FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686840

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686840
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences622872
Sequences flagged as poor quality0
Sequence length20-76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTACTGTCAGCAGTATGTTGGCTCAATGTGGACGTTCGGCCAGGGGA31040.49833673692187164No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT26200.42063216840699214No Hit
CAGTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGGGTGAAGTCTCTCCCAGACCCACTGCCA20550.3299233229299118No Hit
GTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCA18050.2897866656391683No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC17520.2812776942935306No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17410.2795116813727379No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC17410.2795116813727379No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC15530.24932891509009875No Hit
CATCTATGGTGTGTCTACCAGGTCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGAGAGACTTCAC15470.2483656353151209No Hit
GTGTATTACTGTCAGCAGTATGTTGGCTCAATGTGGACGTTCGGCCAGGGGACCAAGGTGGAGATCAAACGAA15140.24306759655274277No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTTTGGCCT13770.22107270835741533No Hit
CCATAGATGAGGAGCCTGGGAGCCTGGCCAAGTCTCTTCTGGTACCAGGCCAAGTAATTGTTGCTAACAGTCTGA13460.21609576285336313No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG13350.21432974993257042No Hit
CTCCTGCAGGGCCAGTCAGACTGTTAGCAACAATTACTTGGCCTGGTACC12120.19458251454552458No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC12100.19426142128719867No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT11400.18302315724579046No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA11080.1778856651125753No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG11030.17708293196676042No Hit
GTAATACACTGCAAAATCTTCAGGCTCCAGTCTGCTGATGGTGAGGGTGAAGTCTCTCCCAGACCCACTGCCA11010.17676183870843448No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT10850.1741930926418269No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT10850.1741930926418269No Hit
GTCTCCAGGCACCCTGTCTCTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGACTGTTAGCAA10710.17194543983354527No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGC10470.1680923207336339No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTT10400.16696849432949304No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTG10290.16520248140870036No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAG10210.16391810837539655No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC9970.16006498927548518No Hit
GTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCCCCTGGCCGAACGTC9640.15476695051310702No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCA9610.1542853106256181No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAG9600.15412476399645514No Hit
GCTCAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCT8950.14368923310086182No Hit
CTCCAGGCACCCTGTCTCTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGC8920.14320759321337287No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCT8890.14272595332588395No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT8890.14272595332588395No Hit
TCTATGGTGTGTCTACCAGGTCCACTGGCATCCCAGACAGGTTCAGTGGCAGTGGGTCTGGGAGAGACTTCACC8740.14031775388843934No Hit
CAGTTAGGACCCAGAGGGAACCATGGAAACCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATAC8510.13662518141769095No Hit
ATGTGGACGTTCGGCCAGGGGACCAAGGTGGAGATCAAACGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCG8380.13453807523857228No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATCTCCACCTTGGTCC8320.13357479546359444No Hit
GTCTGGGAGAGACTTCACCCTCACCATCAGCAGACTGGAGCCTGAAGATT8220.1319693291719647No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTA7870.1263501971512606No Hit
ATGTTGGCTCAATGTGGACGTTCGGCCAGGGGACCAAGGTGGAGATCAAA7860.12618965052209763No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG7830.1257080106346087No Hit
GTCTCTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGA7600.1220154381638603No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT7490.12024942524306759No Hit
CCCTGTCTCTGTCTCCAGGGGAAAGAGCCACCCTCTCCTGCAGGGCCAGTCAGACTGTTAGCAACAATTACTTGG7470.11992833198474165No Hit
CTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCAGTGGACCTGGT7370.1183228656931119No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTG7360.11816231906394893No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGCGAAGTCA7230.11607521288483026No Hit
CTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTC7230.11607521288483026No Hit
GGGTGAAGTCTCTCCCAGACCCACTGCCACTGAACCTGTCTGGGATGCCA7220.11591466625566729No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7050.11318537355989673No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA7040.11302482693073376No Hit
CCCCAGCGCAGCTTCTCTTCCTCCTGCTACTCTGGCTCCCAGATACCACCGGAGAAATTGTGTTGACGCAGTCTC6870.1102955342349632No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC6800.10917170783082239No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA6600.10596077524756291No Hit
GTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCAT6560.105318588730911No Hit
GTACCAGAAGAGACTTGGCCAGGCTCCCAGGCTCCTCATCTATGGTGTGTCTACCAGGTCCACTGGCATCCCAGA6380.10242874940597747No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCTAA252.361085E-456.1386850
TCGCTCA504.2769523E-544.2778869
CCACGTG2300.041.82578370
CTATTCC350.001217922340.24656752
GCGCCTA350.001251332340.02570749
AATGGTA5000.039.85399670
GGTATCA5750.038.8183171
GTATAAA350.001486049438.6502761
CCTTATA451.1257092E-437.5766531
GTATCAA13000.034.8595731
CGCCCTT400.002699007734.2256835
CGATCAG450.003267812532.91549766
GCCGTGA450.003559104232.3467663
GCATGCA850.003562366632.335970
ACGGGTC851.4275975E-731.85521116
CCTAAGC450.0041792431.30288352
TAAGTCA1103.331723E-431.2335470
GCCACGT1850.031.1141969
GAGTTTA1251.5825208E-1029.760711
TATCAAC15050.029.210072
Produced by FastQC (version 0.11.5)