FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686838

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686838
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1563189
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT55390.3543397503436884No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41550.2658027916010156No Hit
GTATATGGTGTCGCTGCCACTACTGATGTACGAAATCCACTCCAGCCCCTTCCCTGGAACCTGGCGGACCCAGTT41060.2626681738420626No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC40500.2590857535461163No Hit
GTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATATTG40030.25607907936916136No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC39560.2530724051922065No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC39450.252368715491217No Hit
GTAGTAAACAGCCGTGTCCGTGTCTTCAAGACTATTCATTTGCAGAAACA35210.22524467610762358No Hit
GTCCCAGACCCACTGCCACTGAACCTGCCTGGGATGCCAGTGGCCCTGTT34890.22319757879565427No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT33550.21462535880178274No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG32060.20509356194292563No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC31380.20074348015499088No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA29520.1888447270291692No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG27490.1758584534563639No Hit
GTAATAAACTGCAAAATCTTCAGGCTCTAGGCTGCTGATGGTGAGAGTGAAGTCTGTCCCAGACCCACTGCCA26750.17112454092243484No Hit
GTCTCCAGCCACCCTGTCTTTCTCTCCAGGGGAAAGAGTCACCCTCTCCT26690.1707407101764406No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT26590.1701009922664502No Hit
GTTTACTACTGTGTCAGAGACCTTGACTACTTAGTCCCTAGGGAAGTCTGGGGCCAAGGGACCACGGTCACCGT26550.169845105102454No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC26420.1690134718194665No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC26060.166710487343501No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT25710.16447147465853457No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT25080.160441251825595No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC24890.1592257877966132No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT24700.1580103237676314No Hit
GAGTGAAGTCTGTCCCAGACCCACTGCCACTGAACCTGCCTGGGATGCCA24150.15449187526268415No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG23910.15295655227870716No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG23850.15257272153271292No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT22910.14655937317880308No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG22490.14387255795684334No Hit
ACCATATACTACGCAGACTCTGTGAGGGGCCGGTTCACCGTCTCCAGAGACAACGCCAAGAAGTCTCTGTTTCTG22400.14329681183785198No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG22190.1419534042268721No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT21720.1389467300499172No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA21600.13817906855792872No Hit
GTTCAAGGACCACCTGACCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG21430.13709154811094498No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAGCCCCAGCTCAGCTTCTCT21310.1363238866189565No Hit
GTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCCCTTGGCCGAAGGTG20890.13363707139699676No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG20770.13286940990500828No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG20670.13222969199501786No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19980.12781563841608404No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG19870.1271119487150946No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA19780.12653620259610324No Hit
CTCCTGCAGGGCCAGTCAGAGTGTTGGCAGGTACTTAGCCTGGTACCAACAGAAACCTGGCCAGGCTCCCAGGCT19750.12634428722310612No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19730.126216343641108No Hit
GGACACGACTGGAGATTAAACGAACTGTGGCTGCACCATCTGTCTTCATC18740.11988313633220296No Hit
GTCGTGTCCCTTGGCCGAAGGTGATCGGAGGCCAGTTGCCACGCTGCCGA18740.11988313633220296No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA18480.11821986976622788No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTAATCTCCAGTCGTGTCC18170.11623674424525762No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC17950.11482936484327871No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC17750.11354992902329789No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG17550.11227049320331707No Hit
CCTTCGGCCAAGGGACACGACTGGAGATTAAACGAACTGTGGCTGCACCA17330.11086311380133816No Hit
GCGTAGTACAGCCTGGGGGGTCCCTGAGACTCTCCTGTGCAGCCTCTGGA17110.10945573439935927No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG17060.10913587544436405No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA16890.10804835499738036No Hit
CTCCTGTGCAGCCTCTGGATTCGTCTTCAGCAGCTATAGCATGAACTGGGTCCGCCAGGTTCCAGGGAAGGGG16570.10600125768541105No Hit
CTGCTACTCTGGCTCCCAGATATTGCCGGAGAAATTGTGTTGACACAGTC16450.10523359619342255No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA16400.10491373723842735No Hit
GTCTTTCTCTCCAGGGGAAAGAGTCACCCTCTCCTGCAGGGCCAGTCAGAGTGTTGGCAGGTACTTAGCCTGGTA16270.1040821039554398No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT16240.10389018858244269No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG16190.10357032962744749No Hit
GGCCGAAGGTGATCGGAGGCCAGTTGCCACGCTGCCGACAGTAATAAACTGCAAAATCTTCAGGCTCTAGGCTG16150.10331444246345133No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC16140.10325047067245227No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC15880.10158720410647722No Hit
CTGCAGGAGAGGGTGACTCTTTCCCCTGGAGAGAAAGACAGGGTGGCTGG15790.10101145798748583No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC15780.1009474861964868No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC15720.10056365545049256No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA15710.1004996836594935No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGGTAT3000.042.2453271
GGTATCA8550.041.7382281
TGGTATC3050.041.5408172
GTATCAA22100.036.973461
AATGGTA6050.035.49643370
TCTGGAG17650.034.4741170
AACGATC552.3677056E-433.1161157
TATCAAC24850.032.604022
ATCAACG25000.032.5386433
TCAACGC25500.031.9006294
CAACGCA25950.031.4809475
AACGCAG26150.031.2441716
GGTACGT550.005359868529.75289768
ATAGTCA704.1809515E-529.21815337
GAATACG803.585501E-629.1938369
CGCAGAG28200.028.9858598
AGAGTAC28400.028.90476411
GAAAACG1000.00646235328.63379170
ATACTGA704.8554655E-528.584326
GAGTTTA4450.028.4799921
Produced by FastQC (version 0.11.5)