FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686839

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686839
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences622872
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGTTGGCTCAATGTGGACGTTCGGCCAGGGGA34160.5484272852207195No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26430.4243247408777405No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC23000.3692572470748404No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGGGTGAAGTCTCTCCCAGACCCACTGCCA22670.36395920831246226No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA19360.31081827405951784No Hit
CATCTATGGTGTGTCTACCAGGTCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGAGAGACTTCAC16390.2631359251981145No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC16320.2620120987939737No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG15830.25414531396498796No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15320.2459574358776763No Hit
GTGTATTACTGTCAGCAGTATGTTGGCTCAATGTGGACGTTCGGCCAGGGGACCAAGGTGGAGATCAAACGAA14930.2396961173403203No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAAGTCTCTTCTGGTACCAGGCCAAGTAATTGTTGCTAACAGTCTGA14850.23841174430701656No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT14210.2281367600405862No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC13860.2225176280198821No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG13250.21272428364094068No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA13090.21015553757433308No Hit
CTCCTGCAGGGCCAGTCAGACTGTTAGCAACAATTACTTGGCCTGGTACC12270.1969907139829692No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG12250.19666962072464328No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG12190.19570634094966544No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT12020.19297704825389486No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG12000.1926559549955689No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11980.19233486173724298No Hit
GTCTCCAGGCACCCTGTCTCTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGACTGTTAGCAA11960.19201376847891702No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGGGTGAAGTCTCTCCCAGACCCACTGCCA11620.1865551830873759No Hit
CTCCAGGCACCCTGTCTCTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC10690.1716243465752193No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10490.1684134139919598No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC10440.16761068084614497No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10310.16552357466702627No Hit
TCTATGGTGTGTCTACCAGGTCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGAGAGACTTCACC9620.15444585725478108No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT9300.14930836512156592No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC9170.14722125894244725No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCCTGGCCGAACGTC9170.14722125894244725No Hit
ATGTGGACGTTCGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG9160.14706071231328427No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG9000.14449196624667668No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT8470.13598299490103904No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT8430.13534080838438717No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC8250.1324509690594536No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA8220.1319693291719647No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT8070.12956112973452008No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC8040.12907948984703116No Hit
GTCTGGGAGAGACTTCACCCTCACCATCAGCAGACTGGAGCCTGAAGATT8000.12843730333037928No Hit
ATGTTGGCTCAATGTGGACGTTCGGCCAGGGGACCAAGGTGGAGATCAAA7880.1265107437804236No Hit
CTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGACCTGGT7860.12618965052209763No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG7640.12265762468051222No Hit
CCCTGTCTCTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGACTGTTAGCAACAATTACTTGG7350.11800177243478596No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA7290.1170384926598081No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA7260.11655685277231918No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7220.11591466625566729No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7160.11495138648068945No Hit
GTACCAGAAGAGACTTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGTGTCTACCAGGTCCACTGGCATCCCAGA7000.11238264041408187No Hit
GTCTCTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA6980.11206154715575592No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC6950.111579907268267No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT6780.10885061457249644No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT6760.10852952131417049No Hit
GACTTCACCCTCACCATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTA6640.1066029617642148No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT6600.10596077524756291No Hit
GGATAGCAGGCATCTGGGGTTAAGGCTGTTTCCACCATTTTGGTCTCACCACCATATACGAGTGGGACCACAG6460.10371312243928127No Hit
GAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTT6450.1035525758101183No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC6390.10258929603514046No Hit
GAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCC6380.10242874940597747No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTT6360.10210765614765153No Hit
GGGTGAAGTCTCTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA6310.10130492300183666No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTC6310.10130492300183666No Hit
CACCATAGATGAGGAGCCTGGGAGCCTGGCCAAGTCTCTTCTGGTACCAGGCCAAGTAATTGTTGCTAACAGT6260.10050218985602179No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1350.051.21693441
TCGTATG1450.049.99524740
ACGAGAC1650.047.2461722
TGTCGTA306.400453E-445.8660638
AACGGGG701.5829573E-743.48768669
CCCACGA1800.043.30203619
AGACCGA1750.042.65069225
GCCGTCT1750.041.7834846
CACGAGA1850.040.3031321
ATGCCGT1950.039.18340344
TATACAC1950.038.2301643
GGTATCA4350.038.1702461
CTTATAC1900.037.452761
ACCGAGG2000.037.36441427
ATCTCCG2000.037.2714210
GTATCAA11350.037.020641
GACCGAG2050.036.42376726
CGAGGCT2100.035.6367829
TTATACA2000.035.580122
ACACATC2150.034.673876
Produced by FastQC (version 0.11.5)