FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686834

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686834
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1381262
Sequences flagged as poor quality0
Sequence length20-76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATGATCACTACTACTATCCCACAC62930.45559785181956786No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATGATCACTA61160.4427834835100075No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC57470.41606878347482235No Hit
CCCCTGTGCTGGTCGCCTCTGATGATAGCGACCGGCCCTCTGGGACCCCT53350.38624098831358566No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG48560.3515625565605946No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG46840.33911017605638905No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC38810.280974934516406No Hit
GTGCTAACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGACGGCCAG38800.280902536955335No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT38010.275183129630729No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC37260.2697533125504068No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC35420.25643216131334967No Hit
GCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACACTATTACTT33840.24499334666413758No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTG33820.24484855154199564No Hit
CTATTACTTCCAATGTCGTTTCCCCCACAGGTCATCCTGGCCGTCTTTCCTGGGGCCACTGACACCGAGGGTGG33440.24209744422129909No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG32120.232540966159932No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG31540.22834190761781614No Hit
GGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTAACTCAGC31030.22464963200319707No Hit
AAGTAATAGTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGC30460.2205229710221522No Hit
GCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTAACT30370.21987139297251357No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATGATCACTACTACTATCCCACACCTGACAGTAATA30200.2186406344343072No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT29370.21263163686541728No Hit
CCTCTGATGATAGCGACCGGCCCTCTGGGACCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGGCCACC29370.21263163686541728No Hit
CCTATGTGCTAACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGACG29370.21263163686541728No Hit
GGCTCTGTGACCTCCTATGTGCTAACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGACGGCCAGGATGAC29310.2121972514989915No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG28820.20864977100651436No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA28630.20727421734616602No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT28550.20669503685759832No Hit
CTACTACTATCCCACACCTGACAGTAATACTCGGCCTCATCTCCGGCTTC27190.19684896855194745No Hit
CCTCTCTCACTGCACAGGCTCTGTGACCTCCTATGTGCTAACTCAGCCAC27040.19576300513588296No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG26940.19503902952517335No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC26780.19388066854803795No Hit
GACTATCGACAGGGTGGAAGCCGGAGATGAGGCCGAGTATTACTGTCAGG26360.19083997098305752No Hit
CTACTATCCCACACCTGACAGTAATACTCGGCCTCATCTCCGGCTTCCAC25780.1866409124409417No Hit
GTATTACTGTCAGGTGTGGGATAGTAGTAGTGATCATGTGGTATTCGGCG25210.1825142514598968No Hit
GTGCTGGTCGCCTCTGATGATAGCGACCGGCCCTCTGGGACCCCTGAGCG25170.18222466121561295No Hit
CTCCTGTGCTGCCTCTGGATTCACCTTCAGTAACTATGGCATGCACTGGGTCCGCCAGGCTCCAGGCAAGGGG25010.18106630023847756No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA24370.17643285632993597No Hit
GCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGA24300.1759260734024392No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG24280.1757812782802973No Hit
GCACAGGCTCTGTGACCTCCTATGTGCTAACTCAGCCACCCTCGGTGTCA24170.17498490510851672No Hit
GGCCAGGCCCCTGTGCTGGTCGCCTCTGATGATAGCGACCGGCCCTCTGG23930.17324736364281362No Hit
GTAGTAGTGATCATGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTA23880.1728853758374588No Hit
TAGTAGTAGTGATCATGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCC23270.16846912461213007No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG22920.16593520997464636No Hit
GGATAGTAGTAGTGATCATGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCC22900.16579041485250445No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC22900.16579041485250445No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG22590.1635460904593046No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT22470.16267731972645305No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG22020.15941942947825974No Hit
GTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTCCTA22010.15934703191718877No Hit
GAAATAGACAGCCGTGTCCTCAGCTCTCAGGCTGTCCATTTGCAGATACA21990.15920223679504686No Hit
CACTACTACTATCCCACACCTGACAGTAATACTCGGCCTCATCTCCGGCTTCCACCCTGTCGATAGTCAGGGTGG21870.1583334660621953No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA21840.1581162733789824No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT21550.1560167441079245No Hit
CTCTGGGACCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGGCCACCCTGACTATCGACAGGGTGGAAGC21410.155003178252931No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC21000.15203487824902157No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG20780.15044213190546035No Hit
GTCGATAGTCAGGGTGGCCGTGTTCCCAGAGTTGGAGCCAGAGAATCGCT20780.15044213190546035No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC20670.14964575873367977No Hit
CAGTAATACTCGGCCTCATCTCCGGCTTCCACCCTGTCGATAGTCAGGGT20440.14798061482904765No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT19990.1447227245808543No Hit
CTCACTGCACAGGCTCTGTGACCTCCTATGTGCTAACTCAGCCACCCTCGGTGTCAGTGGCCCCAGGAAAGACGG19790.14327477335943506No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19380.1403064733555256No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC19360.14016167823338366No Hit
GCCCTCTGGGACCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGG19210.13907571481731923No Hit
GGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGCCTCCTCTCTCACTGCACAGGCTCTGTGACCTC19000.137555366034829No Hit
ATCCCACACCTGACAGTAATACTCGGCCTCATCTCCGGCTTCCACCCTGT18610.13473186115306146No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGT18450.13357350017592606No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT18290.13241513919879067No Hit
GTTATATCCTATGAAGGAAGTACTAAAGACTATGCAGACTCCGTGAAGGG18280.13234274163771972No Hit
GAGTATTACTGTCAGGTGTGGGATAGTAGTAGTGATCATGTGGTATTCGG18060.13074999529415854No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC17760.12857806846202965No Hit
GCACTGAACACAGAGGACTCACCATGGAGTTTGGGCTGAGCTGGGTTTTC17760.12857806846202965No Hit
GTGTCAGTGGCCCCAGGAAAGACGGCCAGGATGACCTGTGGGGGAAACGA17750.12850567090095869No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG17440.12626134650775886No Hit
GTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGAC17390.12589935870240404No Hit
GAAGTAATAGTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTG17190.12445140748098477No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGC17080.12365503430920419No Hit
CCCTCGGTGTCAGTGGCCCCAGGAAAGACGGCCAGGATGACCTGTGGGGGAAACGACATTGGAAGTAATAGTGTG17050.12343784162599129No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA17000.12307585382063649No Hit
GTATCAACGCAGAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTT16770.12141070991600435No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC16770.12141070991600435No Hit
CATCAGAGGCGACCAGCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAG16740.12119351723279147No Hit
GGATGACCTGTGGGGGAAACGACATTGGAAGTAATAGTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCC16710.12097632454957857No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA16610.12025234893886895No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT16530.11967316845030124No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTCTGTAGGGGC16480.11931118064494642No Hit
GTAATAGTGTGCACTGGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGCTGGTCGCCTCTGATGATAGCGACCGGC16290.11793562698459814No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA16260.11771843430138525No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG16200.11728404893495947No Hit
GTAATACTCGGCCTCATCTCCGGCTTCCACCCTGTCGATAGTCAGGGTGG16140.1168496635685337No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16060.116270483079966No Hit
GTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGGC16030.11605329039675312No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA16010.1159084952746112No Hit
GCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGC16000.11583609771354023No Hit
GGTACCAGCAGAAGCCAGGCCAGGCCCCTGTGCTGGTCGCCTCTGATGAT15860.11482253185854675No Hit
GACCAGCACAGGGGCCTGGCCTGGCTTCTGCTGGTACCAGTGCACACTAT15850.1147501342974758No Hit
GTTGAGAACCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAG15630.1131573879539146No Hit
GAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAATACCACATGATCA15420.1116370391714244No Hit
GAGTACGGGAGTCTGGGCCTAAGGAAGCAGCACTGGTGGTGCCTCAGCCATGGCCTGGACCGTTCTCCTCCTCGG15340.1110578586828567No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTT15320.11091306356071477No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC15120.10946511233929553No Hit
GGGTGGAAGCCGGAGATGAGGCCGAGTATTACTGTCAGGTGTGGGATAGT15030.10881353428965684No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG14990.108523944045373No Hit
GTGTGGGATAGTAGTAGTGATCATGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGG14980.10845154648430202No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG14790.10707599282395375No Hit
GGATATAACTGCCACCCATTCCAGCCCCTTGCCTGGAGCCTGGCGGACCC14720.106569209896457No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC14720.106569209896457No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG14620.10584523428574738No Hit
GTATTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCC14580.10555564404146353No Hit
CTGGGACCCCTGAGCGATTCTCTGGCTCCAACTCTGGGAACACGGCCACC14470.10475927086968295No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC14470.10475927086968295No Hit
AGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGC14390.10418009038111525No Hit
GTCCCAGAGGGCCGGTCGCTATCATCAGAGGCGACCAGCACAGGGGCCTG14200.10280453672076695No Hit
GCTTGGTCCCTCCGCCGAATACCACATGATCACTACTACTATCCCACACCTGACAGTAATACTCGGCCTCATCTC14160.1025149464764831No Hit
CCTTGGGGCTGGTCGGGGATGCTGAGGAGACGGTGACCAGGGTTCCCTGG14100.10208056111005732No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC14090.10200816354898636No Hit
GTCTATTTCTGTGCGAAGCCTGTCCCCTTTTTTCACGGTGACTATTTTGA13950.10099459769399288No Hit
CTGCACAGGCTCTGTGACCTCCTATGTGCTAACTCAGCCACCCTCGGTGT13900.10063260988863808No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG13820.10005342940007038No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAAA352.373143E-548.9280929
GGTATCA7550.043.048771
GTATCAA19300.038.6441081
TATCAAC21850.034.270872
CGGGACT400.0027019334.22112317
ATCAACG22600.032.9798773
TCAACGC22750.032.612144
TGGTATC2450.032.09922
CCAATCC552.8496914E-432.08673559
CAACGCA23400.031.854675
AACGCAG23300.031.8515536
AATGGTA4100.031.13943570
CCTCGAC450.004811932330.4165719
ACGCAGA24750.029.992027
AGAGTAC25100.029.73054711
CAGAGTA25250.029.6841210
CGCAGAG25100.029.580238
GCAGAGT25600.029.00889
ACGGGAC605.680453E-428.5227716
CACATCG550.0076878227.63473968
Produced by FastQC (version 0.11.5)