FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686833

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686833
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1381262
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATGATCACTACTACTATCCCACAC67170.48629441771365606No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATGATCACTA66030.4780410957515663No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC60560.4384396298457498No Hit
CCCCTGTGCTGGTCGCCTCTGATGATAGCGACCGGCCCTCTGGGACCCCT57010.412738495665558No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG52920.3831278931875343No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG44720.3237618931093449No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC42800.3098615613837201No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT39730.28763551013493455No Hit
GCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACACTATTACTT38840.2811921271996189No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC38290.27721026134071597No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC37910.27445915402001936No Hit
GTGCTAACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGACGGCCAG34820.25208830764909196No Hit
CTATTACTTCCAATGTCGTTTCCCCCACAGGTCATCCTGGCCGTCTTTCCTGGGGCCACTGACACCGAGGGTGG34590.2504231637444598No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT33940.2457173222748472No Hit
AAGTAATAGTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGC33790.24463135885878276No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG33740.24426937105342794No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATGATCACTACTACTATCCCACACCTGACAGTAATA33720.24412457593128603No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG32460.23500248323634473No Hit
CTACTACTATCCCACACCTGACAGTAATACTCGGCCTCATCTCCGGCTTC30720.22240530760999724No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG30250.21900262223966202No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA29790.21567233443039768No Hit
CCTCTGATGATAGCGACCGGCCCTCTGGGACCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGGCCACC29780.21559993686932674No Hit
CCTATGTGCTAACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGACG29450.21321081735398498No Hit
GACTATCGACAGGGTGGAAGCCGGAGATGAGGCCGAGTATTACTGTCAGG29410.21292122710970113No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG29340.2124144441822044No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTAACTCAGC29330.21234204662113343No Hit
CTACTATCCCACACCTGACAGTAATACTCGGCCTCATCTCCGGCTTCCAC28690.20770860271259184No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTAACTCAGCCAC28480.2061882539301016No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTAACT28350.20524708563617908No Hit
GTGCTGGTCGCCTCTGATGATAGCGACCGGCCCTCTGGGACCCCTGAGCG28290.2048127002697533No Hit
GGCTCTGTGACCTCCTATGTGCTAACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGACGGCCAGGATGAC27430.19858651001765054No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT27080.1960525953801668No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC26970.19525622220838626No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG26590.19250511488768965No Hit
GAAATAGACAGCCGTGTCCTCAGCTCTCAGGCTGTCCATTTGCAGATACA26090.18888523683414155No Hit
GCACAGGCTCTGTGACCTCCTATGTGCTAACTCAGCCACCCTCGGTGTCA25840.18707529780736745No Hit
GTATTACTGTCAGGTGTGGGATAGTAGTAGTGATCATGTGGTATTCGGCG25520.18475857585309666No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG25500.18461378073095475No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGA25380.1837450099981032No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT25160.182152263654542No Hit
TAGTAGTAGTGATCATGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCC24830.17976314413920025No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA24750.17918396365063255No Hit
GGATAGTAGTAGTGATCATGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC24650.1784599880399229No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC24560.17780840999028424No Hit
CACTACTACTATCCCACACCTGACAGTAATACTCGGCCTCATCTCCGGCTTCCACCCTGTCGATAGTCAGGGTGG24400.17665004901314885No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA23900.17303017095960072No Hit
GTAGTAGTGATCATGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTA23830.17252338803210396No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG23720.17172701486032338No Hit
GTCGATAGTCAGGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGAATCGCT23580.17071344900532992No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT23560.17056865388318798No Hit
CTCCTGTGCTGCCTCTGGATTCACCTTCAGTAACTATGGCATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGG22800.16506643924179482No Hit
CAGTAATACTCGGCCTCATCTCCGGCTTCCACCCTGTCGATAGTCAGGGT22720.16448725875322712No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22610.16369088558144654No Hit
CTCACTGCACAGGCTCTGTGACCTCCTATGTGCTAACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGACGG22460.1626049221653821No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG22230.16093977826074995No Hit
CTCTGGGACCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGGCCACCCTGACTATCGACAGGGTGGAAGC21990.15920223679504686No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTA21910.15862305630647916No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA21870.1583334660621953No Hit
ATCCCACACCTGACAGTAATACTCGGCCTCATCTCCGGCTTCCACCCTGT21750.15746469532934376No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC21160.15319323922615696No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG20940.15160049288259578No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC20910.15138330019938287No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC20340.147256639218338No Hit
GCCCTCTGGGACCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGG20290.14689465141298322No Hit
GTTATATCCTATGAAGGAAGTACTAAAGACTATGCAGACTCCGTGAAGGG20030.14501231482513816No Hit
GAAGTAATAGTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTG19820.14349196604264797No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC19690.14255079774872545No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT19590.1418268221380158No Hit
GGCCAGGCCCCTGTGCTGGTCGCCTCTGATGATAGCGACCGGCCCTCTGG19510.1412476416494481No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA19420.14059606359980945No Hit
CATCAGAGGCGACCAGCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAG19070.13806214896232577No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC18680.13523864408055822No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT18650.1350214513973453No Hit
GAGTATTACTGTCAGGTGTGGGATAGTAGTAGTGATCATGTGGTATTCGG18620.13480425871413243No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC18410.1332839099316422No Hit
CCCTCGGTGTCAGTGGCCCCAGGAAAGACGGCCAGGATGACCTGTGGGGGAAACGACATTGGAAGTAATAGTGTG18360.13292192212628742No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG18250.13212554895450682No Hit
GTAATACTCGGCCTCATCTCCGGCTTCCACCCTGTCGATAGTCAGGGTGG18210.13183595871022297No Hit
GTGTCAGTGGCCCCAGGAAAGACGGCCAGGATGACCTGTGGGGGAAACGA17700.12814368309560387No Hit
GTAATAGTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGCTGGTCGCCTCTGATGATAGCGACCGGC17670.127926490412391No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC17580.12727491236275232No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG17310.12532017821383634No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA16650.1205419391831528No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA16630.12039714406101087No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG16190.11721165137388852No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC16170.11706685625174659No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT16080.11641527820210792No Hit
GGATGACCTGTGGGGGAAACGACATTGGAAGTAATAGTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCC16030.11605329039675312No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT16000.11583609771354023No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC15900.1151121221028306No Hit
GGATATAACTGCCACCCATTCCAGCCCCTTGCCTGGAGCCTGGCGGACCC15870.11489492941961772No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC15850.1147501342974758No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT15810.11446054405319195No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG15730.11388136356462422No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG15710.1137365684424823No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG15700.11366417088141134No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC15560.11265060502641787No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC15440.11178183429356632No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC15230.1102614855110761No Hit
GTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCC15140.10960990746143745No Hit
GACCAGCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACACTAT15110.10939271477822456No Hit
CGGCCACCCTGACTATCGACAGGGTGGAAGCCGGAGATGAGGCCGAGTAT14910.1079447635568053No Hit
CTGCACAGGCTCTGTGACCTCCTATGTGCTAACTCAGCCACCCTCGGTGT14890.10779996843466337No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG14880.1077275708735924No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC14790.10707599282395375No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT14750.1067864025796699No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG14700.10642441477431508No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG14700.10642441477431508No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA14690.10635201721324412No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC14620.10584523428574738No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATGATCA14560.1054108489193216No Hit
GTCCCAGAGGGCCGGTCGCTATCATCAGAGGCGACCAGCACAGGGGCCTG14220.10294933184290887No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTC14160.1025149464764831No Hit
AGGCCACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAGATAGCAGCC14080.1019357659879154No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT14040.10164617574363155No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC14010.10142898306041866No Hit
GCTTGGTCCCTCCGCCGAATACCACATGATCACTACTACTATCCCACACCTGACAGTAATACTCGGCCTCATCTC13980.10121179037720578No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG13950.10099459769399288No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC13940.10092220013292193No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC13840.1001982245222123No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)