FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686843

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686843
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1247926
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATACTGGGTCTTCCTACTACAACCCGTCTCTCAAGAGTCGACTCACCTTTTTCGTCGACACGTCCAGGAA33910.2717308558360031No Hit
CCTATGAACTGACTCAGCCACCCTCACTGTCCGTGTCCCCAGGGCAGACG33170.26580101704748516No Hit
GCCCAAGGCTGCCCCCTCGGTCACCCTGTTCCCGCCCTCCTCTGAGGAGC32500.26043210895517843No Hit
GTCTCTGGTGTCTCCACCAGTAGTCCTGATTACTACTGGGACTGGGTCCG31120.24937376094415856No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAATCAC30410.24368432102544543No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACCCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG29290.23470942988606697No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG27910.22365108187504706No Hit
ATAATATATAGCCGTGTCTGCGGCGCTCAGAGAACTCAGCCTCAGGGAGA27550.2207662954373897No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC26390.21147087247160487No Hit
GTATAATAGATGGACCCAACCCACTCCAGTCCCTTCCCGGGGGGCTGGCGGACCCAGTCCCAGTAGTAATCAGGA25940.20786488942453316No Hit
CACCTGCACTGTCTCTGGTGTCTCCACCAGTAGTCCTGATTACTACTGGG25750.2063423632491029No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT25580.2049801029868758No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAATCACGGTGTAGGTG24190.19384162201925437No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG24060.19279989358343363No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT23770.19047603784198744No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA23450.18791178323073643No Hit
GTGTAGGTGTCCCACGCCTGACAGTAATAGTCAGACTCATCTATAGGTTGGGTCCCTCTGATGGTCAGAGTGG21930.17573157382729426No Hit
GTGTGGTGGTCTCCACTCCCGCCGTGACGGGGCTGCTATCTGCCTTCCAG21770.17444944652166874No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC21680.1737282499122544No Hit
TCCTACTACAACCCGTCTCTCAAGAGTCGACTCACCTTTTTCGTCGACACGTCCAGGAACCACTTCTCCCTGAGG21180.16972160208217474No Hit
CACCTACACCGTGATTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCAC21090.1690004054727604No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACCCTGTTCCCGCCCTC21040.16859974068975245No Hit
CAATAATATATAGCCGTGTCTGCGGCGCTCAGAGAACTCAGCCTCAGGGA21020.16843947477654925No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC20760.1663560179049078No Hit
ATATATTATTGTGCGAGACGGGGGGCCGACGTGCAAGGGCGGAATGAGAT19650.15746125972213096No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG19650.15746125972213096No Hit
CCTCAGAGGAGGGCGGGAACAGGGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC19610.15714072789572459No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG19090.15297381415244174No Hit
CATCTATAGGTTGGGTCCCTCTGATGGTCAGAGTGGCTGTGTTCCCAGAG18510.14832610266954932No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC17950.1438386570998601No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT17760.14231613092442982No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG17590.14095387066220272No Hit
GTCCAGGATCTCATGCGCAAAAAAATGAAGCACCTGTGGTTCTTCCTCCTGCTGGTGGCGGCTCCCAGATGGGT17270.13838961605095174No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA17170.13758828648493582No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG16380.13125778291340995No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG16240.13013592152098763No Hit
GTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCCGG16180.12965512378137806No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC16160.12949485786817488No Hit
GCTCAGTAGCAGGTGCCGTCCACCTCCGCCATGACAACAGACACATTGAC16040.12853326238895577No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC16010.12829286351915098No Hit
CTATAGATGAGTCTGACTATTACTGTCAGGCGTGGGACACCTACACCGTGATTTTCGGCGGAGGGACCAAGCTGA15930.12765179986633823No Hit
GGACTGGAGTGGGTTGGGTCCATCTATTATACTGGGTCTTCCTACTACAACCCGTCTCTCAAGAGTCGACTCAC15710.12588887482110317No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT15480.12404581681926653No Hit
ATATTATTGTGCGAGACGGGGGGCCGACGTGCAAGGGCGGAATGAGATCCCAGACTGGGGCCAGGGAATTCAGGT15430.12364515203625856No Hit
CTACTGGGACTGGGTCCGCCAGCCCCCCGGGAAGGGACTGGAGTGGGTTGGGTCCATCTATTATACTGGGTCTTC15380.1232444872532506No Hit
CCCTCACTGTCCGTGTCCCCAGGGCAGACGGCCACCATCACCTGCTCTGGAGATAATTTGGGGGATAAATTTGC15360.12308422134004741No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAATCACGGTGTAGGTGTCCCACGCCTGACAGTAATAGTCAGA15280.12244315768723465No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG15120.12116103038160918No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA14830.11883717464016297No Hit
GACTATTACTGTCAGGCGTGGGACACCTACACCGTGATTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG14660.11747491437793588No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT14640.11731464846473268No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT14540.11651331889871676No Hit
CCTTGGGGCTGGTCGGGGATGCTGCGGAGACCATGACCTGAATTCCCTGGCCCCAGTCTGGGATCTCATTCCGCC14520.11635305298551356No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT14380.11523119159309125No Hit
GTAGTAGGAAGACCCAGTATAATAGATGGACCCAACCCACTCCAGTCCCTTCCCGGGGGGCTGGCGGACCCAGTC14350.11499079272328648No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCGTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC14320.1147503938534817No Hit
GTCCGTGTCCCCAGGGCAGACGGCCACCATCACCTGCTCTGGAGATAATT13720.10994241645738609No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGCGGGAACAGGGTGACCGAGGGGGCAGCCTTGGG13610.10906095393476856No Hit
CCCCAGGGCAGACGGCCACCATCACCTGCTCTGGAGATAATTTGGGGGAT13560.1086602891517606No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG13470.10793909254234627No Hit
CAGTATGCTGGTCACAGCGAAGGTGGTGGTGCCCTGGCTGGGCTCCTGCC13470.10793909254234627No Hit
CAGCCACTCTGACCATCAGAGGGACCCAACCTATAGATGAGTCTGACTATTACTGTCAGGCGTGGGACACCTACA13320.10673709819332235No Hit
ACCTATAGATGAGTCTGACTATTACTGTCAGGCGTGGGACACCTACACCGTGATTTTCGGCGGAGGGACCAAG13230.10601590158390803No Hit
ATATATAGCCGTGTCTGCGGCGCTCAGAGAACTCAGCCTCAGGGAGAAGT13220.10593576862730643No Hit
GTCTTGGACTCGGGGTAGGCAGCAGTGCAAGTGAAGGTCTTCCCATGGTT13140.10529470497449367No Hit
GTCACGGACTTGCCGGCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCT13120.1051344390612905No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGCGGAGACCATGACCTGAATTCCCTGG13110.10505430610468891No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG13080.10481390723488411No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCGTGA12780.10240991853683631No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12770.10232978558023473No Hit
GCTATATATTATTGTGCGAGACGGGGGGCCGACGTGCAAGGGCGGAATGAGATCCCAGACTGGGGCCAGGGAATT12700.10176885488402357No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12680.1016085889708204No Hit
GTAATAGTCAGACTCATCTATAGGTTGGGTCCCTCTGATGGTCAGAGTGG12570.10072712644820286No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGCT1100.046.76517528
TCGTATG551.4552825E-743.96499640
TACGCTG1250.041.17317629
ACTACGC1250.041.13025727
CGAGACT1250.041.1038823
GTATCAA22100.040.6030271
CGTATGC459.861154E-538.4349541
GACTACG1400.036.7146126
TATCAAC24600.036.615932
ATCAACG25700.035.4485053
CAACGCA26050.035.1037035
AACGCAG26450.034.8318066
TCAACGC26200.034.7720074
AGACTAC1500.034.26284825
TATACAC1650.033.211273
TCTCGTA958.8475645E-932.66510438
ACGCAGA28350.032.6182067
AGAGTAC28350.032.3765911
CGCAGAG28700.032.101098
CAGAGTA28950.031.70557410
Produced by FastQC (version 0.11.5)