FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004686842

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686842
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1070501
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAATAGACGGCCGTGTCCGCAGTGGTCACAGATGTCAGTGTCAGGGACAGCTCCTTCCTGGCCCAGTCGAAAGA30400.2839791835785301No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG28130.2627741590152648No Hit
CCCCTGTCCTGGTCATCCATCAAGATAACAGGCGGCCCTCAGGGATCCCT26560.24810812881071576No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT26070.24353083275961443No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG25510.2382996372726415No Hit
CCTCTGAACTGACTCAGCCGCCCTCAGTGTCCGTGTCCCCAGGACAGACA25430.23755232363164538No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24600.2297989446063105No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC24480.2286779741448163No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG24300.226996518452575No Hit
GTGCAGGGCCACCCCCTTGGGCCGGGAGATGGTCTGCTTCAGTGGCGAGGGCAGGTCTGTGTGGGTCACGGTGCA24140.22550189117058272No Hit
GACTTAGGACGGTCAGCGTGGTCCCCCCGCCGAAAATTGCAGTGTCGCTG23410.21868265419649308No Hit
GCCTTGGGCTGACTTAGGACGGTCAGCGTGGTCCCCCCGCCGAAAATTGC21920.20476393763294007No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC21460.20046688419721234No Hit
GTCCTGGTCATCCATCAAGATAACAGGCGGCCCTCAGGGATCCCTGACCG21240.19841177168447294No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGACTTAGGACGGTCAGCGTGGTCCCCC20310.18972425060789294No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG19870.18561402558241422No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT19110.17851454599295097No Hit
GGACAGGGGACTGGCCAGCCTTCTGTTGATACCAGGAAGCATATTTATCC18660.17431090676234773No Hit
GCCCTGCACAGGCCCGATGTCTACTTGCTGCCACCAGCCCGGGAGCAGCT18550.17328335050597804No Hit
TCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG18110.16917312548049931No Hit
GGTGTAGGTCTCCCCCGTGTTCCATTCCTCTTCGGACACGGTCAGGATGCTGTGGGCGAAGTACCGGCCTGGGGC17540.1638485157884019No Hit
GTCTATTACTGTGCGAAAATCACCGATACAAGTGGTTATTACAATGATGC17320.16179340327566252No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG16760.1565622077886896No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT16730.15628196517331605No Hit
GACTATTACTGTCAGTCGTGGGACAGCGACACTGCAATTTTCGGCGGGGGGACCACGCTGACCGTCCTAAGTCAG16000.14946272819922635No Hit
GTCCTAAGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTC15860.14815492932748311No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15720.14684713045573988No Hit
GTATGTGACCAGCGCCCCAATGCCTGAGCCCCAGGCCCCAGGCCGGTACTTCGCCCACAGCATCCTGACCGTGTC15300.1429237338405102No Hit
ACCTATGACAGCGTGACCATCTCCTGGACCCGCCAGAATGGCGAAGCTGT14830.13853326619965792No Hit
CCCCAGGACAGACAGCCAGCATCACCTGCTCTGGAGATATTTTGGGGGAT14670.13703863891766566No Hit
CTCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGTGACTTCTACC14590.13629132527666954No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT14460.13507694061005082No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC14050.13124695819994564No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG13980.130593058764074No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA13950.13031281614870047No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGG13870.12956550250770435No Hit
GTCCTGGACCTCCTGTGCAAGAACATGAAACATCTGTGGTTCTTCCTTCTCCTGGTGGCAGCTCCCAGATGCGTC13840.12928525989233078No Hit
CAGCCACTCTGACCATCCGCGAGACACAAGTTGTGGATGAGTCTGACTATTACTGTCAGTCGTGGGACAGCGACA13710.12807087522571206No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG13700.12797746102058757No Hit
GTCGTGGGACAGCGACACTGCAATTTTCGGCGGGGGGACCACGCTGACCGTCCTAAGTCAGCCCAAGGCTGCCCC13360.124801378046354No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGA13310.12433430702073142No Hit
GTAATAGTCAGACTCATCCACAACTTGTGTCTCGCGGATGGTCAGAGTGG13260.12386723599510883No Hit
CTCCATGTGTGTCCCCGATCAAGACACAGCCATCCGGGTCTTCGCCATCCCCCCATCCTTTGCCAGCATCTTCCT12690.1185426263030114No Hit
ACACTGGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGC12670.11835579789276236No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT12560.11732824163639267No Hit
GAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGAT12530.11704799902101913No Hit
CTATTACTGTCAGTCGTGGGACAGCGACACTGCAATTTTCGGCGGGGGGACCACGCTGACCGTCCTAAGTCAGCC12420.11602044276464946No Hit
GACCAGGACAGGGGACTGGCCAGCCTTCTGTTGATACCAGGAAGCATATT12380.11564678594415138No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC12060.11265753138016686No Hit
GTACAGGGTGGGTTTACCGGTGGACTTGTCCACGGTCCTCTCGGTGACCCTGTTGGGCAGGGCCTCATGGGCCAC12000.11209704614941976No Hit
GAGTGGCTGTGTTCCCAGTCTTGTAGCCAGAGAATCGGTCAGGGATCCCT11940.11153656091867266No Hit
GCTCTAGTCTCCCGTGGTGGGGGGTGAGGGTTGAGAACCTATGAACATTC11620.10854730635468812No Hit
ATCTGAGCCTGACGCCTGAGCAGTGGAAGTCCCACAGAAGCTACAGCTGC11480.10723950748294489No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA11420.1066790222521978No Hit
CTTATGAGACACACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGA11050.1032226966625907No Hit
ATCTCACCCCGTTGACACGGTTAGTTTGCATGCACACACAGAGCGGCCAG10910.10191489779084746No Hit
ACAGAAGGCTGGCCAGTCCCCTGTCCTGGTCATCCATCAAGATAACAGGCGGCCCTCAGGGATCCCTGACCGATT10880.10163465517547392No Hit
CAGTAATAGTCAGACTCATCCACAACTTGTGTCTCGCGGATGGTCAGAGT10840.10126099835497585No Hit
GTCCCTCACCTGCGCTGTCTCTGGTGACAACATCCACAATCATCACTACG10830.10116758414985133No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)