FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686837

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686837
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1563189
Sequences flagged as poor quality0
Sequence length20-76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATTG47740.30540133022942206No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC47460.3036101200814489No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC39370.25185694116322466No Hit
GTCTCCAGCCACCCTGTCTTTCTCTCCAGGGGAAAGAGTCACCCTCTCCT37290.2385508086354241No Hit
GTCCCAGACCCACTGCCACTGAACCTGCCTGGGATGCCAGTGGCCCTGTT36780.23528824729447304No Hit
GTAGTAAACAGCCGTGTCCGTGTCTTCAAGACTATTCATTTGCAGAAACA35300.22582042222661497No Hit
GTCTGGGACAGACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATT34810.22268580446766195No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT33740.21584082283076456No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTGGCAGGTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT33320.21315400760880482No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC33080.21161868462482783No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG32410.2073325746278921No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA31580.20202291597497166No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31120.1990802135890158No Hit
GTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCA30200.19319480881710402No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG29380.18794912195518265No Hit
GTTTATTACTGTCGGCAGCGTGGCAACTGGCCTCCGATCACCTTCGGCCA27880.17835335330532648No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA27600.17656214315735333No Hit
GTTTACTACTGTGTCAGAGACCTTGACTACTTAGTCCCTAGGGAAGTCTGGGGCCAAGGGACCACGGTCACCGT27110.17342752539840034No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC27020.17285177927940895No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG26480.16939730256546073No Hit
GCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCGGCAGCGTGGCAAC26250.16792595137248278No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT25680.16427955928553745No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC25520.16325601062955278No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG24610.15743457764864005No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT23990.1534683266066995No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA23940.15314846765170428No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG23820.1523808061597158No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGCCTGGGATGCCA23690.15154917287672828No Hit
ACCATATACTACGCAGACTCTGTGAGGGGCCGGTTCACCGTCTCCAGAGACAACGCCAAGAAGTCTCTGTTTCTG23530.15052562422074361No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT22940.1467512885518002No Hit
GACTTCACTCTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCGGCAGCGTGGCAACTGG22840.1461115706418098No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT22570.1443843322848357No Hit
GCGTAGTACAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGA22360.14304092467385582No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC22350.14297695288285678No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG22270.14246517855486446No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT21900.14009822228789995No Hit
GTCTTTCTCTCCAGGGGAAAGAGTCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGGCAGGTACTTAGCCTGGTA21400.13689963273794786No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT21080.13485253542597855No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC20570.13158997408502748No Hit
CTCCTGTGCAGCCTCTGGATTCGTCTTCAGCAGCTATAGCATGAACTGGGTCCGCCAGGTTCCAGGGAAGGGG20440.13075834080203994No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG20130.12877521528106967No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG19880.12717592050609364No Hit
GTCAGAGTGTTGGCAGGTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATG19650.1257045693131157No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19550.12506485140312526No Hit
CCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCA19400.12410527453813966No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC19290.12340158483715022No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA19280.12333761304615116No Hit
TCCTTGGGGCTGAAGCCACGTGCCAGGCACGTCAGCGTCACCAGCTCGTT19160.12256995155416267No Hit
CTGCTACTCTGGCTCCCAGATATTGCCGGAGAAATTGTGTTGACACAGTC19070.12199420543517131No Hit
GAGTTGGGGCTGTGCTGGGTTTTCCTTGTAGCTATTTTAGAAGGTGTCCA19030.12173831827117514No Hit
TCTATGATGCATCCAACAGGGCCACTGGCATCCCAGGCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCACT18920.12103462857018568No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC18840.12052285424219336No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC18670.11943533379520967No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC18530.11853972872122309No Hit
GCACTGAACACAGAGGAGACACCATGGAGTTGGGGCTGTGCTGGGTTTTC18390.11764412364723652No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG18210.11649263140925377No Hit
CTGCAGGAGAGGGTGACTCTTTCCCCTGGAGAGAAAGACAGGGTGGCTGG17990.11508525200727487No Hit
GTGTTGACACAGTCTCCAGCCACCCTGTCTTTCTCTCCAGGGGAAAGAGT17720.11335801365030077No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT17530.11214254962131898No Hit
CCATATACTACGCAGACTCTGTGAGGGGCCGGTTCACCGTCTCCAGAGAC17380.11118297275633338No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG17290.11060722663734199No Hit
GAGTCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGGCAGGTACTTAGCCTGGTACCAACAGAAACCTGGCCAGG17080.10926381902636215No Hit
CATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGGCAGGTTCAGTGGCAGTGGGTCTGGGACAGACTTCA17070.10919984723536309No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCC17000.10875204469836981No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG16740.10708877813239473No Hit
GTACTACGCCTCCCCCCGACTCCACCAACTGCATCTCACACTGGACACCT16720.10696083455039665No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC16560.10593728589441201No Hit
ACAGAAACCTGGCCAGGCTCCCAGGCTCCTCATCTATGATGCATCCAACAGGGCCACTGGCATCCCAGGCAGGTT16520.10568139873041583No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC16200.10363430141844651No Hit
TCATAGATGAGGAGCCTGGGAGCCTGGCCAGGTTTCTGTTGGTACCAGGC16070.10280266813545899No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG16000.1023548655984657No Hit
GAGTTTATTCAGGGGTGGGGACAGGCGGGCGGCTCAGTAGCAGGTGCCGT15680.10030776828649637No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA15680.10030776828649637No Hit
GCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGCCTGGGATGCCAGTGG15670.10024379649549735No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAA200.006315001353.7921353
CGCGTAT200.00757538451.3729239
CGTATGC1400.046.73148341
TCGTATG1500.045.7773640
ACGAGAC3300.043.58545722
AAACGAC358.999555E-442.80327263
GTGGTAT3050.042.735671
TGGTATC3150.041.3789832
ATGCCGT1600.041.2119444
GTATGCC1800.040.2629142
ATCTCCG3750.040.10450410
CGAGACT3600.039.95973223
TTATACA3600.039.920112
TATACAC3800.039.578083
CACGAGA3650.039.39846421
GGTATCA8150.039.367641
CTTATAC3600.038.9917341
CCGTCTT1800.038.97384347
GTATCAA21950.037.608691
ATCTCGT2000.037.46470637
Produced by FastQC (version 0.11.5)