FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004686844

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004686844
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1247926
Sequences flagged as poor quality0
Sequence length20-76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTATACTGGGTCTTCCTACTACAACCCGTCTCTCAAGAGTCGACTCACCTTTTTCGTCGACACGTCCAGGAA33180.2658811500040868No Hit
GCCCAAGGCTGCCCCCTCGGTCACCCTGTTCCCGCCCTCCTCTGAGGAGC32460.26011157712877203No Hit
GTCTCTGGTGTCTCCACCAGTAGTCCTGATTACTACTGGGACTGGGTCCG30910.24769096885552508No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAATCAC29310.23486969579927017No Hit
CCTATGAACTGACTCAGCCACCCTCACTGTCCGTGTCCCCAGGGCAGACG26570.2129132656904336No Hit
GTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAACTTCCCACCCAGCCAGGATGCCTCCGGGGAC26450.21195167021121447No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACCCTGTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGG26340.21107020768859694No Hit
ATAATATATAGCCGTGTCTGCGGCGCTCAGAGAACTCAGCCTCAGGGAGA25550.20473970411707104No Hit
GTATAATAGATGGACCCAACCCACTCCAGTCCCTTCCCGGGGGGCTGGCGGACCCAGTCCCAGTAGTAATCAGGA24770.1984893335021468No Hit
CACCTGCACTGTCTCTGGTGTCTCCACCAGTAGTCCTGATTACTACTGGG24590.19704694028331807No Hit
GTGTGGTGGTCTCCACTCCCGCCGTGACGGGGCTGCTATCTGCCTTCCAG24530.19656614254370852No Hit
GTGTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGT22850.18310380583464084No Hit
GTGCAGTGACAGTCGGGGGTGGCAGCATGAGGGAGATGGGGTAGGTGGAG22560.1807799500931946No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT22400.17949782278756915No Hit
GTCACTGCACCGACCGGCCCTCGAGGACCTGCTCTTAGGTTCAGAAGCGAACCTCACGTGCACACTGACCGGC22300.1786964932215532No Hit
GTGTAGGTGTCCCACGCCTGACAGTAATAGTCAGACTCATCTATAGGTTGGGTCCCTCTGATGGTCAGAGTGG22220.17805542956874046No Hit
AGGTCACACTGAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCG21840.1750103772178799No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAATCACGGTGTAGGTG21750.17428918060846557No Hit
TCCTACTACAACCCGTCTCTCAAGAGTCGACTCACCTTTTTCGTCGACACGTCCAGGAACCACTTCTCCCTGAGG21220.1700421339085811No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCTCTAGTCTCC20500.1642725610332664No Hit
GTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACCCTGTTCCCGCCCTC20400.16347123146725046No Hit
CACCTACACCGTGATTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCAC20360.16315069964084408No Hit
CAATAATATATAGCCGTGTCTGCGGCGCTCAGAGAACTCAGCCTCAGGGA19580.1569003290259198No Hit
GTCACATCCTGGCTGGGATTCGTGTAGTGCTTCACGTGGCATGTCACGGA19480.15609899945990388No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGGAAGAGCG19290.15457647328447358No Hit
GTTCAAGGACCACCTGAGCGTGACCTCTGTGGCTGCTACAGCGTGTCCAG19080.15289368119584013No Hit
ATATATTATTGTGCGAGACGGGGGGCCGACGTGCAAGGGCGGAATGAGAT18280.14648304466771267No Hit
GTCCAGGGCTTCTTCCCCCAGGAGCCACTCAGTGTGACCTGGAGCGAAAGCGGACAGGGCGTGACCGCCAGAAA18050.14463998666587602No Hit
CATCTATAGGTTGGGTCCCTCTGATGGTCAGAGTGGCTGTGTTCCCAGAG17980.14407905596966486No Hit
GCCCCAAGGTCTTCCCGCTGAGCCTCTGCAGCACCCAGCCAGATGGGAAC17750.14223599796782824No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAG17740.14215586501122662No Hit
GTCCAGGATCTCATGCGCAAAAAAATGAAGCACCTGTGGTTCTTCCTCCTGCTGGTGGCGGCTCCCAGATGGGT17660.14151480135841388No Hit
CCTCAGAGGAGGGCGGGAACAGGGTGACCGAGGGGGCAGCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTC16880.1352644307434896No Hit
CTGCAGCACCCAGCCAGATGGGAACGTGGTCATCGCCTGCCTGGTCCAGG16600.13302070795864498No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGG16500.13221937839262907No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16210.12989552265118284No Hit
GGACTGGAGTGGGTTGGGTCCATCTATTATACTGGGTCTTCCTACTACAACCCGTCTCTCAAGAGTCGACTCAC15930.12765179986633823No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTCT15830.1268504703003223No Hit
CCTGTACACCACGAGCAGCCAGCTGACCCTGCCGGCCACACAGTGCCTAG15620.12516767821168884No Hit
GCCCTGGACCAGGCAGGCGATGACCACGTTCCCATCTGGCTGGGTGCTGC15610.12508754525508725No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG15380.1232444872532506No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTT15070.1207603655986012No Hit
ATATTATTGTGCGAGACGGGGGGCCGACGTGCAAGGGCGGAATGAGATCCCAGACTGGGGCCAGGGAATTCAGGT15040.12051996672879642No Hit
GTGCCTAGCCGGCAAGTCCGTGACATGCCACGTGAAGCACTACACGAATCCCAGCCAGGATGTGACTGTGCCCTG15040.12051996672879642No Hit
GCTAGGCACTGTGTGGCCGGCAGGGTCAGCTGGCTGCTCGTGGTGTACAG14850.11899744055336614No Hit
CTATAGATGAGTCTGACTATTACTGTCAGGCGTGGGACACCTACACCGTGATTTTCGGCGGAGGGACCAAGCTGA14590.11691398368172473No Hit
GTGTGCACGTGAGGTTCGCTTCTGAACCTAAGAGCAGGTCCTCGAGGGCC14590.11691398368172473No Hit
GAGTGGCTCCTGGGGGAAGAAGCCCTGGACCAGGCAGGCGATGACCACGT14530.11643318594211516No Hit
CCCTCACTGTCCGTGTCCCCAGGGCAGACGGCCACCATCACCTGCTCTGGAGATAATTTGGGGGATAAATTTGC14500.11619278707231037No Hit
GACTATTACTGTCAGGCGTGGGACACCTACACCGTGATTTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAG14370.11515105863648967No Hit
GCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCA14340.11491065976668488No Hit
GTCCGTGTCCCCAGGGCAGACGGCCACCATCACCTGCTCTGGAGATAATT14070.11274706993844186No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCGTGACGGGGCTGCTATCTGCCTTCCAGGCCACTGTCACGGCTC14060.11266693698184027No Hit
GTAGTAGGAAGACCCAGTATAATAGATGGACCCAACCCACTCCAGTCCCTTCCCGGGGGGCTGGCGGACCCAGTC13910.11146494263281637No Hit
GGCTCAGATAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCGTGA13340.10689736410652556No Hit
GTACAGGTCCCCGGAGGCATCCTGGCTGGGTGGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGT13280.10641656636691599No Hit
GTGCTGCAGAGGCTCAGCGGGAAGACCTTGGGGCTGGTCGGGGATGCTGCGGAGACCATGACCTGAATTCCCTGG13280.10641656636691599No Hit
CCTTGGGGCTGGTCGGGGATGCTGCGGAGACCATGACCTGAATTCCCTGGCCCCAGTCTGGGATCTCATTCCGCC13170.10553510384429847No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTCTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTG13150.10537483793109528No Hit
CAGCCACTCTGACCATCAGAGGGACCCAACCTATAGATGAGTCTGACTATTACTGTCAGGCGTGGGACACCTACA12960.10385231175566499No Hit
ATATATAGCCGTGTCTGCGGCGCTCAGAGAACTCAGCCTCAGGGAGAAGT12940.10369204584246182No Hit
CTACTGGGACTGGGTCCGCCAGCCCCCCGGGAAGGGACTGGAGTGGGTTGGGTCCATCTATTATACTGGGTCTTC12880.10321124810285226No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAG12850.10297084923304746No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAT12840.10289071627644587No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT12820.10273045036324269No Hit
ACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAAAATCACGGTGTAGGTGTCCCACGCCTGACAGTAATAGTCAGA12780.10240991853683631No Hit
GGATGTGACTGTGCCCTGCCCAGTTCCCTCAACTCCACCTACCCCATCTCCCTCAACTCCACCTACCCCATCTCC12740.10208938671042996No Hit
GTCCAAGACCCCGCTAACCGCCACCCTCTCAAAATCCGGAAACACATTCC12660.1014483230576172No Hit
GAGTGGCTGTGTTCCCAGAGTTGGAGCCAGAGAATCGCTCAGGGATCCCT12660.1014483230576172No Hit
CCCCAGGGCAGACGGCCACCATCACCTGCTCTGGAGATAATTTGGGGGAT12620.10112779123121084No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAA200.00675935852.87464558
ATTAGCG306.5942184E-445.5929683
TAGTACG306.5942184E-445.5929684
ATACGTA1001.0913936E-1137.64587411
GAATACG1001.0913936E-1137.6383329
GTATCAA19250.036.5986631
AATACGT1103.092282E-1134.2194110
TATAGGG501.9613035E-434.194732
ATTGTAC400.00271207234.1947253
TATCAAC22000.032.01872
TACGTAC1208.54925E-1131.37156112
GTGGTAT2450.030.710391
GTAATCG450.00480934430.41969116
TCAACGC23700.030.1548444
ATCAACG23750.029.947383
CAACGCA23700.029.8662825
AACGCAG24100.029.6579136
GACGAAA13300.027.8900770
CGCAGAG25550.027.8532128
ACGCAGA25700.027.8193117
Produced by FastQC (version 0.11.5)