FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686841

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686841
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1070501
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACGGCCGTGTCCGCAGTGGTCACAGATGTCAGTGTCAGGGACAGCTCCTTCCTGGCCCAGTCGAAAGA33960.31723464060285794No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG29290.2736102068097087No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG28080.2623070879896422No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT27480.2567022356821712No Hit
CCCCTGTCCTGGTCATCCATCAAGATAACAGGCGGCCCTCAGGGATCCCT27380.25576809363092606No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC26330.24595960209285186No Hit
CCTCTGAACTGACTCAGCCGCCCTCAGTGTCCGTGTCCCCAGGACAGACA24140.22550189117058272No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG23770.22204556558097563No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCGTGGTCCCCC23600.22045752409385883No Hit
GACTTAGGACGGTCAGCGTGGTCCCCCCGCCGAAAATTGCAGTGTCGCTG23070.2155065712222595No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA23030.2151329144017614No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCGTGGTCCCCCCGCCGAAAATTGC22330.20859392004304528No Hit
GTCCTGGTCATCCATCAAGATAACAGGCGGCCCTCAGGGATCCCTGACCG21850.20411003819706847No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC21290.19887884271009557No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT20110.18785596650540262No Hit
GGACAGGGGACTGGCCAGCCTTCTGTTGATACCAGGAAGCATATTTATCC19350.18075648691593935No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG19260.17991575906981871No Hit
GTCTATTACTGTGCGAAAATCACCGATACAAGTGGTTATTACAATGATGC18830.1758989482494645No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT18660.17431090676234773No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT18600.17375042153160064No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG17040.15917780553217606No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT17000.158804148711678No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC16780.15674903619893862No Hit
GACTATTACTGTCAGTCGTGGGACAGCGACACTGCAATTTTCGGCGGGGGGACCACGCTGACCGTCCTAAGTCAG16710.15609513676306702No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT16360.15282563958370896No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC16330.15254539696833538No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC16190.15123759809659215No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15950.14899565717360375No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG15880.14834175773773214No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15650.14619323101986828No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG15270.14264349122513664No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA15160.14161593496876695No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC15110.14114886394314438No Hit
CCCCAGGACAGACAGCCAGCATCACCTGCTCTGGAGATATTTTGGGGGAT15070.1407752071226463No Hit
CAGCCACTCTGACCATCCGCGAGACACAAGTTGTGGATGAGTCTGACTATTACTGTCAGTCGTGGGACAGCGACA14490.13535718322542437No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT14310.13367572753318305No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14230.13292841389218693No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13950.13031281614870047No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG13850.12937867409745532No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC13530.12638941953347077No Hit
GTAATAGTCAGACTCATCCACAACTTGTGTCTCGCGGATGGTCAGAGTGG13490.1260157627129727No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC13310.12433430702073142No Hit
CTATTACTGTCAGTCGTGGGACAGCGACACTGCAATTTTCGGCGGGGGGACCACGCTGACCGTCCTAAGTCAGCC12830.11985042517475462No Hit
GACCAGGACAGGGGACTGGCCAGCCTTCTGTTGATACCAGGAAGCATATT12780.11938335414913204No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA12710.11872945471326042No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT12690.1185426263030114No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12630.11798214107226429No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT12490.11667434220052106No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGCGTC12430.11611385696977397No Hit
CAGTAATAGTCAGACTCATCCACAACTTGTGTCTCGCGGATGGTCAGAGT12000.11209704614941976No Hit
ACAGAAGGCTGGCCAGTCCCCTGTCCTGGTCATCCATCAAGATAACAGGCGGCCCTCAGGGATCCCTGACCGATT11840.1106024188674275No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC11740.10966827681618233No Hit
GTCGTGGGACAGCGACACTGCAATTTTCGGCGGGGGGACCACGCTGACCGTCCTAAGTCAGCCCAAGGCTGCCCC11730.10957486261105782No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG11700.10929461999568425No Hit
GAGTGGCTGTGTTCCCAGTCTTGTAGCCAGAGAATCGGTCAGGGATCCCT11600.1083604779444391No Hit
CTCCTGGACCCGCCAGAATGGCGAAGCTGTGAAAACCCACACCAACATCTCCGAGAGCCACCCCAATGCCACTTT11520.10761316430344296No Hit
GGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGC11450.10695926486757135No Hit
GTCCCACGACTGACAGTAATAGTCAGACTCATCCACAACTTGTGTCTCGC11410.10658560804707329No Hit
ACGTTGTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGT11340.10593170861120167No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT11330.10583829440607714No Hit
GCTATCTGCCTTCCAGGCCACTGTCACGGCTCCCGGGTAGAAGTCACTTA10840.10126099835497585No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC10790.10079392732935326No Hit
CTACAAGACTGGGAACACAGCCACTCTGACCATCCGCGAGACACAAGTTG10750.1004202705088552No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)