCCAAGGTAGAAACAGGGGTCCCA29840.19052727017328788No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG29220.18656859364823966No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG28890.18446155614297208No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGCTTGATCTCCACCTTGGTCCCTCCGCCGAAACTGAGGGGATAATC28630.1828014659873067No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTTGGAGACAGAGTCACCATCACTT28430.18152447355987178No Hit
GACTTTACTTTCACCATCAGCAGCCTGCAGCCTGACCATGTTGCAACATATTACTGTCAACAATATGCGGATTAT28320.1808221277247826No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA27830.1776934962775671No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA27810.17756579703482359No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC27070.17284092505331444No Hit
AACCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTC26470.16900994777100972No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA26340.16817990269317704No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCAC25260.16128414358502854No Hit
GTAGAAACAGGGGTCCCATCAGGGTTCAGTGCAAGTGGATCTGGGACAGACTTTACTTTCACCATCAGCAGCCTG25240.16115644434228504No Hit
CAGTAATATGTTGCAACATGGTCAGGCTGCAGGCTGCTGATGGTGAAAGTAAAGTCTGTCCCAGATCCACTTGCA25070.16007100077896538No Hit
GCCTGCAGCCTGACCATGTTGCAACATATTACTGTCAACAATATGCGGATTATCCCCTCAGTTTCGGCGGAGGGA24870.15879400835153049No Hit
CAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACAT24200.15451608371962353No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT23910.15266444469984294No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC23340.14902501628165346No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA23260.14851421931067949No Hit
GTCTCCAACAGATGCAGACAGGGAGGATGGAGACTGGGTCATCTCGATGTCACATCTGGCACCTGAGAGCCAGAG23210.14819497120382077No Hit
GTATATTACTGTGCGAGATGGCATTCGTGTAGCCTGAGTTTCGACGTCAA22870.14602408407718143No Hit
GTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACC22640.14455554278563126No Hit
GGCTGCAGGCTGCTGATGGTGAAAGTAAAGTCTGTCCCAGATCCACTTGC22610.14436399392151605No Hit
GCCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTC22430.14321470073682463No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT22360.14276775338722242No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA21600.13791518216296977No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA21590.13785133254159804No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC21590.13785133254159804No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC21590.13785133254159804No Hit
CCAGGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCT21530.13746823481336756No Hit
CACTTGCACTGAACCCTGATGGGACCCCTGTTTCTACCTTGGAAGCACCATAGATCAGGAGCTTAGGGGCTTCCC21490.13721283632788056No Hit
GACCAAGGTGGAGATCAAGCGAACTGTGGCTGCACCATCTGTCTTCATCT21320.1361273927645609No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGT21270.1358081446577022No Hit
TCAGGGTTCAGTGCAAGTGGATCTGGGACAGACTTTACTTTCACCATCAG21040.13433960336615203No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT20380.13012552835561683No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA20370.13006167873424512No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC20290.12955088176327115No Hit
CATCAGGGTTCAGTGCAAGTGGATCTGGGACAGACTTTACTTTCACCATCAGCAGCCTGCAGCCTGACCATGTTG20260.1293593328991559No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCC20190.1289123855495537No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGT20090.12827388933583625No Hit
GGTGGAGATCAAGCGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGC20060.128082340471721No Hit
GGACACAGCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCT20030.12789079160760575No Hit
AACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGT19720.12591145334508166No Hit
GCTTAGGGGCTTCCCCTGGTTTTTGTTGATACCAATTTAAATATTGTGTA19610.12520910750999245No Hit
ATATTTAAATTGGTATCAACAAAAACCAGGGGAAGCCCCTAAGCTCCTGA19300.12322976924746835No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT19300.12322976924746835No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG19210.12265512265512266No Hit
TTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGC19170.12239972416963568No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT18660.11914339347967667No Hit
GCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCGAGATGACCCAGTCTCCATCCTCC18630.11895184461556142No Hit
GGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGG18600.11876029575144618No Hit
GTCCCAGATCCACTTGCACTGAACCCTGATGGGACCCCTGTTTCTACCTT18510.11818564915910049No Hit
GGTAGAAACAGGGGTCCCATCAGGGTTCAGTGCAAGTGGATCTGGGACAGACTTTACTTTCACCATCAGCAGC18450.11780255143087001No Hit
GTCTGCATCTGTTGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCGGG18410.11754715294538304No Hit
GCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGT18150.11588706278971765No Hit
GTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCA18130.11575936354697416No Hit
CTCCTGATCTATGGTGCTTCCAAGGTAGAAACAGGGGTCCCATCAGGGTTCAGTGCAAGTGGATCTGGGACAGA17510.11180068702192597No Hit
GATTATCCCCTCAGTTTCGGCGGAGGGACCAAGGTGGAGATCAAGCGAACTGTGGCTGCACCATCTGTCTTCAT17510.11180068702192597No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC17460.11148143891506725No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC17320.1105875442158628No Hit
GCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCGAGATGACCCAGTCTCCATC17050.10886360443882567No Hit
GTAATATACAGCCGTGTCTTCAGATGTCAGGCTGCTCAGCTCCATGTAGA16990.1084805067105952No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT16930.10809740898236474No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT16210.10350023624359907No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG16040.1024147926802794No Hit
GAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGG16020.10228709343753592No Hit
GATCTGGGACAGACTTTACTTTCACCATCAGCAGCCTGCAGCCTGACCAT15890.10145704835970322No Hit
CAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTA15820.10101010101010101No Hit
GTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGGCTCTCAGGTGCCAGATGTGACATCGAGATGACCCAGTCT15760.10062700328187053No Hit
ATCTCCACCTTGGTCCCTCCGCCGAAACTGAGGGGATAATCCGCATATTG15720.10037160479638356No Hit
GATCAGGAGCTTAGGGGCTTCCCCTGGTTTTTGTTGATACCAATTTAAAT15700.10024390555364007No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)