FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686836

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686836
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences878778
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC33390.3799594436820221No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCCTGCTCCCTCCGCCGAACACCCAGTTACCTCTACTGTCTGCTGATTGA29760.3386520827785857No Hit
GACCTAGGACGGTCAGCCTGCTCCCTCCGCCGAACACCCAGTTACCTCTACTGTCTGCTGATTGACAGTAATAGT28440.3236312242682452No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG25770.29324812409960194No Hit
GTAATAGACAGCCGTGTCTTCGTCTCTCAGACTGTTCATTTGCAGATACA23360.26582367787996514No Hit
CCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATGCATTGTCAAAG21980.25012005307370005No Hit
GGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCAC21680.2467062215940772No Hit
GTCTTTATATATGACCAACACAGGGGCCTGGCCTGGCCTCTGCTGGTACC21470.24431653955834126No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT19900.22645082148164838No Hit
GGCCAGGCCAGGCCCCTGTGTTGGTCATATATAAAGACAGTGAGAGGGCC19870.2261094383336861No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGG19670.2238335506806042No Hit
CTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGG19100.2173472708693208No Hit
CCTCTACTGTCTGCTGATTGACAGTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGTGA19030.21655071019074215No Hit
GTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGTGA18710.21290928994581113No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC18560.2112023742059997No Hit
GTCCAGGAGGCAGAACTCTGGGTGTCTCACCATGGCCTGGATCCCTCTAC18290.20812992587433912No Hit
ATATAAAGACAGTGAGAGGGCCTCAGGGATCCCTGAGCGATTCTCTGGCT18280.20801613149168505No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCCTGCTCCCTC17950.20426091686409992No Hit
CAGTAGAGGTAACTGGGTGTTCGGCGGAGGGAGCAGGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGT17720.20164364606305574No Hit
ACACAGGGGCCTGGCCTGGCCTCTGCTGGTACCAATAAGCATATTGCTTT17660.20096087976713117No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTC17560.19982293594059025No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAG17500.19914016964466566No Hit
GTCTATTACTGTGCACGCAACCGGGAGACTGGTATTTCGTTCGGGGGGAT17480.19891258087935745No Hit
CCTATGAGCTGACACAGCCACCCTCGGTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATG17460.19868499211404927No Hit
GACTATTACTGTCAATCAGCAGACAGTAGAGGTAACTGGGTGTTCGGCGGAGGGAGCAGGCTGACCGTCCTAGGT17030.1937918336599232No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT16970.19310906736399863No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA16720.19026420779764627No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG16680.18980903026702992No Hit
GTGTTGGTCATATATAAAGACAGTGAGAGGGCCTCAGGGATCCCTGAGCG16350.18605381563944476No Hit
GGCCAGGCCCCTGTGTTGGTCATATATAAAGACAGTGAGAGGGCCTCAGG15980.18184342348124327No Hit
CCCCTGTGTTGGTCATATATAAAGACAGTGAGAGGGCCTCAGGGATCCCT15920.1811606571853187No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT15850.18036409650674004No Hit
TATATAAAGACAGTGAGAGGGCCTCAGGGATCCCTGAGCGATTCTCTGGC15750.1792261526801991No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGG15470.17603990996588445No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15270.17376402231280255No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG14980.1704639852158338No Hit
GTGTCAGTGTCCCCAGGACAGACGGCCAGGATCACCTGCTCTGGAGATGC14810.1685294807107142No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA13910.15828798627184568No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG13890.1580603975065375No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG13590.15464656602691465No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTG13370.15214308960852455No Hit
CAGTAATAGTGATGGGACTGACAAAACCTACGCGGACTCCGTGAAGGGCCGATTCACCATCTCCAGAGACAACGC13280.15111894016463773No Hit
TCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCA12960.14747751991970667No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12960.14747751991970667No Hit
CTCTACTTCTCCCCCTCCTCACTCTCTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGT12950.14736372553705257No Hit
GGCCTGGCCTGGCCTCTGCTGGTACCAATAAGCATATTGCTTTGACAATGCATCTCCAGAGCAGGTGATCCTGGC12830.14599819294520347No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC12820.14588439856254937No Hit
ACCTAGGACGGTCAGCCTGCTCCCTCCGCCGAACACCCAGTTACCTCTAC12600.1433809221441593No Hit
GGCCTGGCCTCTGCTGGTACCAATAAGCATATTGCTTTGACAATGCATCTCCAGAGCAGGTGATCCTGGCCGT12550.1428119502308888No Hit
GGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCCTGCTCCCTCCGCCGAACAC12430.14144641763903967No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTC12250.13939811875126595No Hit
GTAATAGTGATGGGACTGACAAAACCTACGCGGACTCCGTGAAGGGCCGA11860.13496013782775626No Hit
GGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA11820.1345049602971399No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT11720.13336701647059895No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC11670.13279804455732847No Hit
GACTTCCACTGCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTT11440.13018077375628428No Hit
CAGTAATAGTCAGCCTCGTCTTCTGCCTGGACTCCACTGATGGTCAACGT11430.13006697937363018No Hit
CTATTACTGTCAATCAGCAGACAGTAGAGGTAACTGGGTGTTCGGCGGAG11310.12870144678178108No Hit
GACCAACACAGGGGCCTGGCCTGGCCTCTGCTGGTACCAATAAGCATATT10510.11959789616945349No Hit
GTAGAGGTAACTGGGTGTTCGGCGGAGGGAGCAGGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCA10430.11868754110822073No Hit
GCTCTGGAGATGCATTGTCAAAGCAATATGCTTATTGGTACCAGCAGAGG10430.11868754110822073No Hit
CTGCACAGGCTCTGAGGCCTCCTATGAGCTGACACAGCCACCCTCGGTGT10310.11732200851637159No Hit
GTCCTGGGGACACTGACACCGAGGGTGGCTGTGTCAGCTCATAGGAGGCC10220.11629785907248473No Hit
CTCTGTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC10220.11629785907248473No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA10210.11618406468983065No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA10200.11607027030717655No Hit
ACGTGACTGTTGTCCGTGAGCTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGCCCTCTCA10040.11424956018471104No Hit
CCCAGTTACCTCTACTGTCTGCTGATTGACAGTAATAGTCAGCCTCGTCT9990.11368058827144058No Hit
GGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGC9890.11254264444489961No Hit
GCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGT9740.1108357287050882No Hit
GCAATATGCTTATTGGTACCAGCAGAGGCCAGGCCAGGCCCCTGTGTTGGTCATATATAAAGACAGTGAGAGGGC9700.11038055117447182No Hit
CCCCTGGGATCCTGCAGCTCTAGTCTCCCGTGGTGGGGGGTGAGGGATGAGAACCTATGAACATTCTGTAGGGGC9590.10912881296527678No Hit
GATCAGGACTGCACACAGAGAACTCACCATGGAGTTTGGGCTGAGCTGGG9570.1089012241999686No Hit
TTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAG9420.10719430846015718No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC9410.10708051407750308No Hit
CCACTGATGGTCAACGTGACTGTTGTCCGTGAGCTGGAGCCAGAGAATCGCTCAGGGATCCCTGAGGCCCTCTCA9380.1067391309295408No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC9140.10400806574584252No Hit
GGATCACCTGCTCTGGAGATGCATTGTCAAAGCAATATGCTTATTGGTACCAGCAGAGGCCAGGCCAGGCCC8990.1023011500060311No Hit
CTATTACTGTGTGAGACCCACACCAGCCCCTTCCCTGGAGCCTGGCGGAC8930.10161838371010652No Hit
CTGCTGGTACCAATAAGCATATTGCTTTGACAATGCATCTCCAGAGCAGGTGATCCTGGCCGTCTGTCCTGGGGA8880.10104941179683606No Hit
GGTAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGG8840.10059423426621969No Hit
CTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGT8830.10048043988356559No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)