FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686832

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686832
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences913535
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTATTACTGTGCAGCATGGGATGACAACCTGCGTGGAGTCCTTTTCGG71670.7845348016222695No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC56930.6231835671320749No Hit
GTAATAAGCAGCCGCATCCTCGGACCGGAGCCCACTGATGGCCAGGGAGG41670.45614015883354225No Hit
GTCCTGGGCCCAGTCTGTGTTGACTCAGCCAGCCTCAATGTCTGGGACCC40280.4409245403843312No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCGTGGTCCCCCCGCCGAAAAGGACTCCACGCAGGTTGTCATCCCATG39580.4332619987192609No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG39160.42866447372021876No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT39080.4277887546727821No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG39070.4276792897918525No Hit
GTGTTGACTCAGCCAGCCTCAATGTCTGGGACCCCCGGGCAGAGAGTCAC35560.38925711658557144No Hit
GACCTAGGACGGTCAGCGTGGTCCCCCCGCCGAAAAGGACTCCACGCAGG34960.38268922372979686No Hit
GTGCAGGGTCCTGGGCCCAGTCTGTGTTGACTCAGCCAGCCTCAATGTCT30430.3331016326686991No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC30130.3298176862408118No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC29690.3250012314799105No Hit
GTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCA27920.3056259475553756No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG26510.2901913993443054No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT26490.2899724695824462No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG25640.28066795470343225No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCGTGGTCCCCC25190.2757420350616014No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23540.25768032970822136No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG23480.2570235404226439No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC23170.25363012911382704No Hit
CCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGTTGACTCAGCCAG23100.25286387494732004No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC22930.2510029719715172No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT21960.24038487852134838No Hit
CCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGCCACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGT21850.23918076483112305No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA21710.23764825649810897No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG21560.23600628328416537No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA20970.22954785530932037No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG20600.22549765471492608No Hit
CTCTTGTTCTGGAGGCATCTCCAATATCGAAAGTAATTTTGTTTACTGGTACCAGCAACTCCCAGGAACGGCCCC20350.2227610326916867No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG20260.2217758487633205No Hit
GTATCAACGCAGAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGG19640.21498902614568682No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC19350.21181454459872912No Hit
CTTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT19210.21028203626571504No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT18880.20666969519503908No Hit
TTTCTAGAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGTTGCTGGTACCA18800.20579397614760245No Hit
CCCCTGAGGCCCGTTGATCATTTCTAGAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGTTGCTGGTACCAGTAAA18330.2006491267439124No Hit
ATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGC18060.19769357495881384No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT17890.19583267198301108No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA17730.19408123388813783No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACTTATGAACATTC17590.19254872555512378No Hit
ACCTAGGACGGTCAGCGTGGTCCCCCCGCCGAAAAGGACTCCACGCAGGT16980.18587136781841967No Hit
GTGCAGCATGGGATGACAACCTGCGTGGAGTCCTTTTCGGCGGGGGGACC16670.1824779565096028No Hit
GTCTGGGACCCCCGGGCAGAGAGTCACCATCTCTTGTTCTGGAGGCATCTCCAATATCGAAAGTAATTTTGTTTA16640.18214956186681408No Hit
GCATGGGATGACAACCTGCGTGGAGTCCTTTTCGGCGGGGGGACCACGCTGACCGTCCTAGGTCAGCCCAAGG16630.1820400969858845No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA16590.1816022374621662No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC16500.18061705353380003No Hit
GTTTGGGGGCCGTTCCTGGGAGTTGCTGGTACCAGTAAACAAAATTACTT16050.1756911338919691No Hit
GGCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGCCACCTCAG15970.1748154148445325No Hit
GTTCTGGAGGCATCTCCAATATCGAAAGTAATTTTGTTTACTGGTACCAGCAACTCCCAGGAACGGCCCCCAAA15940.17448702020174378No Hit
CTCTAGAAATGATCAACGGGCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGTCTGCCACCTCAGCCTCC15770.17262611722594098No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT15250.16693394341760306No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACTTATGAACATTCTGTAGGGGC15240.16682447853667348No Hit
GAGTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCT15200.16638661901295515No Hit
GGCCATCAGTGGGCTCCGGTCCGAGGATGCGGCTGCTTATTACTGTGCAGCATGGGATGACAACCTGCGTGGAGT15160.16594875948923687No Hit
CCCAGGAACGGCCCCCAAACTCCTCATCTCTAGAAATGATCAACGGGCCTCAGGGGTCCCTGACCGATTCTCTGG14950.16364999698971577No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC14850.16255534818042No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT14840.16244588329949045No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA14660.16047551544275807No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT14520.15894300710974402No Hit
CTCCAATATCGAAAGTAATTTTGTTTACTGGTACCAGCAACTCCCAGGAA14440.1580672880623074No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC14040.15368869282512437No Hit
CTGATGGCCAGGGAGGCTGAGGTGGCAGACTTGGAGCCAGAGAATCGGTC13990.15314136842047651No Hit
ATATTGGAGATGCCTCCAGAACAAGAGATGGTGACTCTCTGCCCGGGGGT13720.15018581663537794No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC13690.14985742199258922No Hit
GCCCCCAAACTCCTCATCTCTAGAAATGATCAACGGGCCTCAGGGGTCCCTGACCGATTCTCTGGCTCCAAGT13460.14733972973120898No Hit
GAACAAGAGATGGTGACTCTCTGCCCGGGGGTCCCAGACATTGAGGCTGG13410.1467924053265611No Hit
ATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTG13150.14394631842239214No Hit
TTACTGTGCAGCATGGGATGACAACCTGCGTGGAGTCCTTTTCGGCGGGG13130.143727388660533No Hit
GTCCCTGACCGATTCTCTGGCTCCAAGTCTGCCACCTCAGCCTCCCTGGC13040.1427422047321668No Hit
GGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCTGACGC12920.14142862616101187No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCGTGGTCCCCCCGCCGAAAAGGACTC12900.14120969639915276No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG12630.1382541446140542No Hit
GTTGAGAACTTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG12620.1381446797331246No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG12520.13705003092382886No Hit
GGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAA12510.13694056604289928No Hit
GCCTCAATGTCTGGGACCCCCGGGCAGAGAGTCACCATCTCTTGTTCTGG12430.13606484699546267No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA12300.1346418035433782No Hit
TAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAG12140.132890365448505No Hit
CGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCC11840.12960641902061773No Hit
GGCCTGGAAGGCAGATAGCAGCCCCGTCAAGGCGGGAGTGGAGACCACCA11790.12905909461596982No Hit
CCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTATCTGAGCCT11780.12894962973504026No Hit
GTACGGGAGCTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTC11770.12884016485411068No Hit
GGCAGAGAGTCACCATCTCTTGTTCTGGAGGCATCTCCAATATCGAAAGTAATTTTGTTTACTGGTACCAGCAA11710.12818337556853324No Hit
GTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCATGAAGGGAGCA11590.12686979699737833No Hit
GTCCGAGGATGCGGCTGCTTATTACTGTGCAGCATGGGATGACAACCTGCGTGGAGTCCTTTTCGGCGGGGGGAC11550.12643193747366002No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC11490.12577514818808255No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC11390.1246804993787868No Hit
GGCTGAGGTGGCAGACTTGGAGCCAGAGAATCGGTCAGGGACCCCTGAGG11340.12413317497413892No Hit
GTCAGGGACCCCTGAGGCCCGTTGATCATTTCTAGAGATGAGGAGTTTGGGGGCCGTTCCTGGGAGTTGCTGGTA11200.12260066664112486No Hit
GGATGACAACCTGCGTGGAGTCCTTTTCGGCGGGGGGACCACGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC11130.12183441247461783No Hit
CGCATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAAGTTCTCAACCCTCACCCCCCAC11060.12106815830811081No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCGTGGTC10980.12019243926067419No Hit
GGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG10960.11997350949881504No Hit
CTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTG10950.11986404461788547No Hit
CTTCAGCTGTGGTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTC10930.11964511485602632No Hit
ATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC10710.11723688747557566No Hit
CGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGCCAGGTCACGCAT10700.11712742259464606No Hit
GCCCAGGACCCTGCACAGTGAGTGAGGAGGGTGAGGAGGAGAGGGAAGCCGGCCATGCTGGAGATTGTCCTGAAT10660.11668956307092776No Hit
TCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGTCTGTGTTGACTCAGCCAGCCTCAATGTCTGGGA10380.11362454640489963No Hit
GGACAATCTCCAGCATGGCCGGCTTCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAGT10320.11296775711932219No Hit
CCCGTGGTGGGGGGTGAGGGTTGAGAACTTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTT10250.11220150295281517No Hit
CTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCC10210.11176364342909687No Hit
GTAGAGAAGACAGGATTCAGGACAATCTCCAGCATGGCCGGCTTCCCTCT10180.11143524878630814No Hit
GTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCA9990.10935541604864618No Hit
CTGTGCAGGGTCCTGGGCCCAGTCTGTGTTGACTCAGCCAGCCTCAATGTCTGGGACCCCCGGGCAGAGAGTCAC9960.10902702140585746No Hit
GTCTGTGTTGACTCAGCCAGCCTCAATGTCTGGGACCCCCGGGCAGAGAGTCACCATCTCTTGTTCTGGAGGCAT9710.10629039938261807No Hit
GGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTG9670.10585253985889978No Hit
CCTGAGGCCCGTTGATCATTTCTAGAGATGAGGAGTTTGGGGGCCGTTCC9640.10552414521611103No Hit
GTTCCTGGGAGTTGCTGGTACCAGTAAACAAAATTACTTTCGATATTGGA9610.10519575057332231No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG9560.10464842616867444No Hit
CATGAAGGGAGCACCGTGGAGAAGACAGTGGCCCCTACAGAATGTTCATAAGTTCTCAACCCTCACCCCCCACCA9530.1043200315258857No Hit
CCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGG9510.10410110176402657No Hit
TCCCTCTCCTCCTCACCCTCCTCACTCACTGTGCAGGGTCCTGGGCCCAG9500.10399163688309698No Hit
CTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACTTATGAACATTCTGTAGGGGCCACTGTCTTCT9460.10355377735937867No Hit
ACCTCAGCCTCCCTGGCCATCAGTGGGCTCCGGTCCGAGGATGCGGCTGC9410.10300645295473079No Hit
GATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG9340.10224019878822377No Hit
ATCCCATGCTGCACAGTAATAAGCAGCCGCATCCTCGGACCGGAGCCCAC9280.10158340950264633No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTAGC200.00839708450.0501862
ATAGAGT200.00839890150.0474434
GTGGTAT2000.045.062431
TGGTATC2000.041.708492
GGTATCA5200.039.798731
GTATCAA13400.039.108911
GTCCTAG32900.037.52397570
TATCAAC16050.032.6391642
AATGGTA2507.2759576E-1131.76591170
GCTTGGT553.95181E-430.345071
CCGCATT450.00541168329.693523
GTACATG450.005432011629.6707361
TCAACGC17550.029.6577454
CGTCCTA28750.029.43542369
CAACGCA17700.029.4080185
AACGCAG17600.029.3871336
ACGCAGA18300.028.2676777
CGCAGAG18350.028.2030098
AGAGTAC18700.027.86132411
CAGAGTA19000.027.24413910
Produced by FastQC (version 0.11.5)