FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686831

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686831
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences913535
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTATTACTGTGCAGCATGGGATGACAACCTGCGTGGAGTCCTTTTCGG73700.8067561724509734No Hit
GTAATAAGCAGCCGCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG73080.7999693498333398No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC61300.6710197200982995No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG44010.481754940971063No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCGTGGTCCCCCCGCCGAAAAGGACTCCACGCAGGTTGTCATCCCATG43900.48055082728083764No Hit
GACCTAGGACGGTCAGCGTGGTCCCCCCGCCGAAAAGGACTCCACGCAGG42760.46807183085486603No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT41680.4562496237144718No Hit
GTCCTGGGCCCAGTCTGTGTTGACTCAGCCAGCCTCAATGTCTGGGACCC35950.3935262469418249No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG35950.3935262469418249No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC32730.35827855528250147No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC30590.3348530707635723No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCGTGGTCCCCC29620.3242349773134034No Hit
GTGTTGACTCAGCCAGCCTCAATGTCTGGGACCCCCGGGCAGAGAGTCAC29270.32040370648086824No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGTTGACTCAGCCAGCCTCAATGTCT27730.3035461148177136No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT26840.29380374041498136No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT26760.29292802136754476No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG24790.2713634398244183No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG24650.26983093149140425No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC23670.25910337316030585No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG23040.2522070856617426No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC22750.24903260411478487No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22680.24826634994827784No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC22480.24607705232968635No Hit
ACCTAGGACGGTCAGCGTGGTCCCCCCGCCGAAAAGGACTCCACGCAGGT22250.24355936006830609No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA22190.24290257078272864No Hit
CTTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT21110.23108036364233442No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG20800.2276869523335176No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGTTGACTCAGCCAG20720.22681123328608097No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA20680.22637337376236272No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA20440.22374621662005287No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20200.22111905947774305No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT19550.21400384221732066No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG19420.21258079876523614No Hit
TTTCTAGAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGTTGCTGGTACCA19160.20973471186106718No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA19000.20798327376619397No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGCCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGT18780.20557504638574328No Hit
GCATGGGATGACAACCTGCGTGGAGTCCTTTTCGGCGGGGGGACCACGCTGACCGTCCTAGGTCAGCCCAAGG17900.1959421368639406No Hit
CCCCTGAGGCCCGTTGATCATTTCTAGAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGTTGCTGGTACCAGTAAA17800.19484748805464486No Hit
CTCTTGTTCTGGAGGCATCTCCAATATCGAAAGTAATTTTGTTTACTGGTACCAGCAACTCCCAGGAACGGCCCC17370.19014049817467313No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC17300.1893742440081661No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC17090.18707548150864498No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACTTATGAACATTC16980.18587136781841967No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACTTATGAACATTCTGTAGGGGC16860.18455778924726474No Hit
GTGCAGCATGGGATGACAACCTGCGTGGAGTCCTTTTCGGCGGGGGGACC16610.18182116722402536No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT16470.1802886588910113No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA16400.17952240472450426No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCGTGGTCCCCCCGCCGAAAAGGACTC15670.17153146841664524No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA15650.17131253865478607No Hit
ATGCTGCACAGTAATAAGCAGCCGCATCCTCGGACCGGAGCCCACTGATG15490.16956110055991286No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT15440.169013776155265No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT15050.16474464579901155No Hit
GAACAAGAGATGGTGACTCTCTGCCCGGGGGTCCCAGACATTGAGGCTGG14830.16233641841856086No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG14810.1621174886567017No Hit
CTGATGGCCAGGGAGGCTGAGGTGGCAGACTTGGAGCCAGAGAATCGGTC14700.1609133749664764No Hit
ATCCCATGCTGCACAGTAATAAGCAGCCGCATCCTCGGACCGGAGCCCAC14550.15927140175253274No Hit
GGCCATCAGTGGGCTCCGGTCCGAGGATGCGGCTGCTTATTACTGTGCAGCATGGGATGACAACCTGCGTGGAGT14530.15905247199067357No Hit
GTTTGGGGGCCGTTCCTGGGAGTTGCTGGTACCAGTAAACAAAATTACTT14420.15784835830044827No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC14300.15653477972929333No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT14260.15609692020557506No Hit
ATATTGGAGATGCCTCCAGAACAAGAGATGGTGACTCTCTGCCCGGGGGT14180.15522120115813845No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC14170.15511173627720887No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT14100.15434548211070184No Hit
GTCTGGGACCCCCGGGCAGAGAGTCACCATCTCTTGTTCTGGAGGCATCTCCAATATCGAAAGTAATTTTGTTTA13970.15292243865861735No Hit
TTACTGTGCAGCATGGGATGACAACCTGCGTGGAGTCCTTTTCGGCGGGG13950.15270350889675818No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG13800.15106153568281458No Hit
CCCAGGAACGGCCCCCAAACTCCTCATCTCTAGAAATGATCAACGGGCCTCAGGGGTCCCTGACCGATTCTCTGG13590.14876277318329348No Hit
CTCTAGAAATGATCAACGGGCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGCCACCTCAGCCTCC13470.14744919461213854No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA13440.14712079996934982No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT13420.14690187020749068No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC13190.14438417794611044No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG13170.14416524818425128No Hit
GCACAGTAATAAGCAGCCGCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGGCAGACTTGGAG12840.1405529071135753No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC12840.1405529071135753No Hit
GTTCTGGAGGCATCTCCAATATCGAAAGTAATTTTGTTTACTGGTACCAGCAACTCCCAGGAACGGCCCCCAAA12750.1395677231852091No Hit
GTTGAGAACTTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG12730.13934879342334994No Hit
ATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTG12730.13934879342334994No Hit
GGCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGCCACCTCAG12630.1382541446140542No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC12540.137268960685688No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC12400.13573645235267395No Hit
GTCAGGGACCCCTGAGGCCCGTTGATCATTTCTAGAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGTTGCTGGTA12320.13486073330523735No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC12270.13431340890058946No Hit
CTCCAATATCGAAAGTAATTTTGTTTACTGGTACCAGCAACTCCCAGGAA12180.13332822497222327No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAAGTTCTCAACCCTCACCCCCCAC12170.13321876009129371No Hit
GCCCAGGACCCTGCACAGTGAGTGAGGAGGGTGAGGAGGAGAGGGAAGCCGGCCATGCTGGAGATTGTCCTGAAT12170.13321876009129371No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT12040.13179571663920922No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC12000.13135785711549094No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC11780.12894962973504026No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCGTGGTC11700.12807391068760365No Hit
GTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTC11680.12785498092574452No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA11670.1277455160448149No Hit
GTCCCTGACCGATTCTCTGGCTCCAAGTCTGCCACCTCAGCCTCCCTGGC11440.12522782378343467No Hit
GCCCCCAAACTCCTCATCTCTAGAAATGATCAACGGGCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGT11290.12358585056949105No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA11270.1233669208076319No Hit
GCCTCAATGTCTGGGACCCCCGGGCAGAGAGTCACCATCTCTTGTTCTGG11270.1233669208076319No Hit
GGATGACAACCTGCGTGGAGTCCTTTTCGGCGGGGGGACCACGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC11200.12260066664112486No Hit
GTCCGAGGATGCGGCTGCTTATTACTGTGCAGCATGGGATGACAACCTGCGTGGAGTCCTTTTCGGCGGGGGGAC11100.12150601783182911No Hit
TCCTCGGACCGGAGCCCACTGATGGCCAGGGAGGCTGAGGTGGCAGACTTGGAGCCAGAGAATCGGTCAGGGACC10910.11942618509416716No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA10640.11647063330906862No Hit
GTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCT10580.11581384402349117No Hit
GGTTGTCATCCCATGCTGCACAGTAATAAGCAGCCGCATCCTCGGACCGG10550.11548544938070245No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGG10550.11548544938070245No Hit
GGCAGAGAGTCACCATCTCTTGTTCTGGAGGCATCTCCAATATCGAAAGTAATTTTGTTTACTGGTACCAGCAA10510.11504758985698413No Hit
CATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAAGTTCTCAACCCTCACCCCCCACCA10470.11460973033326582No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG10240.11209203807188559No Hit
GTTCCTGGGAGTTGCTGGTACCAGTAAACAAAATTACTTTCGATATTGGA10220.11187310831002643No Hit
CTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACTTATGAACATTCTGTAGGGGCCACTGTCTTCT10000.10946488092957578No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG9930.10869862676306874No Hit
GGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCCC9930.10869862676306874No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGTTGACTCAGCCAGCCTCAATGTCTGGGA9920.10858916188213916No Hit
CTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTC9920.10858916188213916No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG9910.10847969700120959No Hit
GCGTGGTCCCCCCGCCGAAAAGGACTCCACGCAGGTTGTCATCCCATGCTGCACAGTAATAAGCAGCCGCATCCT9850.10782290771563213No Hit
ACCCTGCACAGTGAGTGAGGAGGGTGAGGAGGAGAGGGAAGCCGGCCATG9850.10782290771563213No Hit
GATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG9770.10694718866819553No Hit
GGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT9650.10563361009704063No Hit
ACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAGGATGCGGCTGC9590.10497682081146316No Hit
GGCTGAGGTGGCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGG9530.1043200315258857No Hit
CTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTG9490.10388217200216741No Hit
GTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTT9480.10377270712123782No Hit
CCCGTGGTGGGGGGTGAGGGTTGAGAACTTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTT9470.10366324224030826No Hit
CTCCAGAACAAGAGATGGTGACTCTCTGCCCGGGGGTCCCAGACATTGAGGCTGGCTGAGTCAACACAGACTGGG9430.10322538271658994No Hit
CAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGG9290.10169287438357588No Hit
CCTGAGGCCCGTTGATCATTTCTAGAGATGAGGAGTTTGGGGGCCGTTCC9260.10136447974078716No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGG9220.10092662021706886No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT9140.10005090116963226No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGTA150.002669692766.854175
AGGGGGG1500.066.0719170
TCGTATG2100.050.68483740
TTATAGG200.0083373850.140633
CGTATGC2150.049.665341
GCCGTCT2050.047.73108746
TTATACA2200.047.10182
TCTCGTA2050.046.626438
CACGAGA2250.046.0576221
TATACAC2250.046.0550963
GATCTCG2150.045.80735836
CTTATAC2300.045.0538941
GCCAGAG26800.044.90494570
ATCTCGT2200.044.8460937
CCCACGA2350.044.09771719
GCCCACG2350.044.09530318
GGTATCA5950.043.820381
ACGAGAC2400.043.1813822
CTGATCT2300.042.6373334
AGACCGA2450.042.32793425
Produced by FastQC (version 0.11.5)