FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686835

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686835
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences878778
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC35460.4035148808914197No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCCTGCTCCCTCCGCCGAACACCCAGTTACCTCTACTGTCTGCTGATTGA32170.3660765289982225No Hit
GACCTAGGACGGTCAGCCTGCTCCCTCCGCCGAACACCCAGTTACCTCTACTGTCTGCTGATTGACAGTAATAGT29870.33990382098778077No Hit
GTAATAGACAGCCGTGTCTTCGTCTCTCAGACTGTTCATTTGCAGATACA26310.29939302076292307No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG23870.271627191395324No Hit
CCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATGCATTGTCAAAG22350.25433044523190157No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG22270.2534200901706688No Hit
GTCTTTATATATGACCAACACAGGGGCCTGGCCTGGCCTCTGCTGGTACC22110.2515993800482033No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT21080.23987855863483154No Hit
CCTCTACTGTCTGCTGATTGACAGTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGTGA20790.23657852153786282No Hit
GGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCAC20650.23498540018070546No Hit
ACACAGGGGCCTGGCCTGGCCTCTGCTGGTACCAATAAGCATATTGCTTT20010.22770255969084344No Hit
GGCCAGGCCAGGCCCCTGTGTTGGTCATATATAAAGACAGTGAGAGGGCC19950.22701979339491887No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATG19890.22633702709899428No Hit
GTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGTGA19750.22474390574183695No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC19280.2193955697570945No Hit
GTCTATTACTGTGCACGCAACCGGGAGACTGGTATTTCGTTCGGGGGGAT19150.21791624278259128No Hit
CAGTAGAGGTAACTGGGTGTTCGGCGGAGGGAGCAGGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGT19030.21655071019074215No Hit
CTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGG18910.215185177598893No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC18600.21165755173661607No Hit
GACTATTACTGTCAATCAGCAGACAGTAGAGGTAACTGGGTGTTCGGCGGAGGGAGCAGGCTGACCGTCCTAGGT18550.2110885798233456No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCCTGCTCCCTC18160.20665059889983592No Hit
ATATAAAGACAGTGAGAGGGCCTCAGGGATCCCTGAGCGATTCTCTGGCT18090.20585403822125725No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA18010.20494368316002448No Hit
GGCCAGGCCCCTGTGTTGGTCATATATAAAGACAGTGAGAGGGCCTCAGG17220.19595392693035102No Hit
TATATAAAGACAGTGAGAGGGCCTCAGGGATCCCTGAGCGATTCTCTGGC17170.19538495501708053No Hit
GTCCAGGAGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTAC16980.19322286174665274No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG16630.18924005835375943No Hit
CCCCTGTGTTGGTCATATATAAAGACAGTGAGAGGGCCTCAGGGATCCCT16460.18730555384863984No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT16460.18730555384863984No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT16440.18707796508333163No Hit
GTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATGC16050.18263998415982194No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG16040.18252618977716784No Hit
GTGTTGGTCATATATAAAGACAGTGAGAGGGCCTCAGGGATCCCTGAGCG15820.18002271335877776No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG15720.1788847695322368No Hit
ACCTAGGACGGTCAGCCTGCTCCCTCCGCCGAACACCCAGTTACCTCTAC15580.17729164817507947No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15290.17399161107811073No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15080.17160192904237476No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC14500.16500185484843727No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG14480.16477426608312906No Hit
CAGTAATAGTGATGGGACTGACAAAACCTACGCGGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAACGC14230.1619294065167767No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG13840.157491425593267No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA13680.1556707154708015No Hit
CAGTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGT13390.15237067837383275No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13370.15214308960852455No Hit
TCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCA13020.14816028621563126No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT12780.14542922103193298No Hit
GGCCTGGCCTGGCCTCTGCTGGTACCAATAAGCATATTGCTTTGACAATGCATCTCCAGAGCAGGTGATCCTGGC12700.1445188659707002No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12600.1433809221441593No Hit
GGCCTGGCCTCTGCTGGTACCAATAAGCATATTGCTTTGACAATGCATCTCCAGAGCAGGTGATCCTGGCCGT12520.14247056708292652No Hit
GTAATAGTGATGGGACTGACAAAACCTACGCGGACTCCGTGAAGGGCCGA12520.14247056708292652No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA12510.14235677270027242No Hit
CTATTACTGTCAATCAGCAGACAGTAGAGGTAACTGGGTGTTCGGCGGAG12300.13996709066453644No Hit
CTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGT12180.13860155807268731No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC11950.13598428727164313No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT11530.13120492320017116No Hit
GATCAGGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGG11330.12892903554708926No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA11270.12824626925116467No Hit
GACCAACACAGGGGCCTGGCCTGGCCTCTGCTGGTACCAATAAGCATATT11060.1258565872154287No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC10970.12483243777154185No Hit
GCTCTGGAGATGCATTGTCAAAGCAATATGCTTATTGGTACCAGCAGAGG10930.12437726024092546No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC10730.12210137258784357No Hit
GTAGAGGTAACTGGGTGTTCGGCGGAGGGAGCAGGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA10620.12084963437864853No Hit
CTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGT10510.11959789616945349No Hit
GTCCTGGGGACACTGACACCGAGGGTGGCTGTGTCAGCTCATAGGAGGCC10130.1152737096285979No Hit
ACGTGACTGTTGTCCGTGAGCTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGCCCTCTCA10090.11481853209798151No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT9850.11208746691428326No Hit
CCACTGATGGTCAACGTGACTGTTGTCCGTGAGCTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGCCCTCTCA9820.11174608376632096No Hit
GCAATATGCTTATTGGTACCAGCAGAGGCCAGGCCAGGCCCCTGTGTTGGTCATATATAAAGACAGTGAGAGGGC9770.11117711185305047No Hit
CCCAGTTACCTCTACTGTCTGCTGATTGACAGTAATAGTCAGCCTCGTCT9740.1108357287050882No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT9700.11038055117447182No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCCTGCTCCCTCCGCCGAACAC9570.1089012241999686No Hit
CTATTACTGTGTGAGACCCACACCAGCCCCTTCCCTGGAGCCTGGCGGAC9520.10833225228669811No Hit
CTGCTGGTACCAATAAGCATATTGCTTTGACAATGCATCTCCAGAGCAGGTGATCCTGGCCGTCTGTCCTGGGGA9510.10821845790404402No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC9440.10742189722546536No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA9410.10708051407750308No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG9370.1066253365468867No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA9330.10617015901627032No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG9280.10560118710299984No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG9230.10503221518972938No Hit
CAGTCACGTTGACCATCAGTGGAGTCCAGGCAGAAGACGAGGCTGACTAT9200.1046908320417671No Hit
GTACCAGCAGAGGCCAGGCCAGGCCCCTGTGTTGGTCATATATAAAGACA9140.10400806574584252No Hit
GGATCACCTGCTCTGGAGATGCATTGTCAAAGCAATATGCTTATTGGTACCAGCAGAGGCCAGGCCAGGCCC9090.10343909383257205No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT9060.10309771068460977No Hit
TCAGTGGAGTCCAGGCAGAAGACGAGGCTGACTATTACTGTCAATCAGCA9040.10287012191930156No Hit
ACCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAAAAGCTACAGCTGC9020.10264253315399338No Hit
CTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCA8990.1023011500060311No Hit
CTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGG8900.10127700056214424No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCCGT300.002497267467.9835670
AGGGGGG1500.058.91908370
ATAGCGG200.00812350450.4712377
CGAGACG1150.049.82766323
CGTAGAG1200.047.88252328
AATGGTA2700.047.84028270
GACGTAG1200.047.80055626
CGTATGC950.047.27600541
TCTCGTA950.046.9332538
GTCTTTA4200.046.4682271
TCGTATG1050.045.9537240
ACGTAGA1250.045.91211327
CCCACGA1250.045.81011619
ATGCCGT1000.045.1947444
AGACGTA1300.044.10597625
CACGAGA1300.044.06324821
CTTATAC1400.043.263521
ATCTCGT1200.042.7873437
CCGTCTT1150.042.60588547
GGTATCA5150.041.816741
Produced by FastQC (version 0.11.5)